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Method for extracting vitexin and trigonelline from fenugreek

A technology of fenugreek and vitexin, which is applied in the direction of organic chemistry and the like, can solve the problems of complex and complicated components, and the medicinal effect of vitexin is difficult to achieve the expected effect, and achieves the effect of simplifying the extraction process and improving the extraction rate.

Inactive Publication Date: 2014-07-16
成都合盛生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the process of using vitexin and trigonelline extracted from herbal plants is relatively complicated, and the composition is complex, and the purity is difficult to reach 98% (HPLC) , making it difficult for the extracted vitexin to achieve the desired effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take a certain amount of fenugreek seeds and fenugreek leaves and wash them; place the fenugreek seeds at 25 MPa and 90° C. for 1 hour to make them fully puffed and then crush them to obtain crushed fenugreek seeds; crush the fenugreek leaves into powder, and passed through a 40-mesh sieve to obtain the pulverized fenugreek leaves; add 6 times the total weight of the purified water to the pulverized fenugreek seeds and fenugreek leaves obtained above, soak for 0.5h, and boil 10 minutes to obtain the filtrate; add pure water 7 times the weight of the filter residue to the above filter residue, soak for 0.5h, boil for 10 minutes to obtain the filtrate; mix the above two filtrates, stir evenly, concentrate into a paste, and obtain Aqueous extract containing trigonelline and vitexin.

[0019] Add hydrochloric acid to the aqueous extract containing trigonelline and vitexin obtained above, adjust the pH of the solution to PH=0.1, and hydrolyze for 1 hour. After the crystal A ...

Embodiment 2

[0022] Take a certain amount of fenugreek seeds and fenugreek leaves and wash them; place the fenugreek seeds at 25 MPa and 100°C for 1 hour to make them fully puffed and then crush them to obtain crushed fenugreek seeds; crush the fenugreek leaves into powder, and passed through a 40-mesh sieve to obtain the pulverized fenugreek leaves; add 7 times the total weight of the purified water to the pulverized fenugreek seeds and fenugreek leaves obtained above, soak for 0.6h, and boil 15 minutes to obtain the filtrate; add pure water 7 times the weight of the filter residue to the above filter residue, soak for 0.7h, boil for 10 minutes to obtain the filtrate; mix the above two filtrates, stir evenly, concentrate into a paste, and obtain Aqueous extract containing trigonelline and vitexin.

[0023] Add hydrochloric acid to the aqueous extract containing trigonelline and vitexin obtained above, adjust the pH of the solution to PH=0.3, and hydrolyze for 2 hours. After crystal A is f...

Embodiment 3

[0026] Take a certain amount of fenugreek seeds and fenugreek leaves and wash them; place the fenugreek seeds at 25MPa and 110°C for 2 hours, make them fully puffed, and then crush them to obtain crushed fenugreek seeds; put the fenugreek leaves Pulverize into powder and pass through a 40-mesh sieve to obtain the crushed fenugreek leaves; add 8 times the total weight of the purified water to the obtained fenugreek seeds and fenugreek leaves, soak for 1 hour, and boil 15 minutes to obtain the filtrate; add pure water 7 times the weight of the filter residue to the above filter residue, soak for 0.8h, boil for 20 minutes to obtain the filtrate; mix the above two filtrates, stir evenly, concentrate into a paste, and obtain Aqueous extract containing trigonelline and vitexin.

[0027] Add hydrochloric acid to the aqueous extract containing trigonelline and vitexin obtained above, adjust the pH of the solution to PH=0.35, and hydrolyze for 2 hours. After crystal A is fully analyzed...

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PUM

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Abstract

The invention discloses a method for extracting vitexin and trigonelline from fenugreek. The method comprises the following steps of obtaining a vitexin-and-trigonelline-containing water extract with the yield of 20%-35% from a certain quantity of fenugreek seeds and fenugreek leaves; adding hydrochloric acid into the obtained water extract, adjusting the pH value of a solution to be 0.1-0.5, hydrolyzing for 1-2 hours, fully separating out crystals A, and re-crystallizing by adopting a 50%-90% ethanol solution to obtain crystals a; dissolving the crystals a, adding a 95% ethanol solution, separating out vitexin crystals, and spray-drying the solution in which the crystals A are separated out to obtain trigonelline. According to the method, effective traditional Chinese medicine ingredients in the fenugreek are fully dissolved in the water solution at first and then vitexin and trigonelline are extracted, so that the extraction rate of vitexin and trigonelline is greatly increased and the extraction purity of vitexin and trigonelline is greatly improved.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from traditional Chinese medicine, in particular to a method for simultaneously extracting vitexin and trigonelline from fenugreek. Background technique [0002] The chemical components contained in the herb fenugreek are trigonelline (trigonellinelline), diosgenin-B-D-glucoside (diosgenin-B-D-blucoside), vitexin (vitexin), vitexin-7-glucoside, isovitexin ( saponaretin), isoorientin (vitexin-7-glucoside), trigonelin Ⅰ, ∏ (vicenin Ⅰ, ∏) [0003] Among them, the main function of vitexin is to promote blood circulation and remove blood stasis, regulate qi and unblock the channels; it is used for chest obstruction caused by blood stasis and blockage of the veins, symptoms include chest tightness and breathlessness, tingling in the precordial area, palpitations, forgetfulness, dizziness and tinnitus. At present, vitexin is mainly used to treat cardiovascular diseases, but the purity requir...

Claims

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Application Information

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IPC IPC(8): C07D407/04C07D213/80C07D213/803
CPCC07D213/80C07D213/803C07D407/04
Inventor 刘海峰姚晨
Owner 成都合盛生物技术有限公司
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