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High-activity and high-content superoxide dismutase (SOD) roxburgh rose composite tablet preparation method

A superoxide, high activity technology, applied in food preparation, food science, application, etc., can solve the problems of superoxide dismutase loss, long drying time, etc., achieve high onset time, solve storage difficulties, high good content effect

Inactive Publication Date: 2014-06-11
李良奎
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional production process requires sun-drying and drying processing, because the drying time is long, and when drying, the ultraviolet rays in the sun will cause a large amount of superoxide dismutase (SOD) to be lost in the prickly pear
And in some improved processes, although through steps such as pulverization, thermal shock, centrifugation, also can cause the problem of the loss of a large amount of superoxide dismutase (SOD) in Rosa roxburghii because of the existence of high temperature link

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0015] 1. Material selection: choose fresh prickly pears without insects, moths, and mildew. After removing impurities, first wash them with 1-2% sodium chloride solution for 2-3 times, and then use them at a temperature lower than 30°C. Soak in deionized water for 48 hours, then dry;

[0016] 2. Chopping and refining: cut the obtained prickly pear into pieces, add 200umol / L phosphate buffer solution at 10-30L / kg, adjust the pH value in the range of 7-9, and then grind, after coarse grinding , after refined grinding, get the prickly pear puree;

[0017] 3. Enzymolysis: Add enzymes (cellulase, hemicellulase, pectinase) to the ground Rosa roxburghii puree, mix well, and then add chlorine with a concentration of 10-40% at 100-200mL / kg Calcium solution, let stand for no less than 3 hours for precipitation;

[0018] 4. Use a high-speed tubular separator at 5000rpm-10000rpm to centrifuge for 10-20 minutes to obtain the crude liquid, and keep the supernatant;

[0019] 5. Ultrafilt...

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Abstract

The invention provides a preparation method for a high-activity and high-content superoxide dismutase (SOD) roxburgh rose composite tablet preparation method. Wild roxburgh roses are used as a raw material, and superoxide dismutase (SOD) is extracted by a whole-course low-temperature technology; after making is executed, a thin film coating technology is adopted to avoid air contact, so that the content and the activity of the SOD are retained to the maximum extent; the activity preservation period of the SOD can be prolonged during tablet making. The method comprises the steps of material selection, pulping, enzymolysis, rough filtration, ultrafiltration, granulation and tabletting. The preparation method has the characteristics of simplicity, quickness, high activity, short response time, high content and good effect; the problems of difficulty in storage of the SOD and loss of effective components of a finished product are solved.

Description

technical field [0001] The invention belongs to the extraction and preparation technology of bioactive active ingredients, and in particular relates to a method for extracting superoxide dismutase (SOD) in a low-temperature environment and making tablets by using wild roxburghii fruit as a raw material. Background technique [0002] Superoxide dismutase (SOD) has its special biological activity. It adapts to a pH value of 5-10, and the activity is the highest at pH = 8. It has the most stable biological activity when the ambient temperature is below 60°C. The traditional preparation process requires sun-drying and drying processing, because the drying time is long, and when drying, the ultraviolet rays in the sun will cause a large amount of superoxide dismutase (SOD) in Rosa roxburghii to be lost. And in some improved processes, although through steps such as pulverization, heat shock, centrifugation, also can cause the problem of a large amount of superoxide dismutase (SOD...

Claims

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Application Information

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IPC IPC(8): A23L1/212A23L1/29A23L19/00A23L33/00
CPCA23L33/00A23L19/01A23L33/15A23L33/16A23V2002/00
Inventor 李良奎
Owner 李良奎
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