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Method for rapidly extracting fish collagen protein by using enzymolysis method

A technology of fish collagen and enzymatic hydrolysis, which is applied in the field of rapid extraction of fish collagen by enzymatic hydrolysis, can solve the problems of lower product yield, incomplete degreasing, pollution, etc., achieve uniform molecular weight distribution, shorten production time, and improve extraction efficiency.

Inactive Publication Date: 2014-05-14
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing fish collagen extraction methods often ignore the problem of fat removal in fish waste during the pre-treatment, making fat mixed into collagen affect the purity of collagen, and restrict its application in downstream fields
In addition, the method of fat removal generally adopts acid, alkali solution or organic solvent method. These methods are not only costly, but the degreasing is not complete, and the use of chemical reagents is easy to denature collagen and reduce product yield.
The traditional collagen extraction method uses acid or alkali to degrade collagen to extract, these methods will lead to the destruction of some amino acids during the degradation process, poor product safety, loss of biological activity, and waste liquid in production will pollute the environment; low-temperature biological enzymatic hydrolysis Technology, without adding other substances, can improve product quality (such as CN102533914 A) and avoid environmental pollution, but it takes a long time, the product molecular weight is low, and the obtained collagen molecular weight distribution is uneven

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for rapidly extracting fish collagen by enzymatic hydrolysis, comprising the steps of:

[0027] (1) Cut the fish skin, fish scales, and swim bladders that have been cleaned of surface impurities into small pieces, and cut them into 50-mesh fish meal with a crusher;

[0028] (2) Add fishmeal into a closed container, then add distilled water with 3 times the mass of fishmeal and reaction enzyme to make a slurry, wherein the reaction enzyme is composed of neutral lipase and bromelain, and the amount of reaction enzyme added accounts for 0.1% of the mass of fishmeal. %, and the mass ratio of neutral lipase and bromelain is 1: 4, the prepared slurry is controlled under 1.5 standard atmospheric pressure, 15kHz ultrasonic treatment, the reaction temperature is 50°C, the pH is 7, and the enzymolysis reaction is carried out after stirring for 2h;

[0029] (3) Add 0.1% of the mass of sodium hydroxide to the slurry obtained after the reaction in step (2), heat to 90°C, an...

Embodiment 2

[0032] A method for rapidly extracting fish collagen by enzymatic hydrolysis, comprising the steps of:

[0033] (1) Cut the fish skin, fish scales, and swim bladders that have been cleaned of surface impurities into small pieces, and cut them into 100-mesh fish meal with a crusher;

[0034] (2) Add fishmeal into a closed container, then add distilled water and reaction enzyme, which account for 5 times the mass of fishmeal, and stir to make a slurry. 0.3%, and the mass ratio of neutral lipase and alkaline protease is 1: 4, the prepared slurry is controlled under 2 standard atmospheric pressure, 20kHz ultrasonic treatment, the reaction temperature is 55 ℃, the pH is 7.5, and the enzyme is stirred for 1.5h solution reaction;

[0035] (3) Add 0.2% of the mass of sodium hydroxide to the slurry obtained after the reaction in step (2), heat to 80°C, and stir for 30 minutes;

[0036] (4) Filter the slurry, place the filtrate in a dialysis bag with a molecular weight cut-off of 2KD,...

Embodiment 3

[0038] A method for rapidly extracting fish collagen by enzymatic hydrolysis, comprising the steps of:

[0039] (1) Cut the fish skin, fish scales, and swim bladders that have been cleaned of surface impurities into small pieces, and cut them into 150-mesh fish meal with a crusher;

[0040](2) Add fishmeal into a closed container, then add distilled water and reaction enzyme which account for 7 times the mass of fishmeal and stir to make a slurry, wherein the reaction enzyme is composed of neutral lipase and trypsin, and the amount of reaction enzyme added accounts for 0.5% of the mass of fishmeal. %, and the mass ratio of neutral lipase to trypsin is 1:3, the prepared slurry is controlled under 2.5 standard atmospheric pressure and 25kHz ultrasonic treatment, the reaction temperature is 70 ° C, the pH is 7.5, and the enzymolysis reaction is carried out by stirring for 1 h;

[0041] (3) Add 0.3% of the mass of sodium hydroxide to the slurry obtained after the reaction in step ...

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Abstract

The invention discloses a method for rapidly extracting fish collagen protein by using an enzymolysis method. Degreasing treatment and protein extraction are accomplished in one step by using a high pressure ultrasonic enzymolysis agent, the extraction efficiency is greatly improved, and the protection time is shortened. The extraction process is environment-friendly, rapid and convenient, uniform in fish collagen protein molecular weight distribution, and small in impurity.

Description

technical field [0001] The invention belongs to the technical field of biological product preparation, in particular to a method for rapidly extracting fish collagen by enzymatic hydrolysis. Background technique [0002] Collagen is an important structural protein with rich physiological functions in connective tissue. Its unique macromolecular helical structure and aggregated structure make it have certain thermal stability and high mechanical toughness and strength. It is unique for food, pharmaceuticals, and packaging materials. Collagen has strong hydrophilic and moisturizing properties. It can be used as an additive in food to improve the flavor of food and increase the storage stability of food. It can be used as a gelling agent, stabilizer, emulsifier, enhancer Thickener, foaming agent, adhesive, clarifying agent, etc. Collagen, as a natural biological resource, is now more and more widely used in high value-added industries such as food, medicine, cosmetics, and nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 钟春燕钟宇光
Owner 钟春燕
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