Preparation method and use of low-molecular weight beta-glucan

A low molecular weight, glucan technology, applied in beer brewing, fermentation and other directions, can solve the problems of product turbidity, high viscosity, β-glucan purification, etc., and achieve the effect of reducing molecular weight, reducing diabetes, and improving purity

Inactive Publication Date: 2014-06-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the patent ZL201210460834.1 invented a kind of brewed product that mixes crude β-glucan with a content of about

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 A preparation method of low molecular weight β-glucan

[0019] Prepare a 2% aqueous solution of commercially available food-grade oat β-glucan with a β-glucan content of about 70% (Hangzhou Zhongzhi Kang Mushroom, 70% purity), and dissolve it in a water bath at 70-80°C. Then cool to 37-55°C, add endo-β-glucanase (Genencor, cellulase) to treat for 0.5-4h, monitor viscosity (2%, <100cp) and molecular weight (10KDa-100KDa), add activated carbon Decolorize for 1 hour, filter to remove activated carbon, collect dextran with a molecular weight of 10KDa-100KDa with an ultrafiltration membrane, and freeze-dry to obtain a low molecular weight β-glucan product with a purity of about 95%.

Embodiment 2

[0020] Example 2 A preparation method of low molecular weight β-glucan

[0021] Prepare a 2% aqueous solution of commercially available food-grade oat glucan with a β-glucan content of about 98% (Shanghai Wandao, 98% purity), dissolve it in a water bath at 70-80°C, and then cool it to 37 -55°C, add endo-β-glucanase (Genencor, cellulase) to treat for 0.5-4h, monitor viscosity (2%, <100cp) and molecular weight (10KDa-100KDa), and collect molecular weight with ultrafiltration membrane The 10KDa-100KDa glucan is lyophilized to obtain a low molecular weight β-glucan product with a purity of 98%.

Embodiment 3

[0022] Example 3 Application of low molecular weight β-glucan in beer

[0023] Prepare high-purity low-molecular-weight β-glucan into a 10% transparent solution, add it to beer (Tsingtao Brewery, wheat concentration 8°P, Shanghai Songjiang), and make the concentration of low-molecular-weight β-glucan in the beer reach 0.5g / L, 1 g / L, 2 g / L, 4 g / L, 6 g / L, after 15 days of storage at room temperature, it was found that the transparency of the beer remained unchanged, the chroma was in line with the national standard, the beer tasted thick and fragrant, and the beer's ability to hold foam Increase.

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Abstract

The invention discloses a preparation method and a use of low-molecular weight beta-glucan and belongs to the technical field of food and beverage processing. The preparation method comprises the following steps of preparing an aqueous solution having a mass concentration of 1-5% from marketed food-grade cereal beta-glucan, carrying out treatment by endo-type beta-glucanase for 0.5-4h, controlling viscosity (2%, <100cp) and molecular weight (of 10-100KDa), carrying out decoloring by active carbon, collecting beta-glucan having the molecular weight of 10-100KDa by an ultrafilter membrane, and carrying out freeze drying to obtain a product. The low-molecular weight beta-glucan can be used in foods such as beer and beverages, improves daily intake of beta-glucan, improves human immunity and reduces cholesterol.

Description

technical field [0001] The invention relates to a preparation method and application of low-molecular-weight β-glucan, belonging to the technical field of food and beverage processing. Background technique [0002] The characteristic of β-glucan is the main chain of glucose residues connected by β-1,3 glycosidic bonds, and the side chains of different types are different. For example, thermal gel is completely composed of β-1,3-glucose backbone, scleroglucan, yeast glucan, hydrangea polysaccharide and schizophyllan contain β-1,6-glucosyl side chains, while barley, barley The main chain of β-glucan contained in oats contains β-1,3 and β-1,4-glucosidic bonds in a ratio of about 1:2.4, but there are no branches. At present, β-glucan is used in various industries such as pharmaceuticals, health care products, beauty and skin care products, food additives, and plant vaccines. In medicine, it can assist in the treatment of tumors, lower blood pressure, and lower blood lipids; in...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12C5/00
Inventor 詹晓北吴剑荣朱莉郑志永张洪涛
Owner JIANGNAN UNIV
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