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Broad-spectrum antibacterial biological fertilizer

A biological fertilizer and broad-spectrum technology, applied in fertilization devices, fertilizer mixtures, applications, etc., to achieve easy results, broad-spectrum antibacterial advantages, and strong antibacterial effects

Inactive Publication Date: 2015-05-13
ZHANJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the single defect or deficiency of modern agricultural fertilizers and provide a broad-spectrum antibacterial biological fertilizer. The broad-spectrum antibacterial biological fertilizer is prepared by utilizing the immune protein synthesis ability of silkworm body. The preparation method is simple and fast. The production process is safe for humans and animals, low in cost, and non-polluting to the environment; the use of this broad-spectrum antibacterial biofertilizer can reduce the use of toxic pesticides, reduce agricultural production costs, and gradually restore soil ecology; make agriculture reasonable, safe, efficient, and high-yielding Virtuous circle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 adopts the following steps to prepare a kind of broad-spectrum antibacterial biological fertilizer, which is used to prevent bacterial wilt:

[0023] (1) Preparation of non-pathogenic R. solanacearum bacterial liquid of immune bioactive protein:

[0024] a Ralstonia solanacearum extraction and cultivation: take tomato, pepper, eggplant stem fluid from seriously ill plants, centrifuge at 1500~2000rp / min, take supernatant, inoculate 50g of rice medium per 50mL of supernatant, and store at room temperature in summer Under the condition of 80-90% humidity, shake culture at 500rpm / min in the dark until the logarithmic growth phase is observed under the microscope. When the OD560 value is 0.8-1, the bacterial liquid is centrifuged at 20000rpm / min, and The supernatant after centrifugation was stored for later use;

[0025] b. At room temperature, the humidity in the inoculation box is 75-80%. After detoxification, the fifth-instar silkworm is live, and the tail o...

Embodiment 2

[0028] Embodiment 2 adopts the following steps to prepare a kind of broad-spectrum antibacterial biological fertilizer, which is used to prevent Fusarium wilt of banana:

[0029] (1) Preparation of non-pathogenic Fusarium oxysporum solution of immune bioactive protein:

[0030] a The fungus liquid extruded from the pseudostem vascular bundles of bananas and rice bananas with severe "banana wilt" disease has been verified as Fusarium oxysporum f.sp.cubense (E.F.Smith) Snyder&Hansen. Cuban Specialist No. 1 physiological race and No. 4 physiological race were not separated and purified mixed bacteria; the rotting liquid was centrifuged at a speed of 20,000rp / min, and the supernatant of the pathogenic bacteria was extracted, and 500g of rice culture medium was inoculated with each 50mL supernatant. ~38°C, humidity 80~90%, culture in shading until the rice surface is covered with white long-haired Fusarium oxysporum mycelia, OD560=0.8~1 in the logarithmic growth phase of the myceli...

Embodiment 3

[0040] Embodiment 3 The present embodiment adopts the following raw materials to prepare a kind of broad-spectrum antibacterial biological fertilizer: a kind of broad-spectrum antibacterial biological fertilizer, it is characterized in that: it is prepared according to the following steps: merge 1kg immune bioactive protein non-pathogenic R. solanacearum liquid and 1kg of non-pathogenic Fusarium oxysporum liquid of immunobiologically active protein was mixed bacterial liquid; in the high-shear reaction kettle, 500 kg of clear water, 50 kg of crushed liquid of live silkworm living tissue pulverized by a tissue grinder, and mixed bacterial liquid were added successively. , Cytokinin: 2kg of 6-benzylaminoadenine, 2kg of gibberellin, 0.5kg of boric acid with a mass concentration of 0.1%, 0.5kg of manganese sulfate, 0.5kg of a sodium bicarbonate solution with a mass concentration of 0.5%, biological amplification at room temperature 72 hours, and then carry out stirring cultivation ...

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Abstract

The invention discloses a broad-spectrum antibacterial biological fertilizer. The broad-spectrum antibacterial biological fertilizer is composed of 0.5kg-2kg of immunobiologic active protein non-pathogenic ralstonia solanacearum liquid and / or 0.5kg-2kg of immunobiologic active protein non-pathogenic fusarium oxysporum schlecht liquid, 1kg-3kg of cytokinin, 1kg-5kg of phytohormone, 300g-700g of 0.1% boric acid, 300g-700g of manganese sulfate, 0.2kg-1.0kg of 0.5% sodium bicarbonate solution and 300kg-1000kg of clean water. A biological fertilizer product can be applied to more than 30 types of crops in a dilution ratio of 1:(800-1000) in a manner of root application or spraying so as to prevent bacterial wilt and wilt; expect the prevention of diseases, the yield can be increased by 10%-60%; the product has a strong antibacterial effect and efficient and broad-spectrum antibacterial advantages; according to the method, the technical route is simple and convenient; the product is stable and the production cost is low; the productivity controllability degree is high; no pollution is caused, a result is easy to convert and the broad-spectrum antibacterial biological fertilizer is convenient for industrial production.

Description

technical field [0001] The invention relates to a biological fertilizer, in particular to a broad-spectrum antibacterial biological fertilizer, which belongs to the technical field of agricultural production. Background technique [0002] Biological fertilizer in a narrow sense is a product that enables crops to obtain specific fertilizer effects through microbial life activities. It is also called inoculant or bacterial fertilizer. It does not contain nutrients and cannot replace chemical fertilizers. In a broad sense, biological fertilizers are products that contain both the nutrients needed by crops and microorganisms. They are a combination of biology, organic and inorganic substances. They can replace chemical fertilizers and provide various nutrients needed for crop growth and development. Due to the extensive use of chemical fertilizers and pesticides will have a certain impact on humans and the environment, therefore, in the development of "ecological agriculture" an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00
Inventor 罗富英李典
Owner ZHANJIANG NORMAL UNIV
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