A multi-channel independent control integrated microfluidic chip based on thin-film acoustic wave device

Abstract

The invention discloses a multi-channel independent control integrated micro-fluidic chip based on a thin film acoustic wave device. The multi-channel independent control integrated micro-fluidic chip comprises an upper-layer cover sheet and a lower-layer substrate, wherein the upper-layer cover sheet is integrated with a sample chamber, a liquid storage tank, a fixing groove of a thin film acoustic wave sensor, a micro-valve switch structure and a micro-column positioning structure; microchannels are molded on the substrate; the micro-valve switch structure is composed of a valve film and a valve cavity; the valve film is pressed by a micro-valve switch fixing device and the micro-valve switch fixing device is accurately positioned by the micro-column positioning structure. With the adoption of the plurality of micro-channels, a plurality of markers in small-type samples are subjected to high flux determination; each micro-channel independently controls a micro-valve switch to obtain detection results of the sample under different use amounts and different determination time; the detection precision of pg / mL is realized by using the thin film acoustic wave sensor; the washing of a system can be automatically carried out so that the multi-channel independent control integrated micro-fluidic chip is repeatedly utilized and the problems of the integrated micro-fluidic chip that the process is complicated and the cost is high are effectively solved.

Description

technical field [0001] The invention belongs to the field of microfluidic chips, in particular to a multi-channel independent control integrated microfluidic chip based on a thin film acoustic wave device. Background technique [0002] During the occurrence of tumors, the increase of certain tumor markers in serum is closely related to tumors. Therefore, the detection of tumor markers plays an important role in the screening, diagnosis and prognosis of tumors. In order to achieve early diagnosis and treatment of tumors, most of the current research focuses on the detection of a tumor marker in living cells, which may bring "false positive" results. Because some tumor markers are expressed not only in cancer cells, but also in normal cells, it is of great significance to simultaneously detect multiple tumor markers in living cells. For example, liver cancer usually requires simultaneous detection of carcinoembryonic antigen (CEA), carbohydrate antigen 19 (CA19-9), alpha-fet...

AI Technical Summary

Problems solved by technology

However, the integrated system is extremely easy to cause pollution. After each measurement is completed, the sample chamber and the microchannel system must be cleaned, especially for the testing of multiple markers. The process is very cumbersome

The use of disposable polymer microfluidic chips is a common practice at present, but with the existing micro-processing technology, the process of processing an integrated microfluidic chip is relatively complicated and the cost is relatively high, and the cost of disposable chips is relatively high.

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