Chitosan double-network quick response-type injectable hydrogel and preparation method thereof

Abstract

The invention relates to chitosan double-network quick response-type injectable hydrogel and a preparation method thereof. The injectable hydrogel is prepared from chitosan or derivatives of the chitosan and sodium glycerophosphate serving as raw materials, and dialdehyde compounds or diepoxy compounds serving as crosslinking agents, wherein the mass ratio of the chitosan to the sodium glycerophosphate is 1:(0.5-10); the mass ratio of the crosslinking agents to the chitosan is (0.01-1):1. The method comprises the following steps: evenly mixing a hydrochloric acid aqueous solution of the chitosan with a sodium glycerophosphate solution in the environment at -1 to 5 DEG C, so as to obtain a solution of which the pH is 7-7.2, and evenly mixing the solution with a crosslinking solution at 35-37 DEG C. Transformation from a sol state to a gel state can be quickly finished under a specific condition, the process is irreversible, and the transition time is controllable. The chitosan double-network quick response-type injectable hydrogel has the advantages of simple reaction condition, short response time, good thermal stability of gel, high mechanical strength, good biocompatibility and the like. The chitosan double-network quick response-type injectable hydrogel is applied to the fields such as tissue filling and repairing, biological scaffolds, scar resistance and anti-adhesion, biogel, and quickly molding or three-dimensional printing materials and the like.

Description

technical field [0001] The invention relates to a chitosan double-network fast-response injectable hydrogel and a preparation method thereof. Combining temperature-sensitive gels with chemical cross-linking methods to prepare chitosan double-network fast-response injectable hydrogels. By this method, the obtained chitosan double-network rapid-response gel can quickly complete the transition from the sol state to the gel state under specific conditions, the process is irreversible, and the transition time is controllable. The invention has the advantages of simple reaction conditions, short response time, good thermal stability of the gel, high mechanical strength, good biocompatibility and the like. The invention relates to the fields of tissue filling and repair, biological scaffolds, anti-scar and anti-adhesion, biological glue, rapid prototyping or three-dimensional printing materials and the like. Background technique [0002] Chitosan (chitosan, CS) is the product of...

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Problems solved by technology

But the shortcoming of chitosan thermosensitive gel is that response time is longer, and its solidification time under 37 ℃ physiological environment is not less than 8 minutes (Wu Guangsheng et al., Journal of Dental Endodontics and Periodontology, 2010, 20 (11) , 614); and the mechanical properties of thermosensitive gels are poor, because the formation of the gel network relies on physical interactions such as hydrogen bonds and electrostatic attraction between chitosans, and there is no cross-linking of chemical bonds

[0004] And the chitosan gel that adopts chemical cross-linking to make, although mechanical strength is higher, reaction time is too long, take classic cross-linking agent glutaraldehyde as example, its gelation time is not less than one hour (Li Zhanyong et al., Ion exchange and adsorption, 1999, 15(5), 425), the gelation time is too long, which is not conducive to rapid prototyping

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