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Solid culture medium and culture method of beauveria

A solid medium and culture method technology, applied in the field of fermentation culture, can solve the problems of unstable product quality, long culture cycle, high cost, etc., and achieve the effects of rapid proliferation, low production cost, and short production cycle

Inactive Publication Date: 2014-06-25
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a solid culture medium of Beauveria bassiana and a method for cultivating Beauveria bassiana, aiming to solve the problems of long culture period, low output, unstable product quality and high cost in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Preparation of solid medium for Beauveria bassiana:

[0016] It is obtained by steam sterilizing 5g of vermiculite, 5-6g of wheat bran and 5-6g of corn flour for 40 minutes.

[0017] (2) Preparation of liquid expansion medium:

[0018] 8g glucose, 2g peptone, 2g yeast extract, 0.2gNaH 2 PO 4 , 0.1gMgSO 4 , 0.1gKCl and 200mlH 2 After O mixing, the mixture obtained was steam sterilized for 40 minutes, and after cooling, 0.1% gentamycin sulfate was added to the mixture.

Embodiment 2

[0020] A method for cultivating Beauveria bassiana, comprising the following steps:

[0021] (1) Add 5ml to a concentration of 10 7 The Beauveria bassiana inoculum of spores / mL was cultured in the 150ml liquid expansion medium in Example 1 at 27±1°C, 220~250r / min and shaken for 72h;

[0022] (2) Insert the solid medium in Example 1, the solid medium is placed in a sterilized canned bottle sealed with sterilized newspaper, each bottle of solid medium is 15g, and the inoculated amount is 10-15ml / 100g, The amount of sterilized water is 50%-55%, the canned bottle is put into 8# zipper bag and sealed, and it is harvested after culturing at 25-27°C for 7.5-8 days.

[0023] (3) The harvested product was measured, and the amount of conidia produced before drying reached 3.7×10 9 Spores / g, after drying, the unit spore production is more than 10 billion per gram.

[0024] Compared with the shortcomings and deficiencies of the prior art, the present invention has the following benefic...

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Abstract

The invention provides a solid culture medium and culture method of beauveria. The solid culture medium comprises vermiculite, wheat bran and corn flour in a mass ratio of 1:(1-1.2):(1-1.2), wherein vermiculite, wheat bran and corn flour are mixed and sterilized with steam for 40 minutes, thus obtaining the solid culture medium. The culture method comprises the steps of culturing beauveria inoculation fluid in a liquid enlarge culture medium to which gentamicin sulphate is added, then inoculating the beauveria inoculation fluid into the solid culture medium, and culturing the beauveria inoculation fluid at 25-27 DEG C for 7.5-8 days, thus harvesting beauveria. The solid culture medium has good moisture retention and breathability, can provide rich N and C source nutrients for beauveria proliferation, and is economical and accessible in raw materials. The culture method is simple to operate, is clear in flow, has low requirements for production environments and production equipment, is relatively low in production cost and relatively short in production period, dispenses with discharging the culture medium in production so as to completely eradicate the problem of pollution of mixed fungi in production, and is very suitable for small enterprises and production units to quickly produce beauveria.

Description

technical field [0001] The invention belongs to the technical field of fermentation and cultivation, and in particular relates to a solid culture medium of Beauveria bassiana and a method for cultivating Beauveria bassiana. Background technique [0002] Beauveria bassiana is a common entomopathogenic fungus with broad-spectrum insecticidal properties. It has a wide range of hosts, strong pathogenicity, non-toxic to humans, animals and plants, does not kill natural enemies, does not pollute the environment, and has no chemical pesticide residues , easy to cultivate, and competitive cost of control. It is the most widely used pathogenic fungus for biological control of pests at home and abroad. It has been successfully used in the biological control of dozens of major agricultural and forestry pests at home and abroad, and has developed many A Beauveria bassiana biological agent is used for commercial production, and Beauveria bassiana is currently the most widely used biologi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 赵奎军于洪春宋龙腾戴长春裴海英
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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