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Combination of EPA, DPA and/or DHA with a chemotherapeutic agent

A chemotherapeutic agent, inhibitor technology, applied in the field of combined use of EPA, DPA and/or DHA and chemotherapeutic agents, can solve the problem of no disclosure of EPA and the like

Inactive Publication Date: 2014-02-26
NV NUTRICIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, none of these documents disclose specific concentrations of EPA, DPA and / or DHA with high efficacy in the prevention and / or treatment of neoplastic diseases

Method used

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  • Combination of EPA, DPA and/or DHA with a chemotherapeutic agent
  • Combination of EPA, DPA and/or DHA with a chemotherapeutic agent
  • Combination of EPA, DPA and/or DHA with a chemotherapeutic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Total Fatty Acid Analysis

[0098] Murine C26 adenocarcinoma cells were routinely cultured twice a week, and cell cultures were studied with binoculars to determine cell confluency. The medium was removed with a vacuum aspirator and the cells were washed once with 10 ml of pre-warmed PBS. PBS was removed and 2 ml of trypsin-EDTA was added to the culture flask. After a short incubation in the incubator, cells were studied with binoculars to determine cell detachment. The cells were thoroughly resuspended in 8 ml medium. Cells were passaged in new flasks at a ratio of 1:3 or 1:5, depending on the confluence of the cell culture. Additionally, 25 ml of fresh medium was added to the bottle.

[0099] To determine total phospholipid fatty acids, murine C26 cells were cultured in 6-well plates. After 4 days of incubation with 50 μM of EPA, DPA or DHA, cells were washed with pre-warmed PBS and trypsinized. After detachment, cells were resuspended in 1 ml medium and transfe...

Embodiment 2

[0102] Metabolic activity of EPA, DHA or DPA

[0103] C26 cells were pre-incubated with EPA, DHA or DPA for 4 days prior to chemotherapy incubation at a final concentration of 50 μM. To avoid direct interaction between fatty acids and chemotherapeutic drugs, media containing fatty acids was removed prior to the addition of chemotherapeutic drugs. Cells were incubated with the chemotherapeutic drugs cisplatin and doxorubicin for 24 hours, and their metabolic activity was measured after incubation. Figure 4 shows the results of metabolic activity of chemotherapy-treated cells pre-incubated with EPA, DHA or DPA. Addition of EPA or DPA resulted in a significant decrease in metabolic activity after cisplatin treatment (p<0.0001) as well as after doxorubicin treatment (p<0.0001). DHA did not show significant changes in metabolic activity compared to controls.

Embodiment 3

[0105] skeletal muscle function

[0106] 5–6 week-old male CD2F1 mice (BALB / c x DBA / 2, Harlan / Charles River, The Netherlands) were housed individually in a climate-controlled room (constant 21±1°C room temperature, 12:12 light-dark cycle). After one week of acclimation, the mice were divided into weight-matched groups: (1) the control group received the control diet (C), (2) the tumor-bearing group received the control diet (TB) and (3) the tumor-bearing group received the same as before The Specific Nutrient Combination (TB-SNC) diet containing high protein, leucine and fish oil content as active ingredients with the addition of a specific oligosaccharide mixture [1]. All experimental procedures were approved by the animal ethics committee (DEC consultants, Bilthoven, The Netherlands) and followed the principles of good laboratory animal care.

[0107] The muscle contractile properties of the extensor digitorum longus (EDL) muscle of 7–9-week-old male CD2F1 mice were evaluat...

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Abstract

The present invention refers to a combination of an eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and / or docosahexanoic acid (DHA), or a combination of a nutritional composition comprising EPA, DPA, and / or DHA and a protein, a carbohydrate, a fat, an amino acid, a fatty acid, a dietary fibre, a vitamin, a mineral, a trace element, ß-carotenoid, a flavonoid, a nucleotide, L- carnitin, choline, inositol, taurine, creatine, and / or a co-enzyme, wherein EPA is in an amount of 1 to 1000 mg / 100ml, preferably 100 to 700 mg / 100ml, DPA is in an amount of >50 mg / 100ml, preferably 50 to 1000 mg / 100ml, preferably 6 to 800 mg / 100ml, more preferably 80 to 500 mg / 100ml and / or DHA is in an amount of 1 to 500 mg / 100ml, preferably 1 to 300 mg / 100ml and a chemotherapeutic agent selected from the group consisting of an alkylating drug, an antimetabolite, an antimytotic cytostatic, a topoisomerase inhibitor, antitumor antibiotic, and any other cytostatic, and / or a radiotherapy. These combinations are successful for use in treating a neoplastic disease like the method for treating a neoplastic disease comprising EPA, DPA, and / or DHA or a nutritional composition comprising EPA, DPA, and / or DHA and and a protein, a carbohydrate, a fat, an amino acid, a fatty acid, a dietary fibre, a vitamin, a mineral, a trace element, ß-carotenoid, a flavonoid, a nucleotide, L- carnitin, choline, inositol, taurine, creatine, and / or a co-enzyme in combination with a chemotherapeutic agent, and / or radiotherapy.

Description

technical field [0001] The present invention relates to eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and / or docosahexaenoic acid (DHA), and EPA, DPA and / or DHA and proteins, carbohydrates , lipids, amino acids, fatty acids, dietary fiber, vitamins, minerals, trace elements, β-carotenoids, flavonoids, nucleotides, L-carnitine, choline, inositol, taurine, creatine and / or coenzymes, respectively, in combination with chemotherapeutic agents for the treatment of neoplastic diseases. The present invention further relates to a method for treating tumor diseases, said method comprising administering EPA, DPA and / or DHA or EPA, DPA and / or DHA and proteins, carbohydrates, lipids, amino acids, fatty acids, dietary fibers, Vitamins, minerals, trace elements, beta-carotenoids, flavonoids, nucleotides, L-carnitine, choline, inositol, taurine, creatine and / or coenzymes. The invention further relates to a composition comprising or consisting of EPA or DPA, preferably for the tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/035
CPCA61K45/06A61K31/035A61P35/00A61K2300/00A61K31/202
Inventor F·J·迪杰克K·范诺伦M·范迪杰克-奥滕斯
Owner NV NUTRICIA
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