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Method for measuring content of calycosin-7-glucoside in Zhenqifuzheng preparation

A technology of acteocoside and Zhenqi Fuzheng, which is applied in the field of quality inspection of traditional Chinese medicine, and can solve problems such as content determination of acteocoside components

Active Publication Date: 2014-02-19
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing quality inspection methods for Zhenqi Fuzheng preparations do not measure the content of actcoisoflavone glycosides. In order to further improve the quality inspection standards of Zhenqi Fuzheng preparations and ensure the quality of their drugs, the establishment of a method for the determination of the content of acteosin in Zhenqi Fuzheng preparations. The content determination project of flavonoid glycosides is very necessary

Method used

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  • Method for measuring content of calycosin-7-glucoside in Zhenqifuzheng preparation
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  • Method for measuring content of calycosin-7-glucoside in Zhenqifuzheng preparation

Examples

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Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 of the present invention: A method for determining the content of Verbasil glycosides in Zhenqi Fuzheng preparations was carried out according to the following steps:

[0077] (1) Chromatographic conditions and system suitability test: octadecylsilane-bonded silica gel is used as filler; acetonitrile: 0.2% formic acid aqueous solution=20:80 as mobile phase; detection wavelength is 260nm; flow rate is 1.0mL / min ; The column temperature is 35℃; the number of theoretical plates should not be less than 3000 according to the peak of the mullein isoflavone glycoside;

[0078] (2) Preparation of reference solution: accurately weigh out an appropriate amount of verbasil glycoside reference substance, add methanol to make a 0.1mg / mL solution, and set aside;

[0079] (3) Preparation of test solution: accurately weigh 2.0g of Zhenqi Fuzheng preparation or its contents, accurately add 100mL of 70%-75% ethanol, weigh it, extract it with ultrasound for 1h, make up the weight with ...

Embodiment 2

[0081] Example 2 of the present invention: A method for determining the content of Verbasil glycosides in Zhenqi Fuzheng preparations was performed according to the following steps:

[0082] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica as filler; use acetonitrile:0.05% glacial acetic acid=30:70 as mobile phase; detection wavelength is 260nm; flow rate is 0.8mL / min ; The column temperature is 35℃; the number of theoretical plates should not be less than 3000 based on the peak of mullein isoflavone glycoside;

[0083] (2) Preparation of reference solution: accurately weigh out an appropriate amount of verbasil glycoside reference substance, add methanol to make a 0.1mg / mL solution, and set aside;

[0084] (3) Preparation of test solution: accurately weigh 2.0 g of Zhenqi Fuzheng preparation or its contents, accurately add 100 mL of methanol, weigh it, and extract with ultrasound or reflux for 1 hour, make up the weight with methanol, fil...

Embodiment 3

[0086] Example 3 of the present invention: A method for determining the content of Verbasil glycosides in Zhenqi Fuzheng preparations was performed according to the following steps:

[0087] (1) Chromatographic conditions and system suitability test: use octadecylsilane-bonded silica gel as filler; use methanol:0.05% phosphoric acid aqueous solution=40:60 as mobile phase; column temperature is 35℃; detection wavelength is 275nm; The flow rate is 0.9mL·min -1 ;The number of theoretical plates should not be less than 3000 based on the peak of mullein isoflavone glycoside;

[0088] (2) Preparation of reference solution: accurately weigh out an appropriate amount of verbasil glycoside reference substance, add methanol to make a 0.1mg / mL solution, and set aside;

[0089] (3) Preparation of test solution: accurately weigh 2.0 g of Zhenqi Fuzheng preparation or its contents, accurately add 100 mL of methanol, weigh it, and extract with ultrasound or reflux for 1 hour, make up the weight wit...

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Abstract

The invention discloses a method for measuring content of calycosin-7-glucoside in a Zhenqifuzheng preparation. The method is a high-performance liquid chromatography with a calycosin-7-glucoside reference substance used as a contrast and the ratio of methyl alcohol to water / a 0.01%-5% phosphoric acid aqueous solution / a 0.01%-5% glacial acetic acid / a 0.01%-0.5% formic acid aqueous solution of (30-70):(70-30) or the ratio of acetonitrile to the water / the 0.01%-5% phosphoric acid aqueous solution / the 0.01%-5% glacial acetic acid / the 0.01%-0.5% formic acid aqueous solution of (20-50):(80-50) used as a mobile phase. The method is high in specificity, high in accuracy, good in repeatability, high in recovery rate, high in stability, accurate in measurement result and capable of achieving the purpose of effective control of the drug quality and ensuring the stability of the product quality and the safety and the effectiveness of clinical medication.

Description

Technical field [0001] The invention relates to a method for determining the content of Verbasil glycosides in Zhenqi Fuzheng preparations, and belongs to the technical field of quality detection of traditional Chinese medicines. Background technique [0002] Zhenqi preparations have the effects of invigorating qi, nourishing yin, and strengthening the body. Modern pharmacological experiments have proved that it can improve human immune function, protect bone marrow and adrenal cortex function, and promote interferon production; cooperate with tumor patients with radiotherapy and chemotherapy, reduce the side effects of patients during radiotherapy and chemotherapy, and promote the recovery of normal function. The prescription consists of Astragalus and Ligustrum lucidum. At present, the dosage forms of Zhenqi preparations for clinical choice include tablets, capsules, granules and dripping pills. [0003] The main active ingredients in astragalus are saponins and flavonoids. Stu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张观福
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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