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Method for synthesizing glutathione through fermentation

A technology of glutathione and fermentation liquid, which is applied in the directions of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problem of low glutathione concentration, etc. The effect of inhibitory concentration

Inactive Publication Date: 2014-04-02
CHENGDU YATU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When glutathione is synthesized by fermentation, due to the feedback inhibition in the process of microbial metabolism, when the glutathione produced by fermentation reaches a certain concentration, it will inhibit the further synthesis of glutathione, making the glutathione produced by fermentation The concentration of glycine is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for fermenting and synthesizing glutathione, comprising the following:

[0020] a. culturing yeast seed liquid; the yeast is one of Saccharomyces cerevisiae, baker's yeast, Candida and Pichia pastoris;

[0021] b. inoculation: inoculate the seed solution obtained in step a into the fermentation medium rich in sulfate, and the inoculum size is 10%;

[0022] c. Fermentation culture: the inoculated medium obtained in step b is cultivated and fermented, and the fermentation temperature is 25-30°C;

[0023] d. Supplementing cysteine: after 20 hours of fermentation, add cysteine ​​to the fermentation broth to keep the concentration of cysteine ​​in the fermentation broth at 0.1-2mmol / L;

[0024] e. End of fermentation and separation: After 48-120 hours of fermentation, the fermentation is ended, and the obtained fermentation broth is extracted and separated to obtain the glutathione.

Embodiment 2

[0026] A method for fermenting and synthesizing glutathione, comprising the following:

[0027] a. Cultivate the yeast seed liquid; the yeast is one of Saccharomyces cerevisiae, baker's yeast, Candida and Pichia pastoris; the concentration of the yeast seed liquid is the OD measured by ultraviolet light at a wavelength of 600nm The value is 1.0~1.5;

[0028] b. Inoculation: Inoculate the seed liquid obtained in step a into a fermentation medium rich in sulfate (pH of the fermentation medium=5.9~6.0), the inoculation amount is 10%;

[0029] c. Fermentation culture: the inoculated medium obtained in step b is cultivated and fermented, and the fermentation temperature is 25-30°C;

[0030] d. Supplementing cysteine: after 20 hours of fermentation, add cysteine ​​to the fermentation broth to keep the concentration of cysteine ​​in the fermentation broth at 0.1-2mmol / L;

[0031] e. End of fermentation and separation: After 48-120 hours of fermentation, the fermentation is ende...

Embodiment 3

[0033] A method for fermenting and synthesizing glutathione, comprising the following:

[0034] a. Cultivate yeast seed liquid: first recover the yeast strain in solid medium, pick a single colony, add it to the medium for cultivation, until its concentration is 1.0~ 1.5 o'clock, promptly get described yeast seed liquid; Described yeast is a kind of in Saccharomyces cerevisiae, baker's yeast, Candida and Pichia pastoris;

[0035] b. Inoculation: Inoculate the seed liquid obtained in step a into a fermentation medium rich in sulfate (pH of the fermentation medium=5.9~6.0), the inoculation amount is 10%;

[0036] c. Fermentation culture: the inoculated medium obtained in step b is cultivated and fermented, and the fermentation temperature is 25-30°C;

[0037] d. Supplementing cysteine: after 20 hours of fermentation, add cysteine ​​to the fermentation broth to keep the concentration of cysteine ​​in the fermentation broth at 0.1-2mmol / L;

[0038] e. End of fermentation and...

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PUM

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Abstract

The invention provides a method for synthesizing glutathione through fermentation, belonging to the field of production of glutathione. According to the method for synthesizing glutathione through fermentation, parameters such as concentration of a seed bacteria liquid, inoculum size and pH of a fermentation medium are optimized, the growth activity and fermentation activity of the yeast are maintained, the output of the glutathione is improved; and in fermentation, cysteine is added into the fermentation medium, the adding time and adding volume are optimized, so that the inhibition concentration of the glutathione can be improved, and the yield of the synthesized glutathione is further improved.

Description

technical field [0001] The invention belongs to the field of glutathione production, in particular to a method for fermenting and synthesizing glutathione. Background technique [0002] Glutathione (GSH) is a biologically active tripeptide compound containing γ-glutamyl and sulfhydryl groups condensed from glutamic acid, cysteine, and glycine. It is used in the synthesis of proteins and DNA, Amino acid transport, cell protection and other important biological phenomena play a direct or indirect role. It is mainly distributed in animals, plants, and microbial cells, and is widely used in many fields such as clinical medicine, sports health care, and food processing. Therefore, GSH has become a hotspot of research and exploration by scientists from all over the world. [0003] At present, the production methods of glutathione at home and abroad mainly include extraction method, chemical synthesis method, fermentation method and enzymatic method. Since the production of GS...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/865C12R1/72C12R1/84
Inventor 朱辉黄运昌
Owner CHENGDU YATU BIOLOGICAL TECH
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