Eu<3+> and rhodamine 6G doped polymer fluorescent encoding microsphere and preparation method

Abstract

The invention relates to a Eu<3+> and rhodamine 6G doped polymer fluorescent encoding microsphere and a preparation method. The preparation method comprises the steps of dispersing a surface sulfonic polystyrene-divinyl benzene cross-linked microsphere in a mixed solvent, adding a Eu<3+> solution to obtain a Eu<3+> doped polymer fluorescent encoding microsphere, evenly dispersing the Eu<3+> doped polymer fluorescent encoding microsphere in the deionized water solution of CH3COOH and (NH4)2S2O8, adding rhodamine 6G, and carrying out shaking, centrifugal washing and freeze drying to obtain the Eu<3+> and rhodamine 6G doped polymer fluorescent encoding microsphere. After the prepared fluorescent encoding microsphere is motivated, three fluorescent emissions can be obtained; and through changing the addition of europium or rhodamine 6G, a series of fluorescent encoding microspheres with different fluorescent emission strength ratios can be prepared and can be applied to the fields of biological detection, medical diagnosis and the like.

Description

technical field [0001] The present invention relates to a polymer fluorescent coded microsphere and its preparation method, in particular to doping with different amounts of europium ions or rhodamine 6G to obtain a series of polystyrene with different europium ions, rhodamine 6G and surface sulfonate - The invention relates to a fluorescent coded microsphere with a fluorescence emission intensity ratio of a divinylbenzene cross-linked (PSDVB) microsphere, which belongs to the technical field of luminescent materials. Background technique [0002] Europium has a unique luminescent property. After being excited, it can emit a rich fluorescence spectrum, and can emit electromagnetic radiation of various wavelengths from ultraviolet to infrared. The typical red fluorescence around 611nm has been developed as a variety of important luminescent materials. . Rhodamine 6G is a kind of rhodamine fluorescent dye, which has the advantages of good photostability, insensitivity to pH, ...

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Problems solved by technology

literature( Colloids and Surfaces A: Physicochem. Eng. Aspects 326 (2008) 109–114) reported doping europium ions onto inorganic silicon spheres to obtain europium-doped inorganic fluorescent silicon spheres. This method simply doped a fluorescent substance onto inorganic silicon spheres to obtain A fluorescent microsphere emitting fluorescence, and the particle size of the silicon sphere obtained is not uniform

literature( J. Mater. Chem. , 2010, 20, 1179–1185) reported that rhodamine 6G was doped onto polystyrene microspheres to obtain rhodamine 6G doped fluorescent microspheres, and a series of different rhodamine 6G Fluorescence-coded microspheres with fluorescence emission intensity, the experimental conditions of this method are cumbersome, and only one fluorescent dye is doped on the microspheres, and only one kind of fluorescent emission coded microspheres can be obtained

[0004] Before the present invention was made, Suzhou Nanomicrobe Technology Co., Ltd. produced and sold a surface sulfonated polystyrene-divinylbenzene cross-linked (PSDVB) microsphere with a maximum excitation wavelength of about 330 nm (300 ~ 350nm), the maximum emission wavelength is around 395nm, the microsphere has only one emission wavelength after being excited, and cannot be used for fluorescent coding, so it can only be used as a simple matrix ball, and its application range is limited

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