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Biochip for enriching and detecting circulating tumor cells and preparation method thereof

A technology of tumor cells and biochips, which is applied in the field of biomedical materials and functional materials, can solve the problems of complex manufacturing process, low enrichment efficiency, long enrichment and detection time, and achieve high transparency, efficient enrichment, The effect of high-efficiency and high-sensitivity enrichment

Inactive Publication Date: 2015-07-08
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned technical solutions have certain functions of enriching circulating tumor cells, they also have different deficiencies, such as the complicated manufacturing process, some enrichment efficiency is not high, and some enrichment and detection time are long, etc.

Method used

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  • Biochip for enriching and detecting circulating tumor cells and preparation method thereof
  • Biochip for enriching and detecting circulating tumor cells and preparation method thereof
  • Biochip for enriching and detecting circulating tumor cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Ultrasonic cleaning of 1×1 square centimeter quartz slices in acetone, absolute ethanol, and deionized water for 10 minutes, and then using Piranha solution (concentrated H 2 SO 4 / 30%H 2 o 2 , V / V=7:3) for 30 minutes, then ultrasonic cleaning in deionized water for 10 minutes until the quartz piece is clean, and finally fully blown dry with nitrogen; light a candle, wait for the flame to stabilize (about 5 minutes), use tweezers Place the quartz sheet on the burning flame of the candle, and deposit a soot layer with a thickness of 20 microns on the surface of the quartz sheet and composed of nano-soot particles;

[0040] (2) Place the quartz sheet obtained in step (1) and deposited with a soot layer composed of nano-soot particles in a petri dish (D=40mm, H=11mm), add 0.1mL silicon tetrachloride liquid, seal and place In the fume hood, the quartz sheet was taken out after 1 hour, and a silicon dioxide shell layer with a thickness of 20 nanometers was chemically ...

Embodiment 2

[0051] (1) Ultrasonic cleaning of 1×1 square centimeter quartz slices in acetone, absolute ethanol, and deionized water for 10 minutes, and then using Piranha solution (concentrated H 2 SO 4 / 30%H 2 o 2 , V / V=7:3) for 30 minutes, then ultrasonic cleaning in deionized water for 10 minutes until the quartz piece is clean, and finally fully blown dry with nitrogen; light a candle, wait for the flame to stabilize (about 5 minutes), use tweezers Place the quartz sheet on the burning flame of the candle, and deposit a layer of soot layer composed of nano-soot particles with a thickness of 500 microns on the surface of the quartz sheet;

[0052] (2) Place the quartz sheet obtained in step (1) and deposited with a soot layer composed of nano-soot particles in a petri dish (D=40mm, H=11mm), add 0.1mL silicon tetrachloride liquid, seal and place In the fume hood, the quartz sheet was taken out after 72 hours, and a silicon dioxide shell layer with a thickness of 200 nanometers was ch...

Embodiment 3

[0063] (1) Ultrasonic cleaning of a 1×1 square centimeter quartz plate in acetone, absolute ethanol, and deionized water for 10 minutes, and then using Piranha solution (concentrated H 2 SO 4 / 30%H 2 o 2 , V / V=7:3) for 30 minutes, then ultrasonic cleaning in deionized water for 10 minutes until the mica sheet is clean, and finally fully blown dry with nitrogen; light the burning lamp of vegetable oil, and wait for the flame to stabilize (about 5 minutes ), use tweezers to pick up the mica sheet and place it on the flame of burning vegetable oil, and deposit a soot layer with a thickness of 1 micron on the surface of the mica sheet and composed of nano-soot particles;

[0064] (2) Place the mica sheet obtained in step (1) and deposited with a soot layer composed of nano-soot particles in a petri dish (D=40mm, H=11mm), add 0.1mL silicon tetrachloride liquid, seal it and place In the fume hood, the mica sheet was taken out after 10 minutes, and a silicon dioxide shell layer wi...

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Abstract

The invention relates to a biochip for enriching and detecting circulating tumor cells and preparation method thereof. The invention comprises the following steps: placing a clean substrate on flame, obtaining a cigarette ash layer composed of nano cigarette ash particles on surface of the substrate by deposition, using the nano cigarette ash particle as template, and deposing a layer of silica housing layer on external surface of nano cigarette ash particles by chemical vapor deposition method; removing nano cigarette ash particles in the silica housing layer by high temperature calcining, and obtaining a silicon dioxide layer composed of nano silica particles with micrometer thickness; finally, modifying an anti-EpCam antibody which can specifically identify tumor cells on the surface of the silicon dioxide layer, thereby obtaining a biochip for enriching and detecting circulating tumor cells. The biochip can enrich circulating tumor cells with high efficiency and high sensitivity, and the product has high transparency under water and can be used for detecting real time captured circulating tumor cells.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and functional materials, and in particular relates to a biochip for enrichment and detection of circulating tumor cells and a preparation method thereof. Background technique [0002] As early as 1896, Australian pathologist Thomas Ashworth (Thomas Ashworth) reported that circulating tumor cells (circulating tumor cells, CTCs) were observed in the blood of a patient with metastatic cancer — detached from solid tumors and Tumor cells entering the bloodstream. Now, it is generally believed that the detection of circulating tumor cells can be effectively used in early diagnosis in vitro, rapid assessment of chemotherapy drugs, individualized treatment including clinical drug screening, detection of drug resistance, monitoring of tumor recurrence and development of new tumor drugs, etc. . Nowadays, with the in-depth research on the mechanism of tumor metastasis, especially with the wid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCC12M33/10
Inventor 王树涛杨高江雷
Owner INST OF CHEM CHINESE ACAD OF SCI
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