Vaccine composition and applications thereof

A vaccine composition, Duck Riemer's technology, applied in the field of vaccine composition and its application, can solve the problems of short immunization period and inapplicability to duckling immunization

Inactive Publication Date: 2014-07-09
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, considering that the susceptibility of ducklings to duck viral hepatitis is within 2 weeks of age, the immune production period of the vaccine suitable for ducklings should be shorter. However, the antibodies induced by this combination vaccine need to be produced within 3 weeks. It reached its peak in two weeks, so the double inactivated vaccine is not suitable for the immunization of ducklings

Method used

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  • Vaccine composition and applications thereof
  • Vaccine composition and applications thereof
  • Vaccine composition and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Preparation of Antigen

[0021] 1. Preparation of Serum Type Ⅰ Duck Hepatitis Virus Antigen

[0022] Take the attenuated duck hepatitis virus strain A66 of serum type Ⅰ, and use the published method (Zhang Xiaofei, Development of duck viral hepatitis attenuated vaccine (A66 strain) [D]. Nanjing: Nanjing Agricultural University, 2010), inoculate SPF chicken embryos Breeding was carried out, and the infected chick embryo fluid was harvested. The virus content of the infected chicken embryo fluid was determined to be 10 8.5 ELD 50 / 0.1ml, take part of the virus solution and dilute it to 10 4.0 ELD 50 / 0.1ml and 10 2.5 ELD 50 5% sucrose skim milk and vacuum freeze-dried to make serum type Ⅰ duck hepatitis virus antigens with different virus contents. The batch numbers are D1201, D1202 and D1203 respectively.

[0023] 2. Preparation of serotype 1 Riemerella anatipestifer antigen

[0024] Take the serotype 1 Riemerella anatipestifer Wuhan isolate, and use ...

Embodiment 2

[0025] Example 2. Preparation of vaccine

[0026] The prepared 3 batches of serum type 1 duck hepatitis virus antigen and 3 batches of serum type 1 Riemerella anatipestifer antigen were mixed separately as a vaccine composition (hereinafter referred to as "vaccine composition"), and 3 batches of vaccine combinations with different antigen contents were prepared. The batch numbers are 1201, 1202 and 1203 respectively, and each dose (0.3ml) of the mixed vaccine composition contains 1 dose (the content of the antigen is 10 2.5 ELD 50 / 0.1ml, 10 4.0 ELD 50 / 0.1ml and 10 8.5 ELD 50 / 0.1ml) serum type Ⅰ duck hepatitis virus antigen and 1 dosage (the content of the antigen before inactivation is 10 7.0 CFU / ml, 10 9.0 CFU / ml and 10 12.0 CFU / ml) of serotype 1 Riemerella anatipestifer antigen. The specific data of the vaccine composition is shown in Table 1.

[0027] The batch number and antigen content of the antigen used in the vaccine composition of table 13

[0028]

...

Embodiment 3

[0030] Application in embodiment 3. breeding ducks

[0031]Take the 3 batches of vaccine compositions and control vaccines prepared in Example 2, and administer them once at the age of 110 days and 130 days in shelduck ducks (purchased from Luoyang Fumin Poultry Breeding Co., Ltd.) with one dose (0.3ml) respectively. Inject immunization, collect eggs hatched 20-30 days after the second injection, and hatch ducklings. When the ducklings were 1 week old, 2 weeks old and 3 weeks old, some ducklings were taken to test the efficacy of serum type 1 duck hepatitis virus and serum type 1 Riemerella anatipestifer. As a control, ducklings hatched from shelduck ducks without any immunization were used as control animals. The results are shown in Table 2.

[0032] The duration of passive immunity of ducklings hatched after table 2 adult female duck immunization

[0033]

[0034] Note: 1 The numerator represents the number of surviving ducks, and the denominator represents the tot...

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Abstract

The present invention relates to a vaccine composition, which contains inactivated riemerella anatipestifer antigen and live duck hepatitis virus antigen. The invention further provides application of the vaccine composition in preparation of products for concurrent prevention or treatment of breeding duck and/or young duck viral hepatitis and infectious serositis of ducks, and in preparation of products for increasing growth performances of young ducks. According to the present invention, the one-time immunization is adopted, such that the number of the vaccine injection can be reduced, the stress reaction of the young ducks can be reduced, and the impact on the young duck growth can be reduced; and the inactivated riemerella anatipestifer antigen and the live duck hepatitis virus antigen are adopted to combine so as to shorten the immunization production period of the duck hepatitis virus antigens, such that the vaccine composition is suitable for young duck immunization.

Description

technical field [0001] The invention relates to a vaccine composition for preventing or treating duck viral hepatitis and duck infectious serositis and its application. Background technique [0002] Duck viral hepatitis and duck infectious serositis are one of the two main infectious diseases of ducklings. The two diseases mainly affect ducklings within 8 weeks of age, especially 2-3 week-old ducklings. In individual cases, adult ducks and breeding ducks also occur. In actual production, vaccination is usually required for the prevention of poultry diseases. However, every injection of the vaccine will cause stress to the poultry, thereby affecting the growth and reproduction performance of the poultry. For example, if poultry are vaccinated during egg laying, the stress response may reduce the egg production of the poultry; for another example, if young poultry are infected with chronic respiratory diseases, if eye drops or nasal drops are used for vaccination, the stress ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/04A61K39/29A61K39/02
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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