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Achromobacter marplatensis and application thereof

A technology of endophytic bacteria and sugar beet, applied in the direction of application, bacteria, biocide, etc., can solve the problems that the growth-promoting effect of endophytic bacteria in sugar beet has not been reported, and achieve good technical effects, increase sugar content, and promote growth

Active Publication Date: 2015-02-18
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on plant endophytes has become a research hotspot at home and abroad, but there is no report on the growth-promoting effect of sugar beet endophytes

Method used

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  • Achromobacter marplatensis and application thereof
  • Achromobacter marplatensis and application thereof
  • Achromobacter marplatensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: sugar beet endophytic bacteria Achromobacter marplatensis Isolation, screening and identification of TNSC2011 CGMCC No. 8192

[0033] (1) Isolation and screening of strains

[0034] Several strains of IAA-producing bacteria were isolated from healthy beet by gradient dilution method, and a strain numbered TNSC2011 was screened out, which can effectively promote crop growth and convert insoluble phosphate into bioavailable soluble phosphoric acid Salt, improves photosynthesis. It has outstanding growth-promoting effect through application in sugar beet cultivation.

[0035] The colonies of this strain are small and smooth, transparent, white, with moist surface, neat edges, and fast growth; through Gram staining and microscopic observation, it is found that the strain is Gram-negative bacteria. Microbiologically identified as Achromobacter marplatensis Bacteria, rod-shaped cells, 0.8-1.0um×1.5-2um, Gram-negative (-), no spores, medium and smooth colo...

Embodiment 2

[0047] Embodiment two: sugar beet endophytic bacteria Achromobacter marplatensi s Growth factors of TNSC2011 CGMCC No. 8192

[0048] But should beet endophytic bacteria provided according to the present invention Achromobacter marplatensis TNSC2011 CGMCC No. 8192 was characterized to determine its specific growth factors, and the strain TNSC2011 was inoculated and cultivated. The results are shown in Table 2.

[0049] Table 2: Effects of temperature, pH, salt, and antibiotics on the growth of strain TNSC2011

[0050] temperature (°C) 4 15 25 30 32 35 growing situation — + + +++ ++++ +++ temperature (°C) 38 45 50 growing situation ++ - — pH 1 2 3 4 5 6 growing situation — — — — + ++ pH 7 8 9 10 growing situation +++ + — — NaCl concentration 0.5％ 1％ 2％ 3％ 4％ 5％ growing situation + ++ +++ +++ +++ + NaCl concentration 6％ 7％ 8％ 9％ ...

Embodiment 3

[0057] Embodiment three: sugar beet endophytic bacteria Achromobacter marplatensis Research on the fermentation process of TNSC2011 CGMCC No. 8192

[0058] The purpose of obtaining preparations by fermentation is achieved through the optimization of carbon, nitrogen sources, pH value, etc.

[0059] (1) Determination of medium pH

[0060] According to the pH value of 6.0, 6.5, 7.0, 7.5, 8.0, ferment at 120 r / min, temperature 35 ℃, the optimum growth is obtained.

[0061] (2) Optimization of carbon and nitrogen sources

[0062] Seed liquid medium: 2.0% corn flour, 0.5% yeast extract, 7.5% corn steep liquor, KH 2 PO 4 0.1%, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 00.05%, continue to optimize on the basis of seed culture medium;

[0063] A. Carbon source optimization:

[0064] The carbon sources are glucose, sucrose, corn flour, soluble starch, and other components are KH 2 PO 4 0.1%, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 O0.05% PH7.0;

[0065] B. Nitrogen source optimizatio...

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Abstract

The invention discloses achromobacter marplatensis TNSC2011with a preservation number of CGMCC (China General Microbiological Culture Collection) No.8192 and an application thereof in sugar beet cultivation. Taxonomic status of the obtained bacterial strain is primarily determined by carrying out determination and phylogenetic analysis of morphological characteristics, physiological-biochemical characteristics and a 16 SrDNA sequence on the obtained bacterial strain; meanwhile, characteristics such as bacteriostasis rate, growth promotion and stability of fermentation liquor of the bacterium TNSC2011 are deeply researched, so that crop growth can be effectively promoted; slightly-soluble phosphate can be converted into soluble phosphate which can be biologically utilized, so that photosynthesis is improved, and the achromobacter marplatensis is proved to be an excellent endophyte with the serial number of TNSC2011; the endophyte is applied on sugar beet cultivation to obtain a good effect, so that technical effect is outstanding and application value is wide.

Description

technical field [0001] The invention relates to the technical field of application of agricultural microorganisms, in particular to the technical field of endophytic growth-promoting bacteria of sugar beet and its application. Background technique [0002] Endophytes generally exist in plants, form a harmonious symbiont with the host, and produce a large number of secondary metabolic active substances. It is reported that endophytes can enhance the host's disease resistance, improve plant productivity, stress and insect resistance, and have herbicide activity and other characteristics. Therefore, endophytes can be used as potential factors in biological growth promotion, and have important uses in the development of ecological agriculture and biopesticides. plant endophytic bacteria ( Endophytic bactena ) can promote the growth of its host plants through different pathways, and its role is similar to that of plant rhizosphere bacteria ( PGPR ), plant growth-promoting bac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/025
Inventor 史应武高雁杨红梅张涛林青常玮李玉国欧提库尔玛合木提娄恺
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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