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Bacillus having alga-lysing activity and application thereof

A bacillus and algae-dissolving technology, applied in the field of environmental microorganisms, can solve problems such as limited processing capacity and secondary pollution, and achieve the effects of short preparation period, good lethal effect and simple preparation method

Inactive Publication Date: 2014-04-02
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the limited processing capacity of physical and chemical methods in the prior art, and the defects of easily causing secondary pollution, the technical problem to be solved in the present invention is to find efficient algae-dissolving bacteria and to separate and enrich the highly efficient algae-dissolving active substances produced by the metabolism of algae-dissolving bacteria , for safe and efficient control of cyanobacterial blooms

Method used

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  • Bacillus having alga-lysing activity and application thereof
  • Bacillus having alga-lysing activity and application thereof
  • Bacillus having alga-lysing activity and application thereof

Examples

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Effect test

Embodiment 1

[0035] The screening of embodiment 1 algae-lytic bacteria

[0036] Add 10mL of natural water samples collected from the Meiliang Bay waters of Taihu Lake to 90mL of algae liquid of Microcystis aeruginosa 9110 in the logarithmic phase, take the yellow algae liquid two days later, apply the gradient dilution method to the beef extract peptone medium agar plate, and cultivate at 28°C After 24 hours, take a plate with a moderate colony density, and select different strains according to the different colony shapes.

[0037] The screened strains were inoculated into 10 mL of beef extract peptone medium, cultured at 28°C and 200 rpm for 24 hours, and 10 mL of cultured bacterial liquid was added to 90 mL of algae liquid of Microcystis aeruginosa in logarithmic phase. In addition, 10 mL of sterilized beef extract peptone medium was also added to 90 mL of algae liquid as a control. All the algae fluids of the experimental group and the control group were cultured in the light incubator...

Embodiment 2

[0041] Identification of embodiment 2 bacillus Lzh-5 bacterial strain

[0042] Lzh-5, which has the strongest algicidal effect, was identified by morphological observation, staining, physiological and biochemical reactions, and 16srRNA gene sequence analysis. ×1.2~10μm, often arranged in pairs or chains, with round or square ends. Cell staining was mostly Gram-positive in young cultures and moved with perinatal flagella. The spores are oval, oval, and round, and can resist many adverse environments. Physiological properties with a wide variety of heat, pH and salt. Chemoheterotrophs with fermentative or respiratory metabolism. After 16srRNA gene sequence analysis and homology comparison, it was found that it had 99% homology with a certain Bacillus strain in GenBank, so it was identified as a Bacillus bacterium and named Bacillus Lzh-5. The strain has been preserved in the General Microbiology Center of the National Microbiological Culture Collection Management Committee, ...

Embodiment 3

[0043] The preparation method of embodiment 3 bacillus Lzh-5 fermented liquid and ethyl acetate extract thereof

[0044] Bacillus Lzh-5 was inoculated in sterilized beef extract peptone medium according to the inoculum amount of 1%, and cultured on a shaker at 200 rpm at 28°C for 48 hours to obtain a fermentation broth containing Bacillus Lzh-5. Add ethyl acetate into the fermentation broth at a ratio of 1:1, put it into a shaker and vibrate for 24 hours, and separate the upper layer solution, that is, the ethyl acetate extract. The ethyl acetate was evaporated to dryness, dissolved in water, and filtered through a microporous membrane with a pore size of 0.22 μm for further purification of metabolites.

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Abstract

The invention discloses bacillus having an alga-lysing activity and application of the bacillus in controlling cyanobacterial bloom. The bacillus sp. Lzh-5 which has an obvious alga-lysing activity is separated from Lake Taihu water body, and has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 8282; active alga-lysing ingredients, namely hexahydropyrrolo [1,2-alpha] pyrazine-1,4-dione and 3-isopropyl-hexahydropyrrolo [1,2-alpha] pyrazine-1,4-dione, are separated, purified and identified from a metabolic product of the bacillus, wherein median lethal doses LD50 of hexahydropyrrolo [1,2-alpha] pyrazine-1,4-dione and 3-isopropyl-hexahydropyrrolo [1,2-alpha] pyrazine-1,4-dione on microcystis aeruginosa 9110 are respectively 5.7mu g / mL and 19.4mu g / mL. The bacillus is applicable to research and production of novel biological algicides, and finally used for controlling cyanobacterial blooms of lakes.

Description

technical field [0001] The invention relates to the field of environmental microbes, in particular to a strain of Bacillus sp. Lzh-5 secreting algae-dissolving substances and its application in the control of cyanobacteria blooms. Background technique [0002] The current control technologies of cyanobacterial blooms can be classified into physical methods, chemical methods and biological methods. Physical methods such as mechanical algae removal, flocculation hair removal and electromagnetic field algae removal can be used as auxiliary control measures for algae blooms, but the disadvantages are high cost, complicated equipment, temporary treatment rather than root cause, and limited processing capacity; chemical methods such as herbicides Such chemical algicides can directly kill algae, but these chemicals have poor specificity, are highly toxic to aquatic organisms, and are likely to cause harmful substance residues and secondary pollution. [0003] Based on the defects ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/50C12R1/07
Inventor 杨虹李正华谭晶柳向龙
Owner SHANGHAI JIAO TONG UNIV
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