Preparation method for elaeis guineensis leaf scanning electron microscope sample
A scanning electron microscope and sample preparation technology, applied in the preparation of test samples, etc., can solve the problems of unfavorable identification and classification of oil palm germplasm resources, and the inability to identify and provide germplasm resources, so as to achieve clear leaf surface structure Integrity, complete sample structure, low cost effect
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[0022] 1. Preparation of fixative: FAA fixative was obtained by volume ratio of 90ml of 70% alcohol + 5ml of glacial acetic acid + 5ml of formaldehyde.
[0023] 2. Material pretreatment: Take the third leaf from the top of the 2-year-old oil palm seedling, wash and dry it, and quickly cut it with a blade to cut the middle part of the leaf and small sections of about 1cm×1cm on both sides of the midrib, and put them into a 2ml centrifuge tube , first rinsed with pure water for 3 times, then fixed with FAA fixative, stored at 4°C for 24 hours, taken out and rinsed with pure water, and then used for the next step of dehydration. (Because it is not easy to distinguish the upper and lower epidermis of the dried leaves, in order to unify the observation, a right-angled trapezoidal method is adopted to distinguish, and the upper epidermis of the leaf is cut into a right-angled trapezoid, and the step of trimming the blade before coating , let the acute angle of the right-angled trape...
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