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Biological method for inducing heartwood formation of dalbergia odorifera

A technology of Dalbergia Dalbergia and Dalbergia Dalbergia, which is applied in the field of stimulating Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Dalbergia Aromatic

Inactive Publication Date: 2014-06-04
海南省林业科学研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the method of using the naturally formed heartwood endophytic bacteria of Dalbergia to induce Dalbergia to rapidly form heartwood

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Drill out 5g of heartwood with a growth cone from a 60-year-old Dalbergia japonica tree, disinfect the surface with 70% alcohol, grind and pulverize it under sterile conditions, put it into 200mL ISP2 liquid medium, and culture it with shaking at 28°C and 180rpm After 1 week, the culture solution of the endophyte strain of Aroma burdiflora was prepared. Choose 30 strains of Dalbergia japonica trees that have not formed heartwood in 6-7 years, use a drill bit with a diameter of 1 cm to drill 3 holes (inclination 45°) from top to bottom on the trunk in the range below the height of 50 cm from the ground, and the depth of the hole is up to For the xylem, the upper and lower intervals of each hole are 10-15cm. Inject 1mL of culture solution into each hole, seal and wrap it with plastic wrap and scotch tape. After 12 months of treatment, cut down the trees, and dig out the purple-red xylem from the treated part . The obtained purplish red substance was dried in the sun and ...

Embodiment 2

[0013] Drill out 3g of heartwood with a growth cone from a 40-year-old Dalbergia japonica tree, disinfect the surface with 70% alcohol, grind and pulverize it under sterile conditions, put it into 100mL ISP2 liquid medium, and culture it with shaking at 28°C and 180rpm After 1 week, the culture solution of the endophyte strain of Aroma burdiflora was prepared. Choose 30 strains of Dalbergia japonica trees that have not formed heartwood in 6-7 years, use a drill bit with a diameter of 1 cm to drill 3 holes (inclination 45°) from top to bottom on the trunk in the range below the height of 50 cm from the ground, and the depth of the hole is up to For the xylem, the upper and lower intervals of each hole are 10-15cm. Inject 1mL of culture solution into each hole, seal and wrap it with plastic wrap and scotch tape. After 12 months of treatment, cut down the trees, and dig out the purple-red xylem from the treated part . The obtained purplish red substance was dried in the sun and ...

Embodiment 3

[0015] Drill out 2g of heartwood with a growth cone from a 70-year-old Dalbergia japonica tree, and after surface disinfection with 70% alcohol, grind and pulverize it under sterile conditions, put it into 50mL ISP2 liquid medium, and culture it with shaking at 28°C and 180rpm After 1 week, the culture solution of the endophyte strain of Aroma burdiflora was prepared. Choose 30 strains of Dalbergia japonica trees that have not formed heartwood in 6-7 years, use a drill bit with a diameter of 1 cm to drill 3 holes (inclination 45°) from top to bottom on the trunk in the range below the height of 50 cm from the ground, and the depth of the hole is up to For the xylem, the upper and lower intervals of each hole are 10-15cm. Inject 1mL of culture solution into each hole, seal and wrap it with plastic wrap and scotch tape. After 12 months of treatment, cut down the trees, and dig out the purple-red xylem from the treated part . The obtained purplish red substance was dried in the ...

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Abstract

The invention belongs to the field of biotechnology and relates to a biological method for inducing heartwood formation of the dalbergia odorifera, heartwoods of adult dalbergia odorifera trees with formed heartwoods are collected in a drilling mode, smashed and then added into an ISP2 liquid medium for being cultivated, and an endophyte mycelium culture solution of rosewood heartwoods is prepared; the dalbergia odorifera trees of 6-7 years without the formed heartwoods are selected, drilling is performed on trunks, the depths of holes reach xylems, the endophyte mycelium culture solution is then injected into the holes, hole openings are sealed, and after 12 months, the claret-colored xylems dug from the processed parts are the rosewood heartwoods. The biological method is simple in technology and easy and convenient to operate, the endophyte mycelia of the rosewood heartwoods are used for inducing the rosewood heartwoods, the production period of heartwood formation of the dalbergia odorifera is effectively shortened, and the biological method has the advantages of being natural and free of hazard and is one of the safest and most promising methods. The biological method is suitable for large-scale standard and commercial production of the rosewood heartwoods, facilitates protection, development and utilization of dalbergia odorifera resources and has important economic, social and ecological benefits.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for stimulating the aroma formation of Dalbergia balata, in particular to a method for inducing the aroma formation of Dalbergia balata by adopting a biological method. Background technique [0002] The traditional Chinese medicine Jiangxiang is the dried heartwood of the trunk and root of the leguminous plant Dalbergia odorifera T.Chen. Jiangxiang mainly has the effects of promoting qi and relieving pain, promoting blood circulation, stopping bleeding, stopping dysentery, and stopping vomiting. It is a commonly used clinical preparation For example, the main raw materials of Chinese patent medicines such as Compound Jiangxiang Capsules, Guanxin Danshen Tablets, Rujie Xiaosan Tablets, etc., can be used to treat bruises, trauma, wrist pain, chest pain, etc., and have high medicinal value and economical value. value. In recent years, with the continuous increase of mark...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
Inventor 周双清黄小龙周亚东盛小彬林旭俊李晓斌
Owner 海南省林业科学研究所
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