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Clostridium beijerinckii with high electricity yield and application thereof

A technology of Clostridium beijerinckii and Clostridium beijerinjecki, which is applied in the application field of carbon source in microbial fuel cell power generation, can solve the problems such as few reports of Clostridium beijerinjecki, achieve high electron recovery and utilization rate, and stress resistance strong effect

Inactive Publication Date: 2014-07-02
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Juliane Niessen et al. (Electrochemistry Communications6(2004) 955–958) performed MFC electricity production on Clostridium butyricum, using 10g / L glucose as fuel to obtain a maximum output voltage of 473mV. However, detailed reports on MFC electricity production by Clostridium beijerinckii little

Method used

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  • Clostridium beijerinckii with high electricity yield and application thereof
  • Clostridium beijerinckii with high electricity yield and application thereof
  • Clostridium beijerinckii with high electricity yield and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] This example illustrates the first step of plasma mutagenesis of the original strain of Clostridium beijerinckii.

[0055] The method for the first step plasma mutagenesis of the original bacterial strain of Clostridium beijerinckii is as follows:

[0056] The original strain of Clostridium beijerinckii NCIMB8052 was activated and cultured at a culture temperature of 33-37°C, the volume of 50ml Schott anaerobic bottle was 15-20ml, filled with nitrogen for 3 minutes, and the culture time was 12-18 hours to obtain a vigorously growing bacterial liquid; take fresh Cultured cells were diluted to cell concentration OD 600 =1~1.5, drop on the sterilized and cooled slide, dry with sterile air; use helium as the discharge gas, 100W as the radio frequency power, 10SLM as the gas flow rate, and 10~1800s as the irradiation time Plasma mutagenesis was performed on the bacterial strain, and after the mutagenesis, the bacterial film on the carrier was eluted, and the survival rate w...

Embodiment 2

[0058] This example illustrates the method for screening out high electricity producing Clostridium beijerinckii.

[0059] Wherein, the culture medium formula used is as follows:

[0060] (1) Seed medium: yeast powder 3g / L, peptone 5g / L, soluble starch 10g / L, ammonium acetate 2g / L, sodium chloride 3g / L, magnesium sulfate heptahydrate 3g / L, potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, ferrous sulfate heptahydrate 0.1g / L, pH=6.

[0061] (2) The sandwich medium is as follows:

[0062] a), solid medium: yeast powder 3g / L, peptone 5g / L, soluble starch 10g / L, ammonium acetate 2g / L, sodium chloride 3g / L, magnesium sulfate heptahydrate 3g / L, potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, ferrous sulfate heptahydrate 0.1g / L, methyl viologen 0.10g / L, pH=6 agar 15~20g / L.

[0063] b), WO 3 Agar suspension: WO 3 Clusters 5g / L, sodium chloride 10g / L, agar 20g / L, methyl viologen 0.10g / L.

[0064] (3) Sodium lactate minimum salt ...

Embodiment 3

[0082] This example illustrates the electricity production experiment using 1 g / L glucose by Clostridium beijerinckii as an anode catalyst.

[0083] (1) Construction of microbial fuel cells

[0084] In this embodiment, a microbial fuel cell using Clostridium beijerinckii to generate electricity as an anode catalyst is established according to existing technologies and methods, as shown in the attached figure 2 As shown, it includes four parts: anode chamber, cathode chamber, proton exchange membrane and external circuit. The anode electrode and cathode electrode are both PAN-based graphite soft felt (5×5cm), titanium wire is used as the external circuit, the external resistance is 1000Ω, the proton exchange membrane is DuPont Nafion N117, and the data collector is Keithley series.

[0085] (2) The highly electrogenic strain M13 and the initial strain 8052 obtained from the screening were activated in the seed solution, the culture temperature was 37°C, and the anaerobic cult...

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Abstract

The invention discloses clostridium beijerinckii with high electricity yield. The number of the strain is M13. Clostridium beijerinckii is collected in the China Center for Type Culture Collection (CCTCC) on September 14, 2013, with collection number of CCTCC NO:M2013424. The invention also discloses an application of clostridium beijerinckii with high electricity yield to electricity generation. When 0.15g / L of methyl viologen serves as an electronic mediator, the maximum output voltage of clostridium beijerinckii in 1g / L of glucose seed solution is as high as 230mV, and the maximum output power density is 79.2mW / m<2>. Clostridium beijerinckii has the advantages that a process is simple, is convenient to operate, is pollution-free and is low in cost; utilization of fuel of clostridium beijerinckii is popularized more widely; the electron recovery rate is relatively high; the energy crisis is relieved; clostridium beijerinckii is an excellent strain suitable for microbial fuel cell study and application.

Description

technical field [0001] The invention belongs to the technical field of environment and new energy, and specifically relates to a Clostridium beijerinckii and its application in utilizing carbon sources such as glucose to generate electricity in microbial fuel cells. Background technique [0002] Energy depletion and the disposal of organic waste are two major problems facing the world today. However, with the progress and development of human beings, the demand for energy and the problem of global warming are becoming increasingly severe. In order to solve these problems, scientists have turned their attention to microorganisms, using microorganisms to generate electricity to solve the problem of energy shortage; at the same time, using the ability of microorganisms to degrade surrounding organic matter for energy to maintain growth, to deal with the growing organic waste problem. As a result, the importance of microbial fuel cells (MFCs) has become increasingly apparent. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20H01M8/16C12R1/145
CPCY02E60/527Y02E60/50Y02P70/50
Inventor 应汉杰刘俊郭亭王冬华莹谢婧婧陈勇吴菁兰陈晓春朱晨杰庄伟
Owner NANJING UNIV OF TECH
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