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Milk cattle mastitis vaccine

A dairy cow mastitis and vaccine technology, applied in biochemical equipment and methods, bacterial antigen components, microorganisms, etc., can solve the problems of high attack rate of dairy cows, high actual loss, and spread of pathogenic microorganisms

Active Publication Date: 2014-05-21
INNER MONGOLIA HUAXI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The recessive type has no specific clinical manifestations and can only be detected through somatic cells, milk production and quality testing, but the attack rate of dairy cows is much higher than that of the clinical type, causing further spread of pathogenic microorganisms, and under certain conditions it will transform into clinical symptoms. type, the actual loss is much higher than the clinical type

Method used

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  • Milk cattle mastitis vaccine
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the acquisition of each bacterial strain

[0042] 1. Acquisition and preservation of E. coli recombinant bacteria

[0043] (1) Screen wild bacteria

[0044] 30,000 cow milk samples were collected from 90 medium and large dairy farms in 30 provinces and cities including Inner Mongolia, Chongqing, Guangzhou, Heilongjiang, Lanzhou, Hebei, and Shandong. Milk sample collection method of dairy cows: select cows with clinical symptoms of mastitis (or suspected subclinical mastitis), first scrub the udders with warm water, then scrub the udders with 0.2% bromogeramine, and finally wipe the nipples with 70% alcohol. At the same time, use 70% alcohol to wipe and disinfect the fingers. Squeeze out the first 2-3 handfuls of milk from each milk chamber to eliminate contaminated bacteria. Take at least 5mL of milk samples from each cow in a sterile milk sample cup.

[0045]Isolation and purification of Escherichia coli strains from milk samples. Colony morphological ...

Embodiment 2

[0088] Embodiment 2, the characteristic of bacterial strain

[0089] 1. Characteristics of strain EBMO9

[0090] Thaw the cryopreservation tube obtained in Step 1 (2) and 4 of Example 1 at room temperature, and perform the following steps on the P3 generation strain EBMO9:

[0091] (1) Immunogenicity of strain EBMO9

[0092] Rabbits were immunized with the P3 generation strain EBMO9 (single immunization, each by subcutaneous injection of 5×10 7 CFU / ml, 1 ml for each injection), 21 days later, blood was collected from the heart, and the serum was separated. The IgG antibody titer of the serum was detected by ELISA (serum dilution was obtained by gradient dilution with PBST buffer; the P3 generation strain EBMO9 was used as the coating source, and the coating concentration was 1×10 9 Bacteria / ml; goat anti-rabbit IgG enzyme-labeled secondary antibody; set negative control, that is, replace serum diluent with PBST buffer; detect the absorbance value at 450nm; the absorbance va...

Embodiment 3

[0141] Embodiment 3, the preparation of vaccine

[0142] 1. Preparation of capsular polysaccharide

[0143] (1) Thaw the cryopreservation tube obtained in step 2 (2) and 3 of Example 1 at room temperature, and carry out the following steps for the P3 generation strain:

[0144] 1. Take 3-5 typical colonies of strain SACP5-9, inoculate them in BHI medium, and cultivate them with shaking at 37°C and 150rpm for 18h.

[0145] 2. Take 2 parts by volume of the bacterial liquid obtained in step 1, inoculate 100 parts by volume of BHI medium, and ferment in a fermenter at 37°C (at the initial stage of fermentation, the bacterial concentration of strain SACP5-9 is 2×10 8 per ml, the temperature of the fermenter is 37°C, the initial rotation speed of the fermenter is 198r / min, the tank pressure is maintained at 0.05MPa, and the pH of the fermentation system is adjusted to 7.0-7.2 with 8% NaOH aqueous solution or 5% hydrochloric acid aqueous solution. Rotate the speed of the fermenter ...

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Abstract

The invention discloses a milk cattle mastitis vaccine. The milk cattle mastitis vaccine comprises inactivated strain EBMO9, inactivated strain SACP5-9, inactivated SACP8-6, inactivated SACP336-3, inactivated strain SAWR-6, inactivated strain SDTR-9 and inactivated strain SURF-5 as active ingredients. The vaccine disclosed by the invention has the advantages of being efficient, general, safe and stable, and diverse in dosage forms, and is a new milestone of development of milk cattle mastitis vaccines.

Description

technical field [0001] The invention relates to a cow mastitis vaccine. Background technique [0002] Bovine Mastitis is a major infectious disease that endangers the development of animal husbandry and food safety. Especially in recent years, with the change of the environment and the abuse of antibiotics, a variety of drug-resistant bacteria and even superbugs have emerged. The morbidity and mortality of dairy cow mastitis caused by infection are increasing year by year, seriously endangering the development of animal husbandry and food safety, and further endangering human health. The economic losses caused by cow mastitis amount to 3 billion U.S. dollars in the United States, 400 million U.S. dollars in the United Kingdom, and more than 3.5 billion U.S. dollars in my country. Cow mastitis is divided into clinical type and recessive type. The symptoms of the clinical type are: fever, swelling and pain in the udder area, decreased milk production, changes in whey propert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/018A61K39/09A61K39/085A61P31/04A61P15/14C12N1/20C12R1/19C12R1/445C12R1/46
Inventor 杨鹏辉王林叶孙治华王欢孙科孙艺萍段跃强李敏
Owner INNER MONGOLIA HUAXI BIOTECH
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