Method for detecting drug-resistant mutation sites of campylobacter jejuni carbostyril antibiotics

Abstract

The invention discloses a method for detecting drug-resistant mutation sites of campylobacter jejuni carbostyril antibiotics. The method comprises the following steps: by taking campylobacter jejuni of which the 257th site of a gyrA gene coding sequence is C as a wild type standard strain, taking campylobacter jejuni of which the 257th site of the gyrA gene coding sequence is T as a mutant type standard strain, respectively and simultaneously performing high-resolution melting curve analysis with campylobacter jejuni to be detected; determining whether the 257th site of the gyrA gene coding sequence of the campylobacter jejuni to be detected is C or T according to the comparison result of the high-resolution melting curve; if the high-resolution melting curve of the campylobacter jejuni to be detected is consistent with that of the wild type standard strain, selecting the campylobacter jejuni to be detected as a strain of which the 257th site of the gyrA gene coding sequence is C, and if the high-resolution melting curve of the campylobacter jejuni to be detected is consistent with that of the mutant type standard strain, selecting the campylobacter jejuni to be detected as a strain of which the 257th site of the gyrA gene coding sequence is T.

Description

technical field [0001] The invention relates to a method for detecting quinolone antibiotic resistance mutation sites of Campylobacter jejuni. Background technique [0002] Campylobacter jejuni (Campylobacter jejuni), also known as Campylobacter jejuni, is one of the most important food-borne pathogens that cause acute gastroenteritis in humans. Caused by Campylobacter coli. Campylobacter jejuni can be transmitted to humans through the food chain, and contaminated poultry meat, milk and drinking water are the main routes of transmission. Quinolones are the most commonly used drugs in the treatment of enteritis caused by Campylobacter jejuni, and they are also the first-line treatment drugs for Campylobacter infection in clinical practice. The emergence of drug-resistant Campylobacter jejuni leads to clinically protracted course of Campylobacter jejuni disease and treatment failure, which poses a great threat to human health. At the same time, drug-resistant strains also le...

AI Technical Summary

Problems solved by technology

Traditional genotype mutation detection methods mainly include polymerase chain reaction-linear probe method, polymerase chain reaction-restriction fragment length polymorphism analysis method, mismatch amplification mutation polymerase chain reaction method and fluorescence quantitative polymerization Enzyme chain reaction and other methods, the above-mentioned genotype mutation detection methods based on polymerase chain reaction are relatively expensive, and are not suitable for detecting a large number of samples, and they are only qualitative tests, which cannot be used for point mutations. qualitative test

The traditional drug susceptibility test requires the culture of Campylobacter jejuni, which requires high culture conditions and takes a lot of time, which does not meet the needs of emergency prevention and control of acute infectious diseases

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