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Liquid and method for screening hepialus armoricanus eggs

A technology of bat moth eggs and screening methods, applied in botany equipment and methods, horticulture, application, etc., can solve the problem that there is no effective classification of bat moth eggs, and achieve the effect of increasing hatching rate and increasing hatching rate

Active Publication Date: 2014-06-25
YICHANG SHANCHENGSHUIDU CORDYCEPS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the effective classification of bat moth eggs according to their development status.

Method used

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  • Liquid and method for screening hepialus armoricanus eggs
  • Liquid and method for screening hepialus armoricanus eggs
  • Liquid and method for screening hepialus armoricanus eggs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Sucrose phosphate buffer solution to detect egg effect:

[0048] Get the first batch of bat moth eggs, get 3 portions of eggs altogether, each portion of 100 grains.

[0049] Two copies of eggs A1-1 and A1-2 were taken and dissected to check the development status. The results are shown in Table 1 below.

[0050] For the other 100 eggs A2, adjust the sucrose content in the screening solution to 1%, 10%, 15% and 20%, take 5mL of the solution respectively, and transfer it into a 10mL centrifuge tube. The bat moth eggs were sampled as a whole and immersed in the test solutions of several sucrose concentrations successively from low to high. Put the eggs into each centrifuge tube, turn it upside down 6 to 8 times, and wait for it to sink. Eggs on the floating liquid surface of each treatment are taken out and placed in a Petri dish covered with filter paper marked with the corresponding mark, the filter paper is humidified, and then the eggs that sink to the bottom are pu...

Embodiment 2

[0116] The effect of adding taurine to sucrose phosphate buffer solution to detect eggs:

[0117] Get the same bat moth egg, a total of 4 eggs, each 100.

[0118] Set 15% sucrose 0.1mol / L phosphate buffer (PH=7) with concentration gradient between 0.2~1mmol / L taurine.

[0119] After each group of eggs were treated in phosphate buffered saline with different molar concentrations, the eggs were placed in the rearing substrate for rearing, and the number of larvae was counted in the substrate after 30 days.

[0120] The experimental results are shown in Table 10.

[0121] Table 10 The late effect of taurine with different molar concentrations and sucrose phosphate buffer compatibility on bat moth eggs

[0122]

[0123] According to the test results, adding 0.2-1.0mmol / L taurine to sucrose phosphate buffer solution, the hatching rate and survival rate of larvae are higher than those without adding taurine.

Embodiment 3

[0124] The preparation of embodiment 3 bat moth ovum screening liquid

[0125] Configure 1mol / L anhydrous sodium dihydrogen phosphate (NaH 2 PO 4 ) stock solution: dissolve 120g in sufficient water to make the final volume 1L;

[0126] 1mol / L disodium hydrogen phosphate (Na 2 HPO 4 ) stock solution: dissolve 142g in sufficient water to make the final volume 1L;

[0127] Take 1mol / L anhydrous sodium dihydrogen phosphate (NaH 2 PO 4 ) stock solution 877ml and 1mol / L disodium hydrogen phosphate (Na 2 HPO 4 ) were mixed with 123ml of the stock solution so that the pH of the mixed solution was 6.

[0128] After adding 3125g sucrose, add water to dilute 25 times to obtain a sucrose phosphate buffer solution with a salt concentration of 0.04mol / L (mass concentration of sucrose is 12.5%).

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Abstract

The invention relates to the biotechnical field, in particular to liquid and a method for screening hepialus armoricanus eggs. The liquid for screening the hepialus armoricanus eggs comprises cane sugar and buffer liquid. A layering effect can be realized by the cane sugar for the hepialus armoricanus eggs which develop to different degrees; a pH (potential of hydrogen) value can be effectively regulated by the buffer liquid, and a hepialus armoricanus hatching rate further can be increased by the aid of salt concentration. The liquid and the method for screening the hepialus armoricanus eggs have the advantages that quality detection can be quickly carried out on the development degrees of the hepialus armoricanus eggs by the aid of cheap and easily available materials and the simple and convenient method, the experimental hepialus armoricanus eggs can be optimized without being destroyed, and accordingly a hatching rate of screened serviceable eggs and a survival rate of hatched hepialus armoricanus can be increased.

Description

[0001] This application claims the priority of the Chinese patent application submitted to the China Patent Office on December 19, 2012, the application number is 201210554851.1, and the title of the invention is "a kind of bat moth egg screening liquid and bat moth egg screening method", the entire content of which Incorporated in this application by reference. technical field [0002] The invention relates to the field of biotechnology, in particular to a screening solution for bat moth eggs and a screening method for bat moth eggs. Background technique [0003] Cordyceps sinensis is a famous and precious Chinese herbal medicine in China, and it is listed as the three major Chinese tonics together with ginseng and velvet antler. As early as 1757, in "Materia Medica Congxin", there is a record that "Cordyceps sinensis is sweet and flat, protects the lungs, nourishes the kidneys, nourishes the essence, stops bleeding and resolves phlegm, and is good for treating diaphragmati...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G1/04
Inventor 李全平李文佳华献春蒋均才谢俊杰贺媛蒋成吉
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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