Original ecological imitative wild cultivation method for inonotus sanghuang

A technology imitating wild cultivation and Phellinus japonica, applied in botany equipment and methods, horticulture, application, etc., can solve problems such as difficult large-scale cultivation, impact on the quality of Phellinus japonica, and harsh cultivation conditions, so as to reduce production costs and The effects of labor intensity, stable mulberry area, and simple cultivation methods

Inactive Publication Date: 2015-04-29
CHUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, due to the particularity and complexity of the physiological state and the constraints of the external environment, it is difficult for Phellinus to form fruiting bodies in nature, resulting in a very small number of natural Phellinus, limited medicinal resources in nature, and domestic and foreign The market demand for Phellinus is increasing, resulting in the predatory collection of medicine farmers in various domestic production areas, fruiting bodies cannot be formed in large quantities, and it is difficult to become a stable source of industrial products, which is very unfavorable to the development of Phellinus industry, so artificial cultivation is used to make up for it The shortage of wild resources is particularly important
However, the artificial cultivation of Phellinus Phellinus is extremely difficult, and there are problems of harsh cultivation conditions and long growth cycle.
[0004] There are mainly two kinds of artificial cultivation currently cultivated, one is based on the section wood cultivation of mulberry, poplar, and oak, which requires a large amount of wood resources, and the waste of resources is serious, so it is difficult to cultivate on a large scale; the other It is bag material cultivation, but there are problems such as insufficient nutrient supply of the medium, small fruit body development, easy to be infected with miscellaneous bacteria, etc.
The Chinese patent application number 200410041704.X discloses a method for artificially high-yield cultivation of Phellinus phellidis and the application of Phellinus radix fruiting body. The invention adopts bag material cultivation. In order to solve the problem of insufficient nutrient supply of the medium, the fruiting body is less developed and easy to grow. Bacteria and other issues
Auxin is added to the medium, but the addition of auxin does not fundamentally solve the problem of the medium, and the addition of auxin has an impact on the quality of Phellinus, and is not conducive to the natural growth of Phellinus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Isolation of Phellinus bacterium and cultivation of Phellinus mother species

[0020] Phellinus fruiting bodies with large flowers, thick flesh, bright yellow gills, and no pests and diseases were selected from mulberry trees as the separation material. After rinsing and disinfecting the surface of Phellinus fruiting body, cut the fruiting body under aseptic conditions, pick about 0.5 cm square tissue pieces in the middle layer of the fruiting body and place them on the mother seed medium plate at a certain distance, each plate Six pieces were cultured at about 28°C. After 24 hours, fine and fine hairy hyphae grew on the tissue pieces. Use an inoculation knife to transfer the apex mycelium into the mother medium, and culture at about 25°C for 10 days. Mycelia covered the slope.

[0021] If mushy viscous colonies or fast-growing fungal colonies are found, the tubes will not be transferred and re-separation is required. Phellinus strains can also be separated by spo...

Embodiment 2

[0042] Concrete method is the same as embodiment 1, and difference is: The mother seed medium adopts the following formula: 50 g of mulberry branches, 20 g of fresh bran, 200 g of potatoes, 20 g of glucose, 18 g to 23 g of agar, 1.5 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, and 0.5 g of magnesium sulfate.

[0043] Preparation method of mother seed medium: Weigh the required raw materials according to the formula, add 500-600ml of water to mix the mulberry twig scraps and fresh bran to boil the juice, filter with gauze, and get the filtrate. Peel the potatoes, dig out the bud eyes, and slice. After the potato slices are boiled, filter them with gauze, add the filtrate to agar and glucose, heat until the agar dissolves, mix with the mulberry twigs and fresh bran filtrate prepared above, and make up to 1000ml of water to adjust the pH to 5-6.5. Divide into test tubes while hot. The medium capacity of each test tube accounts for about 1 / 5 of the height of t...

Embodiment 3

[0045] The specific method is the same as in Example 1, the difference is that the wooden nail culture medium has a formula by weight: 45% of mulberry branch, 20% of mulberry sawdust, 16.6% of bran, 16% of corn flour, 1.2% of sugar, carbonic acid Calcium 1.2%, plus ammonium sulfate 0.5%. The diameter of the mulberry branch is 0.7 centimeters, 4 centimeters long, and one end is pointed.

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Abstract

The present invention relates to the field of biotechnology, and specifically relates to an original ecological imitation wild cultivation method of Phellinus phellidis. (2) The original Phellinus seed was inoculated on the original seed medium to obtain the original Phellinus seed; (3) The original Phellinus seed was inoculated into the wooden nail medium in the cultivation bag to obtain the (4) The wooden nail cultivar covered with Phellinus mycelium is inoculated on the living body of mulberry tree for natural cultivation. The present invention obtains Inonotus by separating and purifying wild Phellinus? sanghuang ( Phellinus ) strains, and then directly inoculate the strains on the live mulberry trees in the mulberry garden, and use the live mulberry trees as the growth host of Phellinus sanghuang to artificially increase the probability of the mulberry trees being infected with Phellinus sphaerifolia. Wild similar, higher medicinal value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an original ecological imitation wild cultivation method of Phellinus japonica. Background technique [0002] Sanghuang, also known as Sangchen, Sanger, Husunyan, and Phellinus. "Shen Nong's Materia Medica" records its medicinal efficacy: "strengthening the five internal organs, clearing the stomach qi, and detoxifying qi." According to modern medical research data, Phellinus is currently the first internationally recognized biological cancer treatment agent in terms of effectiveness. Rare medicinal fungi. Phellinus belongs to Basidiomycetes, Polyporacea, Polyporaceae, and Phellinus, and is a precious perennial large medicinal fungus. Phellinus linteus (Phellinus igniarius), Phellinus igniarius (Phellinus igniarius), Phellinusbaumii (Phellinus baumii) and other scientific names have been used successively in the literature reports. After 6 years of research, Wu Shenghua, Dai Yuche...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 郑社会余建妹王伟科袁卫东
Owner CHUNAN INST OF MICROBIOLOGY
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