Cotton ghmate1 gene and its application in improving the color of cotton brown fiber

A brown fiber and gene technology, applied in the field of plant expression of binary vectors and in improving the color of cotton brown fiber, can solve the problems of low clothing content, limited application of brown cotton, low fiber specific strength, etc.

Inactive Publication Date: 2015-10-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Compared with white cotton, colored cotton varieties still have the following outstanding problems: low fiber specific strength, short fiber length, low clothing content, poor pigment stability, and single color, which limits the application of brown cotton in the textile industry

Method used

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  • Cotton ghmate1 gene and its application in improving the color of cotton brown fiber
  • Cotton ghmate1 gene and its application in improving the color of cotton brown fiber
  • Cotton ghmate1 gene and its application in improving the color of cotton brown fiber

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Embodiment Construction

[0030] 1. Materials and methods

[0031] 1.1 Plant material and growing conditions

[0032] Upland cotton brown fiber Zhejiang cotton No. 2, white fiber cotton Zhejiang cotton 11, diploid brown fiber cotton Cixi purple cotton and white fiber cotton Yuyao Chinese cotton are all produced by the Economic Crops Research Office of the Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences Provided and sown live in the experimental field of the headquarters of the Hangzhou Institute of the hospital in early May 2011. On the 9th day after cotton flowering (DPA, Day Post Anthesis), 12d, 15d, 18d, 21d and 24d after flowering, the developing cotton fiber tissue and seed coat tissue were selected to carry out related research.

[0033] 1.2 Experimental method

[0034] 1.2.1 Extraction of cotton genomic DNA and total RNA from cotton fiber and seed coat tissue

[0035] Cotton genomic DNA was extracted by CTAB method (Li et al., 2001). The total...

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Abstract

The invention relates to an improved cotton fiber color gene technology, and particularly relates to a cotton gene and a plant expression binary vector constructed by the gene and application to improvement of cotton brown fiber color. A nucleotide sequence of the cotton GhMATE1 gene is shown in SEQ ID: NO.1. An amino acid sequence of a protein coded by the gene is shown in SEQ ID: NO.2. On the basis of an early-cloned cotton GhTT12a gene (Chinese patent application number: 201310380270.5, and data of application: 2013-8-27), by using a bioinformatics analysis and electronic cloning method, a new gene containing an MATE (multidrug and toxic compound extrusion) conserved domain database is cloned, but a gene structure and a shearing mode of the cotton GhMATE1 gene are completely different from those of the GhTT12a gene, and the cotton GhMATE1 gene has 78 percent of sequence homology with a nucleotide sequence of an arabidopsis thaliana testa brown pigment synthetic TT12 (Transparent Testa) gene.

Description

technical field [0001] The invention relates to a technology for improving cotton fiber color and luster, in particular to a cotton gene and a plant expression binary vector constructed thereof and its application in improving the color and luster of cotton brown fibers. Background technique [0002] Natural colored cotton is a special type of cotton whose cotton fiber has natural color. Because of its unique natural color, it does not need to be dyed in the process of spinning and processing. It is non-polluting to humans and the environment, and is comfortable to wear. It is especially suitable for making close-fitting clothing. The real "ecological" and "environmentally friendly" cotton has attracted widespread attention from domestic and foreign cotton breeders and the textile and clothing industries, and is known as the most potential ecological textile in the international market in the 21st century. Although the research and development of colored cotton in China star...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/84C12N1/21C07K14/415A01H5/00
Inventor 李付振祝水金邱新棉吴学龙王美兴
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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