Process for preparing magnolia officinalis total phenol extract by adsorptive separation

Abstract

The invention provides a process for extracting two isomeride components in magnolia officinalis, namely honokiol and magnolol. The process comprises the steps of preparing an adsorption liquid, adsorbing by macroporous resin, balancing after adsorption, desorbing magnolia officinalis total phenol and post-treating the desorption solution. The process can be used for overcoming the interference of co-existing alkaloid when the magnolia officinalis total phenol extract is prepared by adsorptive separation, a method for preparing the adsorbent which is extracted from an organic solvent by acid-alkali isolation is provided, a continuous process for pre-separating alkaloid and extracting high-purity magnolia officinalis total phenol adsorptive resin is established, and the magnolia officinalis total phenol extract with purity higher than 70 percent is obtained by an 'adsorption-elution' process flow. The adsorptive resin method extraction process is convenient to operate, the resin can be recycled, and the process is applicable to large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of monomeric active ingredients in natural plants. Especially the extraction process of honokiol and magnolol, two isomer components in magnolia bark. Background technique [0002] Magnolia officinalis is a commonly used traditional Chinese medicine. It was first recorded in "Shen Nong's Materia Medica", and was listed as a middle grade. It was recorded in successive dynasties of herbal medicines and "Chinese Pharmacopoeia". The 2010 edition of "Chinese Pharmacopoeia" stipulates that magnolia officinalis is the dry dry bark and root of Magnolia officinalis Rehd. et Wils. or Magnolia officinalis Rehd. et Wils. Var. biloba Rehd. et Wils. Bark and branches. Modern pharmacological experiments have proved that magnolia bark has broad-spectrum antibacterial, anti-tumor, anti-inflammatory, cardiovascular and cerebrovascular protection, anti-ulcer and anti-coagulant effects. [0003...

AI Technical Summary

Problems solved by technology

Ultrasonic extraction method has fast extraction speed and short time, but its noise is large, and the purity of the extract is not high; the subsequent steps of hot-dipping extraction method are cumbersome, and the steps of extraction, recrystallization and other steps use a large amount of organic solvent, and the loss of extract is serious; high-speed countercurrent chromatography The purity of magnolol and honokiol can be met by separation and preparation of magnolol and honokiol, but the preparation amount is small, so it is not suitable for industrial expansion production; while the supercritical extraction method is complicated in equipment, and the initial equipment investment is large, which limits supercritical extraction. Large-scale industrial application of the method

The process of solvent extraction is cumbersome, there are many operation steps, and the loss of solvent is also large

And above-mentioned method all faces the problem that contains alkaloid component in the total magnolol of extraction gained, and the combination of residual alkaloid component and magnolol makes the simultaneous extraction of magnolol and honokiol very difficult. Difficult to achieve

[0006] In recent years, the macroporous adsorption resin method has played an increasingly important role in the separation and purification of natural plant active ingredients due to its advantages of simple process operation, environmental friendliness, reusable adsorption resin, and low separation cost. The extraction process has also become a mature method for widely used industrial production and preparation of natural products. In the extraction of active ingredients of magnolia bark, there are also reports on the separation of adsorption resins, but so far, in the reported methods, the total magnolols prepared The purity of the extract is relatively low, such as Zhao Chunying etc. use macroporous resin DM-301 to increase the content of magnolol in the traditional Chinese medicine Magnolia Bark from 7.56% to 15.26% [Zhao Chunying, Mao Xiaoxia, Zhang Ziqian. Macroporous adsorption resin separation and purification of magnolol Method research [J]. Journal of Chengde Medical College, 2009,26(4):358-360], Ye Hui et al. reduced the adsorption of some impurities in Magnolia officinalis on the resin by adjusting the ethanol concentration of the adsorption solution. outflow, thereby preparing the total phenolic extract of magnolia bark with a purity of 46.0%, but at the same time, the alkaloids present in magnolia bark also enter the adsorption solution due to the increase in ethanol concentration, and enter the final stage with the adsorption-desorption process. The product has caused the problem that the alkaloid components in the total phenols of magnolol are difficult to separate, which inevitably restricts the further pharmacological research and medicinal development of magnolol [Ye Hui, Ran Xiaohui, Yu Kai. Study on the process of Magnolia officinalis extract [J ].Pharmaceutical Herald.2008,27(8):974-975]

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