Preparation method for hot pepper sauce
A technology of chili sauce and bacterial liquid, applied in food preparation, bacteria used in food preparation, and food ingredients as odor improvers, etc., can solve the problems of weak aroma of chili sauce fermentation, poor taste coordination, and limited antiseptic effect, and achieve Unique flavor, life extension, well tolerated effect
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Embodiment 1
[0039] The concrete breeding process of described plantarum lactobacillus is as follows:
[0040] 1. Diethyl Sulfate (DES) Mutation Breeding
[0041] (1) Take a ring of Lactobacillus plantarum on the inclined surface of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask containing 50mL liquid MRS xylan medium, and incubate at 200rpm at 40°C for about 12h, so that the bacteria are in the right condition. Early growth period.
[0042] (2) Take 5mL of bacterial liquid, centrifuge at 5000rpm for 10min to collect bacterial cells, and wash twice with normal saline.
[0043] (3) Dilute to 10 with pH7.0 phosphate buffer 7 individual / mL bacterial suspension.
[0044] (4) Take 32mL of pH7.0 potassium phosphate buffer, 8mL of bacterial suspension, and 0.4mL of DES in a 150mL Erlenmeyer flask pre-placed in the rotor and mix thoroughly so that the final concentration of DES is 1% (v / v).
[0045] (5) React in a shaker at 30°C at 150rpm for 30min, take 1mL of...
Embodiment 2
[0061] A preparation method of chili sauce, comprising the steps of:
[0062] (1) Preparation: Choose fresh American red or quinta red peppers with high red pigment content, remove impurities such as rotten peppers and pepper stems, and use a stainless steel grinder to crush them into 1cm-sized pepper particles for later use;
[0063] (2) Pickling: Put a layer of edible salt on the bottom of the fermentation tank, mix the chili sauce with sucrose, salt, and mixed bacterial liquid, and put it into the tank for fermentation. The sugar content of the prepared chili sauce is controlled at 0.75%, and the salt content is 0.75%. Control it at 5%, after the chili sauce is transferred into the pool, cover the surface with a layer of salt, and then seal it;
[0064] The mixed bacterial liquid is formed by mixing the bacterial liquid of Lactobacillus plantarum and the bacterial liquid of Lactobacillus rhamnosus;
[0065] The added amount of the Lactobacillus plantarum bacterium liquid i...
Embodiment 3
[0076] A preparation method of chili sauce, comprising the steps of:
[0077] (1) Preparation: Choose fresh American red or quinta red peppers with high red pigment content, remove impurities such as rotten peppers and pepper stems, and use a stainless steel grinder to crush them into 1cm-sized pepper particles for later use;
[0078] (2) Pickling: Put a layer of edible salt on the bottom of the fermentation tank first, mix the chili sauce with sucrose, salt, and mixed bacterial liquid, and put it into the tank for fermentation. The sugar content of the prepared chili sauce is controlled at 0.5%, and the salt content is 0.5%. Control it at 3%, after the chili sauce is transferred into the pool, cover the surface with a layer of salt, and then seal it;
[0079] The mixed bacterial liquid is formed by mixing the bacterial liquid of Lactobacillus plantarum and the bacterial liquid of Lactobacillus rhamnosus;
[0080] The added amount of the Lactobacillus plantarum bacterium liqu...
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