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Preparation method of active microbial agent of forage anti-diarrhea yeast

A live bacteria preparation, anti-diarrhea technology, applied in the field of agricultural biology

Inactive Publication Date: 2014-03-26
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to study the high-density fermentation process, protective agent parameters and freeze-drying process of Saccharomyces boulardii, provide a preparation method of live anti-diarrhea yeast preparation for feeding, and solve the problem of diarrhea in livestock and poultry, especially in young animals

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  • Preparation method of active microbial agent of forage anti-diarrhea yeast

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Embodiment Construction

[0020] The specific embodiment of the preparation method of a kind of feeding anti-diarrhea live yeast preparation provided by the invention is as follows:

[0021] The strain used in this example is Saccharomyces boulardii, which is deposited in the American Type Culture Collection (ATCC MYA-796).

[0022] a. Wash and cut 200g of peeled potatoes, add 800ml of water, boil for 30min, filter with four layers of gauze, add 20g of glucose and 20g of agar, stir and mix well, dilute to 1000ml, divide into test tubes, steam sterilize at 121℃ for 20 minutes Afterwards, take out the test tube and put the slant surface to make the potato dextrose agar slant medium;

[0023] b. The original starting strain Saccharomyces boulardii was streaked on the potato dextrose agar slant, 30°C, and cultured for 48 hours to activate;

[0024] c. 10g yeast extract powder, 20g peptone in 900ml water, steam sterilized at 121°C for 20min, add 100ml 20% glucose solution which was steam sterilized at 115°...

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Abstract

The invention relates to a preparation method of a high-density fermented and freeze-dried active microbial agent of a forage anti-diarrhea yeast, and belongs to the field of an agriculture biotechnology. The method comprises the following steps: inoculating an original starting strain (Saccharomyces boulardii, ATCC MYA-796) into yeast leaching peptone powder glucose liquid culture medium after activating; cultivating at 30 DEG C for 12-16 hours at 200-250rpm as a seed solution; inoculating the seed solution by 10% of inoculation amount, cultivating at 30 DEG C for 12 hours at 800rpm in liquid-expanded yeast leaching peptone powder glucose liquid culture medium, and then feeding 25% glucose for 6 hours, wherein the feeding amount is 20ml / h / L of fermentation liquor; centrifugally collecting thalli and adding protective agents (8% of glycerinum, 7.5% of skim milk powder, 7.5% of mycose and 0.9% of sodium chloride), wherein the mixing ratio of the protective agents to the thalli is 2:1; stewing and balancing for an hour after evenly mixing; pre-cooling at -80 DEG C for 12 hours, and then freeze-drying the thalli in a vacuum freezer dryer; weighing freeze-dried powder to count viable bacteria, wherein the viable bacteria count achieves 1.4*10<10> CFU / g.

Description

(1) Technical field [0001] The invention relates to a preparation method for high-density fermentation of anti-diarrhea yeast for feeding and a live bacterial preparation for protection and freeze-drying, and belongs to the field of agricultural biotechnology. (2) Background technology [0002] Saccharomyces boulardii, a subspecies of Saccharomyces cerevisiae, is a thermotropic, non-toxic yeast isolated from litchi in Indonesia. It differs from Saccharomyces cerevisiae in terms of metabolism, physiology and genetic characteristics. Conventional microecological preparations are easily affected by antibiotics, gastric acid, and bile, and their application is limited. The biological characteristics of yeast are completely different from those of bacteria. Saccharomyces boulardii is a physiological fungal preparation that will not be destroyed by gastric acid, bile acid, antibiotics, etc. , so its application is relatively wide. At the same time, yeast has good tolerance to pH...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16
Inventor 滕达王建华王秀敏管庆丰
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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