Pseudomonas aeruginosa and application of pseudomonas aeruginosa in aspect of degrading aflatoxin

A technology of Pseudomonas aeruginosa and aflatoxin, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of complex degradation products, unstable effects, and large loss of nutrients, and achieve good results The effect of applying the foreground

Active Publication Date: 2014-04-09
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Traditional aflatoxin detoxification methods include physical and chemical methods, including ammoniation method, alkali method, high temperature method, ray irradiation method and ultrafiltration-diafiltration method, etc. These methods have unstable effects, large loss of nutrients, The degradation products are complicated, the toxicity of the degradation products is difficult to determine, and it is difficult to produce on a large scale; in addition, the adsorption method also adsorbs nutrients while adsorbing toxins

Method used

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  • Pseudomonas aeruginosa and application of pseudomonas aeruginosa in aspect of degrading aflatoxin
  • Pseudomonas aeruginosa and application of pseudomonas aeruginosa in aspect of degrading aflatoxin
  • Pseudomonas aeruginosa and application of pseudomonas aeruginosa in aspect of degrading aflatoxin

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Experimental program
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Embodiment 1

[0031] Embodiment 1, the isolation and identification of bacteria

[0032] 1. Isolation of bacteria

[0033] (1) In June 2013, the collected soil samples were shaken in sterile distilled water for 15 minutes in an ultra-clean workbench to prepare a bacterial suspension, and the shaker speed was 180rpm.

[0034] (2) Gradiently dilute the bacterial suspension with sterile distilled water and spread it on the NA medium plate, culture it at 30°C for 24 hours, the colonies cover the whole plate, pick the shape, size and color of the plate with an inoculation loop Strains with different transparency were purified by streaking on the plate, and the purified strains were applied to the aflatoxin degradation experiment. The obtained strain with the strongest aflatoxin degradation ability was named N17-1.

[0035] 2. Identification

[0036] (1) According to the method described in "Berger's Bacteria Identification Handbook" (Eighth Edition) and "Common Bacteria System Identification H...

Embodiment 2

[0048] Embodiment 2, the cultivation of Pseudomonas aeruginosa N17-1

[0049] Pseudomonas aeruginosa (Pseudomonas aeruginosa) N17-1 (CGMCC No.8511) was inoculated in liquid NB medium, shaken and cultured for 24 hours at a temperature of 37 °C and a rotation speed of 200 rpm (rotation radius 20 mm) to obtain bacteria solution for aflatoxin degradation experiments.

Embodiment 3

[0050] Embodiment 3, Pseudomonas aeruginosa N17-1 to aflatoxin B 1 Degradation

[0051] 1. Aflatoxin B 1 preparation

[0052] 1 mg aflatoxin B 1 (AFB 1 ) Standards were dissolved in 2ml of chromatographically pure methanol to obtain aflatoxin B with a concentration of 500ppb 1 the solution

[0053] Two, get the bacterium liquid that 0.4ml embodiment 2 obtains and place in the 1.5ml centrifuge tube, add the aflatoxin B that 0.1ml concentration is 500ppb 1 To a final concentration of 100 ppb, mix well and let stand at 37°C for 72 hours, then centrifuge at 10,000g for 10 minutes to remove cells, and obtain supernatant, which is recorded as the solution of the experimental group. Add 0.1ml of aflatoxin B with a concentration of 500ppb to 0.4ml of NB medium without bacteria 1 As a control group, it was recorded as a control group solution.

[0054] 3. N17-1 to aflatoxin B 1 Degradation effect detection

[0055] First, 100% methanol was added to the experimental group solu...

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Abstract

The invention discloses pseudomonas aeruginosa and application of the pseudomonas aeruginosa in the aspect of degrading aflatoxin. Preservation No. of the pseudomonas aeruginosa disclosed by the invention is CGMCC No. 8511. The pseudomonas aeruginosa disclosed by the invention can degrade aflatoxin effectively; the bacterium, as a biomaterial for degrading the aflatoxin, has excellent application prospects in development of new biodegradable bacterium or biodegradable sterile preparation.

Description

technical field [0001] The invention relates to a strain of Pseudomonas aeruginosa and its application in degrading aflatoxin. Background technique [0002] Aflatoxin (AFT) is a class of toxic secondary metabolites mainly produced by Aspergillus flavus and A. parasiticus fungi, with carcinogenic, teratogenic and mutagenic effects, only 0.294 mg / kg dose can cause acute poisoning death in sensitive animals (Bondy and Pestka, 2000; Wang et al., 2002; Rawal, et al., 2010), and its main target is the liver, which is the primary cause of malignant tumors. It is one of the main factors of hepatocellular carcinoma (HCC), and can also cause acute lesions of the kidney and adrenal gland (Poirier et al., 2000; Kensler et al., 2011). [0003] The basic structure of aflatoxin is difuran ring and oxinone (coumarin), the former is the basic toxic structure, and the latter has enhanced toxicity and carcinogenic effect. At present, there are about 20 kinds of aflatoxins that have been foun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/385A62D101/28
Inventor 赵月菊刘阳兰茨纳·桑格邢福国周露王龑
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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