Method for rapidly determining polysaccharide peptide in lucid ganoderma product

Abstract

The invention provides a method for rapidly determining polysaccharide peptide in a lucid ganoderma product. The method takes a high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD) as a detection means and takes lucid ganoderma polysaccharide peptide as a comparison product to rapidly detect the content of the polysaccharide peptide in the lucid ganoderma product. The pre-treatment of a sample only needs simple extraction operation and is convenient and rapid; finally, after the sample is subjected to chromatography high-performance separation, the evaporative light-scattering detector is used for carrying out quantitative analysis on the polysaccharide peptide in the product; a determination result is high in accuracy and good in stability; the method is better than a current common spectrophotometric method, so as to provide a very effective analyzing method for quality identification and quality control of the lucid ganoderma product.

Description

technical field [0001] The invention belongs to the field of analysis of traditional Chinese medicinal materials, and in particular relates to a method for rapidly determining polysaccharide peptides in ganoderma lucidum products by using an HPLC-ELSD method. Background technique [0002] Ganoderma lucidum is a fungus of the genus Ganoderma of the basidiomycete polyporaceae family. It is a treasure that nourishes and strengthens the body and strengthens the body. It has a long history of medicinal use. The application of modern science and technology makes the study of Ganoderma lucidum more in-depth, and further proves that Ganoderma lucidum and its active ingredients have a wide range of pharmacological effects, which can prevent chronic bronchitis, neurasthenia, hyperlipidemia, coronary heart disease, hepatitis, leukopenia, tumors and other diseases . Ganoderma lucidum polysaccharide peptide, as one of the active ingredients of Ganoderma lucidum, is a variety of glycopep...

AI Technical Summary

Problems solved by technology

At present, the only detection method for polysaccharide peptides is the FoLin-phenol method reported by Chen Ying et al. (Chen Ying, Wang Saizhen, Lin Shuqian. Determination of polysaccharide peptide content in Ganoderma lucidum products by FoLin-phenol method[J]. Strait Pharmacy, 2007, 19 (7): 37-39.), the specific operation is to add 1.0 mL of alkaline copper test solution to 1 mL of sample, place it at room temperature for 10 minutes, add 4 mL of FoLin-phenol test solution, bathe in 55 ° C for 5 minutes, cool to room temperature, and measure at 750 nm Absorbance value, but the reagent preparation of this method is cumbersome, and the detection result is not stable enough

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