Natural pregnant mare conjugated estrogen purification and refinement method and application of nonionic nonpolar fine separation resin used by same

Abstract

The invention relates to a natural pregnant mare conjugated estrogen purification and refinement method and application of a nonionic nonpolar fine separation resin used by the same. The method comprises the following steps: 1. adsorbing an initial liquid containing a pregnant mare conjugated estrogen crude extract by using a nonionic nonpolar fine separation resin; and 2. eluting the nonionic nonpolar fine separation resin carrying the conjugated estrogen mixture by using an eluting solvent to obtain the purified and refined conjugated estrogen mixture liquid. Compared with the prior art, the purity of the conjugated estrogen prepared by the method is up to 42.6-61.8%, and the total conjugated estrogen yield is 90.8-97.2%; and the conjugated estrogen has no peculiar smell and obviously lighter color (fine light yellow), which is very important for preparing the high-quality conjugated estrogen active pharmaceutical ingredient and preparation products. The method has the advantages of simple technique and high refinement efficiency, and is especially suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of purifying and refining the crude extract of natural pregnant horse-conjugated estrogen by using a fine separation resin, and relates to a purification method for natural pregnant horse-conjugated estrogen and the application of the non-ionic and non-polar fine separation resin used therein. Background technique [0002] Conjugated estrogen drugs are mainly used in hormone replacement therapy (Hormone Replacement Therapy, HRT), to relieve any clinical symptoms of estrogen deficiency, to treat and prevent menopausal syndrome after women's physiological or artificial menopause, and to prevent and treat osteoporosis. The latest clinical research on osteoporosis, coronary heart disease and senile dementia found that early HRT can prevent coronary heart disease and senile dementia. Natural conjugated estrogens have been used for a long time for their good efficacy and tolerability. [0003] Conjugated estrogen...

AI Technical Summary

Problems solved by technology

[0014] The present invention provides a method for purifying and refining natural pregnant horse-conjugated estrogen and the application of the non-ionic and non-polar fine separation resin used; The polar fine separation resin is used for the purification and refining process engineering of extracting natural conjugated estrogen from pregnant horse urine; it overcomes the shortcomings of the existing technology and avoids the use of a large amount of toxic organic solvents for liquid-liquid extraction, environmental pollution and toxic solvent residues In addition to the problem of medicine, it also solves the problems of low refining efficiency, low purity and low yield of the obtained conjugated estrogen; the present invention makes the obtained conjugated estrogen high in purity, high in total conjugated estrogen yield, and has no peculiar smell , The color is obviously lightened, and it is delicate and light yellow. The process of the present invention is simple, the refining efficiency is high, and it is especially suitable for large-scale production

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