Preparation method of liquefied amylases capable of resisting superhigh temperature

A liquefying amylase, ultra-high temperature technology, applied in biochemical equipment and methods, microorganism-based methods, enzymes, etc., can solve the problems of high production cost, difficult temperature tolerance of amylase to exceed 100 ° C, containing and other problems

Inactive Publication Date: 2014-03-05
HUNAN HUXIANG BIOTECH CO LTD
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Problems solved by technology

[0005] The existing methods for producing high-temperature-resistant liquefaction amylase mainly have the following disadvantages: first, the production cost is relatively high; Most enterprises use ammoniu

Method used

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specific Embodiment approach

[0016] The present invention will be further described below in conjunction with examples, but these examples should not be construed as limiting the protection scope of the present invention. Without departing from the spirit and essence of the present invention, any modifications or replacements made to the method of the present invention belong to the protection scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and each raw material added is a commercially available product unless otherwise specified.

[0017] Step 1, agar medium inoculation culture: (1) Agar medium preparation, medium formula: tryptone 1.2g, yeast extract 0.6g, NaCl 1.0g, agar 2.0g, soluble starch 0.28g, pH6.8, the rest It is water, with a total weight of 100g; after mixing the 100g ingredients, put them in a petri dish, sterilize at 0.1MPa for 33min, cool to room temperature, and make an agar ...

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Abstract

The invention relates to a method for producing coenzymes Q10 via the fermentation of genetic recombinant bacteria. According to the method, NAD (Nicotinamide Adenine Dinucleotide) kinase genes are transferred into strains of reducing coenzymes Q10 with high-efficiency expression. The culture condition of optimizing the recombinant bacteria comprises (1) cant activation; (2) seed culture; and (3) fermentation culture. The recombinant bacteria constructed by a genetic engineering means are strong in stability; compared with original strains, the coenzymes Q10 of the recombinant bacteria has the advantages that the yield is increased by 21.6% and reaches 1.63g/L. Thus, the requirement of industrial production is satisfied.

Description

technical field [0001] The invention relates to a method for preparing amylase, in particular to a method for preparing amylase which still has relatively high activity at high temperature and is used for starch liquefaction. Background technique [0002] Starch is widely used in food, medicine, chemical industry, papermaking, petroleum and other fields, and its development prospects are very broad. With the continuous development of science and technology, the increasing number of new products such as starch, starch sugar and starch derivatives and the development of starch deep-processing products have accelerated and promoted the development of the starch industry. [0003] my country's starch and starch products industry is developing rapidly. The total starch output is increasing at an average annual rate of about 10-15%. It has become one of the largest starch producers in the world, laying a considerable material foundation for the development and production of starch...

Claims

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Application Information

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IPC IPC(8): C12N9/28C12R1/10
CPCC12N9/2411
Inventor 林亲录袁四岳孙术国吴跃梁盈付湘晋肖华西
Owner HUNAN HUXIANG BIOTECH CO LTD
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