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Natural plant preservative taking rape seed cakes and folium ginkgo as raw materials as well as preparation method and application thereof

A technology of natural plants and rapeseed cakes, applied in the field of phytochemistry, can solve problems such as adverse effects on the natural ecological environment, chemical preservatives, threats to human health, etc., and achieve the effects of low cost, extended shelf life, and low price

Inactive Publication Date: 2014-03-05
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main preservatives used in the food industry are still chemical preservatives, including benzoic acid and its sodium salt, sorbic acid and its salts, calcium propionate, nitrate and nitrite, etc., but these preservatives are harmful to animals and humans. Has a certain toxic effect. Chemical preservatives have potential shock-causing, teratogenic and carcinogenic effects, which pose a greater threat to human health and also cause adverse effects on the natural ecological environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Dry the rapeseed cake and ginkgo leaves after black rapeseed oil extraction at 40-50°C for 2 days, take 700g of rapeseed cake and 300g of ginkgo leaves, mix well, grind them into powder, pass through a 40-mesh sieve, Have to mix the powder and set aside. At room temperature, use ethanol with a pH value of 1.0 and a concentration of 70% as the extraction solvent, put the mixed powder and the extraction solvent into the extraction container at a mass ratio of 1:12; ultrasonicate in an ultrasonic instrument at 600W and 40°C for 60 minutes, and then Use 600W microwave power to irradiate for 60s, filter and retain the filtrate; extract the filter residue once more in the same way, and combine the filtrate after the end. Concentrate in vacuum at 0-50°C to obtain solid powder; the solid powder is dried and crushed to 150 mesh to obtain rapeseed cake and ginkgo leaf extract, which are natural plant preservatives.

Embodiment 2

[0014] Dry the rapeseed cake and ginkgo leaves after black rapeseed oil extraction at 40-50°C for 2 days, take 750g of rapeseed cake and 250g of ginkgo leaves, mix well, grind them into powder, pass through a 40-mesh sieve, Have to mix the powder and set aside. At room temperature, use ethanol with a pH value of 1.5 and a concentration of 65% as the extraction solvent, put the mixed powder and the extraction solvent into the extraction container at a mass ratio of 1:10; ultrasonicate for 40 minutes at 500W and 45°C in an ultrasonic instrument, and then use Irradiate under 500W microwave power for 40s, filter and retain the filtrate; extract the filter residue once more according to the same method, and combine the filtrate after the end. Concentrate in vacuum at 0-50°C to obtain solid powder; the solid powder is dried and crushed to 150 mesh to obtain rapeseed cake and ginkgo leaf extract, which are natural plant preservatives.

Embodiment 3

[0016] Dry the rapeseed cake and ginkgo leaves after black rapeseed oil extraction at 40-50°C for 3 days, take 800g of rapeseed cake and 200g of ginkgo leaves, mix well, grind them into powder, pass through a 40-mesh sieve, Have to mix the powder and set aside. At room temperature, use ethanol with a pH value of 2.0 and a concentration of 75% as the extraction solvent, put the mixed powder and the extraction solvent into the extraction container at a mass ratio of 1:15; ultrasonicate for 30 minutes at 400W and 50°C in an ultrasonic instrument, and then use Irradiate under 400W microwave power for 30s, filter and retain the filtrate; extract the filter residue twice by weight according to the same method, and combine the filtrate after the end. Concentrate in vacuum at 0-50°C to obtain solid powder; the solid powder is dried and crushed to 150 mesh to obtain rapeseed cake and ginkgo leaf extract, which are natural plant preservatives.

[0017] Second, the antibacterial effect ...

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PUM

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Abstract

The invention relates to a natural plant preservative taking rape seed cakes and folium ginkgo as raw materials as well as a preparation method and an application thereof. The natural plant preservative is an extract of the rape seed cakes and the folium ginkgo. The preparation method comprises the following steps: drying the rape seed cakes and the folium ginkgo; mixing 70 to 80 parts by mass of the rape seed cakes with 20 to 30 parts by mass of the folium ginkgo; crushing and sieving; then placing the mixture in an extraction container; performing extraction by using an ultrasonic wave and microwave dual-assisted method under the extraction conditions of performing ultrasonic treatment for 30 to 60 minutes at 40 DEG C to 50 DEG C and under the ultrasonic power of 400W to 600W and then radiating for 30 to 60 seconds under the microwave power of 400W to 600W by taking ethyl alcohol with a pH value of 1.0 to 2.0 and the concentration of 65% to 75% as an extraction solvent according to the solid-to-liquid ratio being 1:(10-15); and filtering and concentrating so as to obtain the extract. The extract can be used for inhibiting the growth and the breeding of staphylococcus aureus, escherichia coli, mucedine and the like. The preservative provided by the invention is completely prepared from natural plants, so that the preservative cannot generate toxic and side effects and is wide in antimicrobial spectrum. Thus, the preservative can be widely applied to the fields of foods and feeds.

Description

technical field [0001] The invention belongs to the field of phytochemistry, and relates to a preparation method and application of a plant extract, in particular to a natural plant preservative capable of inhibiting or killing saprophytic organisms, namely the extraction of rapeseed cake and ginkgo leaf extract method and its use. Background technique [0002] One of the key issues in food processing based on agricultural products is the antiseptic preservation of food. In order to prolong the preservation period of food, adding preservatives is a convenient preservation method. At present, the main preservatives used in the food industry are still chemical preservatives, including benzoic acid and its sodium salt, sorbic acid and its salts, calcium propionate, nitrate and nitrite, etc., but these preservatives are harmful to animals and humans. There is a certain toxic effect. Chemical preservatives have potential shock-causing, teratogenic and carcinogenic effects, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3463
CPCA23L3/3463
Inventor 严明理刘丽莉黄婷丁素萍杨俊
Owner HUNAN UNIV OF SCI & TECH
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