Application of Cystatin S and CYFRA21-1 (Cytokeratin Fragement 21-1) in preparation of markers for diagnosing and predicting esophagus cancer

A marker, esophageal cancer technology, applied in the field of medical detection, can solve problems such as differential survival rate, and achieve the effect of improving sensitivity and specificity, easy to carry, and convenient to use

Active Publication Date: 2014-07-02
SHANGHAI LIANGRUN BIOMEDICINE TECH CO LTD
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, clinically, even patients at the same pathological stage show significantly different survival rates

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Cystatin S and CYFRA21-1 (Cytokeratin Fragement 21-1) in preparation of markers for diagnosing and predicting esophagus cancer
  • Application of Cystatin S and CYFRA21-1 (Cytokeratin Fragement 21-1) in preparation of markers for diagnosing and predicting esophagus cancer
  • Application of Cystatin S and CYFRA21-1 (Cytokeratin Fragement 21-1) in preparation of markers for diagnosing and predicting esophagus cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Establishment of Cystatin S Serum Detection Reaction System and Its Optimization

[0026] Coat the ELISA plate with a mouse anti-human Cystatin S monoclonal antibody at a concentration of 5 μg / mL. The coating condition is to coat overnight at 4°C, wash the plate, and then block it in 2% BSA at room temperature for 2 hours. After washing the plate, the Cystatin S protein standard substance (the amino acid sequence of encoding Cystatin S such as SEQ ID NO.1) and samples were added to the closed plate, reacted at 37°C for 1 hour, and washed the plate; then reacted with rabbit anti-human Cystatin S polyclonal antibody labeled with HRP at a concentration of 0.5 μg / mL at 37°C for 1 hour, Wash the plate; react with tetramethylbenzidine (TMB) for 2-3 minutes, and finally stop the reaction with 2M sulfuric acid, and detect the OD value at 450nm ( figure 1 ). Depend on figure 1 It can be seen that the linear range of the Cystatin S enzyme-linked immunoassay kit is 50...

Embodiment 2

[0033] Embodiment 2, Cystatin S ELISA kit

[0034] According to the Cystatin S serum detection system established in Example 3, the Cystatin S enzyme-linked immunoassay kit was constructed, and the specific components are as shown in Table 1:

[0035] Table 1. Cystatin S ELISA Kit Components

[0036]

[0037]

[0038] Evaluate the Cystatin S ELISA kit: Use the Cystatin S ELISA kit to detect the Cystatin S positive quality control product, and repeat the detection 10 times at the Cystatin S protein concentration of 160pg / mL and 80pg / mL respectively. It shows that the coefficient of variation CV≤10%; the same sample is tested with 3 batches of kits, and the inter-assay coefficient of variation CV of 3 batches of kits is ≤15%. The research on the stability of the kit shows that it can be kept stable after being stored at 4°C for 8 months, stored at 4°C for 2 months after opening, and transported at 0-4°C for 7 days.

Embodiment 3

[0039] Example 3 Establishment of CYFRA21-1 Serum Detection Reaction System

[0040] The CYFRA21-1 enzyme-linked immunosorbent assay (ELISA) detection kit used in this example was a commercial CYFRA21-1 ELISA kit purchased from Beijing Huamaike Biotechnology Co., Ltd.

[0041] The CYFRA21-1 ELISA kit and the Cystatin S ELISA kit constructed in Example 2 were used to form a Cystatin S-CYFRA21-1 combined detection kit for the detection of esophageal cancer markers CYFRA21-1 and Cystatin S , wherein the amino acid sequence of the tested marker CYFRA21-1 is shown in SEQ ID NO.2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to view more

Abstract

The invention discloses combined application of cysteine proteinase inhibitor S (Cystatin S) and cytokeratin-19-fragment 21-1 (CYFRA21-1) and in particular discloses application of Cystatin S and CYFRA21-1 in preparation of markers for diagnosing and predicting esophagus cancer. The invention also discloses a trapping agent of esophagus cancer markers and a kit comprising the trapping agent. The kit has the advantages of good specificity and high sensitivity and can be applied to early diagnosis of the esophagus cancer, therapeutic effect evaluation in a breast cancer treating process and monitoring of transfer and recurrence after treating the breast cancer; diagnosis results can be earlier than clinical symptoms.

Description

technical field [0001] The invention belongs to the field of medical detection, and relates to the application of Cystatin S and CYFRA21-1 in preparing markers for diagnosing and predicting esophageal cancer. Background technique [0002] Esophageal cancer is one of the most common malignant tumors in the world. my country is the country with the highest incidence and mortality rate of esophageal cancer in the world, and its mortality rate ranks fourth in the national malignant tumor mortality rate. The symptoms of early esophageal cancer are not obvious, and most patients with esophageal cancer are already in the middle and late stages when they are diagnosed. After surgical treatment of early esophageal cancer, the 5-year survival rate can reach more than 80%. Therefore, early detection and early treatment of esophageal cancer is the best way to improve the survival rate of patients with esophageal cancer. [0003] Currently, the diagnosis of esophageal cancer mainly re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577
CPCG01N33/57407G01N33/57484G01N2333/4742G01N2333/8139G01N2800/52G01N2800/54
Inventor 王弢秦勇渠香云
Owner SHANGHAI LIANGRUN BIOMEDICINE TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap