Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of paraffin section for microscopic observation from cashmere goat skin

A paraffin section and microscopic observation technology, applied in the field of animal histology, can solve the problems of lack of skin, no detailed description of the production process of skin paraffin section, high degree of keratinization, and achieve the phenomenon of not easy fragmentation, clear picture structure, and process flow. simple effect

Inactive Publication Date: 2014-07-02
GANSU AGRI UNIV
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of solid tissue, the skin is highly keratinized and relatively brittle, which poses a great challenge during slicing
However, there is no detailed description of the skin paraffin section making process in the relevant literature.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of paraffin section for microscopic observation from cashmere goat skin
  • Preparation method of paraffin section for microscopic observation from cashmere goat skin
  • Preparation method of paraffin section for microscopic observation from cashmere goat skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: A kind of cashmere goat skin is used for the preparation method of microscopic observation paraffin section, and its main feature is to comprise steps to be:

[0025] (1) Take a skin sample; select an adult cashmere goat, and cut a 0.8cm from the side of the body with scissors 2 skin sample;

[0026] (2) Fixation of the sample: flatten the cut skin sample on the prepared glass slide, and put it in a container with 4% paraformaldehyde fixative solution, 2°C, 12h;

[0027] (3) Softening of skin samples: Put the fixed skin samples into softening solution to soften, room temperature, 48h;

[0028] (4) Dehydration of skin samples: at 18°C ​​in sequence, 70% alcohol for 5h, 80% alcohol for 12h, first time for 95% alcohol for 2h, second time for 95% alcohol for 1h50min, first time for 100% alcohol for 2h, second time Secondary 100% alcohol 1h50min, 1:1 alcohol xylene 10min, xylene 6min30s;

[0029] (5) Paraffin treatment: in an oven at 65-70°C, put in 1:1 para...

Embodiment 2

[0033] Embodiment 2: A kind of cashmere goat skin is used for the preparation method of microscopic observation paraffin section, comprises steps and is:

[0034] (1) Take a skin sample; select an adult cashmere goat, and use scissors to cut 1.2cm from the side of the body 2 skin sample;

[0035] (2) Fixation of the sample: flatten the cut skin sample on the pre-prepared glass slide, and put it in a container with 4% paraformaldehyde fixative solution for fixation, 4°C, 24h;

[0036] (3) Softening of skin samples: Put the fixed skin samples into softening solution to soften, room temperature, 48h;

[0037] (4) Dehydration of skin samples: at 22°C in sequence, 70% alcohol for 6h, 80% alcohol for 12h, first time for 95% alcohol for 2h, second time for 95% alcohol for 1h50min, first time for 100% alcohol for 2h, second time Secondary 100% alcohol 1h50min, 1:1 alcohol xylene 10min, xylene 6min30s;

[0038] (5) Paraffin treatment: proceed in sequence in an oven at 70°C, put in 1...

Embodiment 3

[0042] Embodiment 3: A kind of cashmere goat skin is used for the preparation method of microscopic observation paraffin section, comprises steps and is:

[0043] Steps (1) to (7) are the same as those in Embodiment 1 or Embodiment 2.

[0044] (8) HE staining of skin samples: Dewax with xylene twice, each time for 10 minutes; then use 100% alcohol for 10 minutes for the first time; use 100% alcohol for the second time for 5 minutes; 5min; 80% alcohol 5min; 70% alcohol 5min; distilled water 3min; hematoxylin (Delafield) 10min; distilled water wash 1min; 1% hydrochloric acid alcohol 5S; 70% alcohol for 5 s; 80% alcohol for 5 s; 95% alcohol twice, 5 min each; 100% alcohol twice, 10 min each; xylene twice, 10 min each, and finally routinely sealed.

[0045] Delafield hematoxylin preparation method used: hematoxylin 4g; absolute ethanol 25ml; 10% ammonium alum aqueous solution 400ml; glycerin 100ml; methanol 100ml.

[0046] The preparation method of 1% water-soluble eosin used (g...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to auxiliary researches of protein localization in the technical field of animal histology, particularly a preparation and dyeing method of a paraffin section for microscopic observation. The preparation method is mainly characterized by comprising the following steps: taking a skin sample; fixing the sample; softening the skin sample; dehydrating the skin sample; carrying out paraffin treatment; carrying out conventional embedment on the skin sample; and sectioning the skin sample. The method is simple in process and convenient to operate; and the obtained section is complete and has clear picture structure. The method is suitable for laboratory researchers to research the morphological structure of cashmere goat skin. When being observed under a light microscope, the paraffin section can completely display the epidermis and derma structures of the skin, does not have wrinkle or crushing, and can not easily have the phenomenon of crushing in the sectioning process.

Description

technical field [0001] The invention relates to the auxiliary research of protein localization in the technical field of animal histology, in particular to the preparation and staining method of paraffin sections for microscopic observation. Background technique [0002] Longdong cashmere goats are a new group bred by Liaoning cashmere goats and Inner Mongolia cashmere goats and local goats, and are preparing to apply for national identification and acceptance. According to the measurement statistics, the average cashmere production of adult rams is 578g; the average cashmere production of adult ewes is 451g; the slaughter rate is 47.08%. It is mainly distributed in Huan County, Huachi County and Qingcheng County of Gansu Province. The cashmere goat industry is a local pillar industry, and cashmere is the main product of cashmere goats. Therefore, the research on cashmere goat skin has become a research hotspot for a time. From the structural point of view, the preparatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/28
Inventor 贺延玉刘秀孟云
Owner GANSU AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com