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A Quick Method to Obtain Genetic Homogeneity of Wheat Powdery Mildew

A technology of wheat powdery mildew and genetic homogeneity, applied in the field of phytopathology, can solve the problems of prolonged purification of strains and material death

Inactive Publication Date: 2017-02-01
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the prolonged time for purifying strains, many materials died in the process of continuous purification

Method used

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  • A Quick Method to Obtain Genetic Homogeneity of Wheat Powdery Mildew

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Embodiment Construction

[0016] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is illustrated with the following specific examples, the present invention mainly carries out fast purification for wheat powdery mildew, but the present invention is not limited to these specific examples. The specific implementation method is as follows:

[0017] 1. Cultivation of healthy wheat seedlings

[0018] Select the susceptible wheat variety "Chuannong 26" or other susceptible varieties, soak the seeds in 75% ethanol for 3 minutes, rinse with sterile water 3 times, put them in a moisturizing container, and germinate them at 22°C for 1-2 days. Fill a large test tube with a diameter of 2.5 cm and a length of 20 cm into 3 cm thick sterile fine soil, then sow the germinated wheat seeds into the test tube, sow 5-7 grains in each test tube, cover with 5 layers of gauze or 1 layer filter membrane. (17±1)°C light incubator (12h light-dark alternation...

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Abstract

The invention discloses a fast method for obtaining genetically homogenized wheat powdery mildew. A single conidium is picked by a specially-made teasing needle and transferred onto an in-vitro leaf section under a stereo microscope. The leaf section is put in a culture dish containing benzimidazole (50ppm) preservative matrix, more than one spore chain is formed after light and dark alternative culturing for seven days, and powdery mildew piles with the diameter of about 0.5cm are formed after 17 days. At least three circulations are reduced in the one-time technological flow of the pure strain compared with the common purification technology, and 20-30 days can be saved. The strain obtained by adopting the method is entirely genetic homogeneous, and provides material guarantee for the follow-up study related to wheat powdery mildew.

Description

technical field [0001] The invention belongs to the technical field of plant pathology, and relates to a quick method for obtaining genetically homogeneous wheat powdery mildew (Blumeria graminis f.sp. tritici), which can also be used for purification of other powdery mildews besides the fungus. Background technique [0002] 1. Mainly used technical regulations at present [0003] The common purification technique is to use single spore piles to purify strains. The standard samples of wheat powdery mildew collected from various places were shaken off and inoculated on new wheat seedlings under isolation conditions, and the visible infection spots on the wheat leaves were checked 4 to 6 days after inoculation, and single infected spots with no sporulation were cut with scissors. The leaf segment of the stained spot is placed in a petri dish containing a benzimidazole fresh-keeping substrate, cultivated in a light incubator (8000lx light, 14h / d) at (17±1)°C, and after the con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 龚国淑张敏陈华保梁银萍祁小波雷雨杨继芝杨春平
Owner SICHUAN AGRI UNIV
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