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Application of lasiodiplodia pseudotheobromae or its fermented product in controlling wheat powdery mildew

A lasiodiplodiapseudotheobromae, 1. lasiodiplodiapseudotheobromae technology, applied in the field of microorganisms, can solve the problems of wheat powdery mildew bacteria that have not been reported in the literature, and achieve high antagonistic activity and high biocontrol activity

Inactive Publication Date: 2015-08-19
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report that Lasiodiplodia pseudotheobromae can be used as a biocontrol fungus, and there is no literature report that this strain can control wheat powdery mildew (Blumeria graminis f.sp. triti)

Method used

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  • Application of lasiodiplodia pseudotheobromae or its fermented product in controlling wheat powdery mildew
  • Application of lasiodiplodia pseudotheobromae or its fermented product in controlling wheat powdery mildew
  • Application of lasiodiplodia pseudotheobromae or its fermented product in controlling wheat powdery mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation of endophytic fungus LPS-1

[0026] Rinse the stems of the collected medicinal plant Lycidium chinensis under running water to remove the silt attached to the surface. The separation of endophytic fungi adopts the conventional tissue separation method. The sample is first washed twice with sterile water, cut into 1-2 cm long pieces, soaked in 75% alcohol for 1-2 minutes, and rinsed with sterile water for 2-3 minutes. Second-rate. Then disinfect with sodium hypochlorite with 1.5% available chlorine content for 7-10 minutes, rinse with sterile water for 3 times, cut the surface-sterilized tissue into 0.2 cm×0.2 cm fragments with a sterilized blade in a sterilized petri dish, Use sterilized filter paper to fully absorb the moisture on the surface of the sterilized tissue block. Finally, the tissue pieces were transplanted onto PDA medium (containing 50 U / mL streptomycin sulfate and ampicillin) plates and placed in a constant temperature incubator a...

Embodiment 2

[0027] Embodiment 2: endophytic fungus LPS-1 fermented liquid is to the activity measurement of wheat powdery mildew

[0028] Shake flask static fermentation method: the fresh endophytic fungus LPS-1 cultivated on the PDA was punched into a cake with a sterilized puncher with a diameter of 6 mm in the ultra-clean workbench, inoculated into a 100 mL Erlenmeyer flask and filled with 30 Milliliter malt essence medium, culture temperature 25 ℃, culture 7 days. The non-inoculated malt essence medium was used as blank control. Malt extract medium formula: malt extract 19.95g / L, sucrose 50 g / L, sodium nitrate 7.5 g / L, magnesium sulfate heptahydrate 3.561 g / L, deionized water 1L.

[0029] After fermentation, the fermentation broth was filtered and centrifuged at 11000 rpm for 10 min. Take the supernatant for later use. Using the isolated leaf segment method, the first leaf of wheat cultured to one leaf and one heart was cut into 30 mm long leaf segments, and placed on 0.5% water ...

Embodiment 3

[0034] Example 3: Observation of Morphological Characteristics of Endophytic Fungus LPS-1

[0035] The endophytic fungus LPS-1 was cultured on PDA medium at 25°C. The colonies were round, with neat edges, initially white, and gradually turned dark gray from the center of the inoculation point outward on the third day, and mature colonies were black. The aerial hyphae are developed, and the colony is fluffy, and gradually thickens into a tapetum. The hyphae are single, black, strong, long, multi-branched, without septa, and do not produce spores on PDA medium.

[0036] Induce sporulation, place sterilized pine needles on the surface of 2% water agar plate, insert endophytic fungus LPS-1 into the plate, place it in an incubator at 25°C, and cultivate under ultraviolet light and ordinary light for 12 hours alternately in light and dark In 2-5 weeks, black sporozoites (i.e. pycnidia) began to form on the pine needles. There are transparent lateral filaments in the pycnidia, cy...

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Abstract

The invention discloses an application of Lasiodiplodia pseudotheobromae or its fermentation products in the prevention and treatment of wheat powdery mildew, and relates to the technical field of wheat powdery mildew prevention and treatment. The above related endophytic fungus is separated from a medicinal plant Ilex cornuta acquired in Wuhan Botanical Garden, Chinese Academy of Sciences, is identified according to the microbial systematics, and is named Lasiodiplodia pseudotheobromae (strain code of LPS-1), the above strain is preserved in China Center for Type Culture Collection (address: Wuhan University, Wuhan, China), and the preservation number of the strain is CCTCCNO:M2013605. The strain is characterized in that a strain fermentation liquid and its ether extract can produce active products having an inhibition effect on Blumeria gromirds f.sp. tritic. The strain provides excellent original strains for the further development and exploitation of microbial source pesticides, and is an important microbial resource in the agriculture.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to the application of endophytic fungi in plants, in particular to endophytic fungi in plants Lasiodiplodia pseudotheobromae The application of the fermented product thereof in the control of wheat powdery mildew. Background technique [0002] Plant endophytic fungi refer to a class of microorganisms that live in plants or in healthy plants at certain stages of their life history, without causing any external diseases of the plants. Plant endophytic fungi are a new microbial resource, which can produce metabolites of various structural types, and have various biological activities such as antibacterial, antiviral, antitumor, anti-insect, and regulation of plant growth (Tan RX & Zou WX, 2001 ). Since endophytic fungi can produce metabolites with novel structures and unique functions, they become potential resources for screening new compounds and new drugs. Isolating endophyti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P3/00
Inventor 喻大昭向礼波龚双军杨立军张学江曾凡松薛敏峰汪华史文琦
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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