Preparation method of methylamphetamine-resistant egg yolk antibody
A methamphetamine and egg yolk antibody technology, applied in chemical instruments and methods, from egg immunoglobulins, peptides, etc., can solve the problem of lack of specific treatment drugs for methamphetamine, and reduce addiction and relapse behavior , low cost, and the effect of reducing toxicity
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[0034] One, the preparation of methamphetamine-bovine serum albumin antigen
[0035] 1 Synthesis and identification of hapten
[0036] The hapten can be combined with the corresponding antibody to form an antigen-antibody, and the hapten cannot alone stimulate the human or animal body to produce antibodies.
[0037] 1.1 Synthesis
[0038] Weigh 150mg of methamphetamine hydrochloride and dissolve it in 150mg of distilled water, add dropwise a NaOH solution with a concentration of 1mol / L until the free form of methamphetamine is separated out, extract with chloroform (analytical grade), and extract the product with NaOH 2 SO 4drying the separated methamphetamine, filtering the separated methamphetamine, and evaporating the solvent to obtain the methamphetamine. Dissolve 120mg of methamphetamine in 120ml of absolute ethanol (spectrum pure), then add 100ml of 1mol / L N-(4 bromobutyl)phthalimide-absolute ethanol solution, and add 1-5ml appropriately Triethylamine helps to dissol...
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