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Method for detecting glyceryl monostearate in liquid milk

A technology of glycerol monostearate and liquid milk, which is applied in measuring devices, instruments, scientific instruments and other directions to achieve the effects of high accuracy and easy operation

Inactive Publication Date: 2014-02-12
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of a unified detection method specifically for glyceryl monostearate in liquid milk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0017] The test parameters of the gas chromatographic value in this embodiment are: splitless sampling; the temperature of the gasification chamber is 300 ° C; the chromatographic column is a VF-5 capillary column, and the specification is 30m × 0.25mm × 0.25um; the temperature is programmed, and the initial temperature is 120°C, hold for 1min, raise to 290°C at 10°C / min, hold for 15min; detector temperature is 320°C; carrier gas is nitrogen, flow rate is 1.5mL / min; injection volume is 1uL.

[0018] 1. Operation steps.

[0019] a. Extract the milk fat in the pure milk to be tested by using the Gothic-Luozi method, specifically: take 2.0g of the pure milk to be tested and place it in a colorimetric tube, add 1.0mL of ammonia water in turn, shake gently, add 4mL of 95% ethanol, Add 6 mL of diethyl ether, shake 50 times, add 6 mL of petroleum ether, shake 50 times, and wash the bottle cap with a mixture of diethyl ether-petroleum ether with a volume ratio of 1:1 to obtain an extr...

no. 2 example

[0039] The test parameters of the gas chromatographic value in this embodiment are the same as those described in the first embodiment.

[0040] 1. Operation steps.

[0041]a. Extract the milk fat in the fruit-flavored milk to be tested by using the Gottlie-Luozi method, specifically: take 2.0g of the fruit-flavored milk to be tested and put it in a colorimetric tube, add 0.8mL of ammonia water in turn, shake gently, and add 3mL of 95% Ethanol, add 5mL ether, shake 50 times, add 5mL petroleum ether, shake 50 times, and wash the bottle cap with a mixture of ether-petroleum ether with a volume ratio of 1:1 to obtain an extract; let the extract stand for 20 Minutes later, the organic phase was separated and dried with nitrogen in a water bath at 45°C to obtain the milk fat to be tested.

[0042] b. Weigh 0.1g 1-glyceryl palmitate and 0.1g 1-glyceryl stearate, place in a 10mL volumetric flask, and use n-hexane to make up to the mark to prepare the standard solution mother liquor ...

no. 3 example

[0061] The test parameters of the gas chromatographic value in this embodiment are the same as those described in the first embodiment.

[0062] 1. Operation steps.

[0063] a. Use the Gothic-Luo Zi method to extract the milk fat in the high-calcium milk to be tested, specifically: take 2.0g of the high-calcium milk to be tested and put it in a colorimetric tube, add 1.2mL of ammonia water in turn, shake gently, and add 5mL of 95% Ethanol, add 7mL ether, shake 50 times, add 7mL petroleum ether, shake 50 times, and wash the bottle cap with a mixture of ether-petroleum ether with a volume ratio of 1:1 to obtain an extract; let the extract stand for 20 Minutes later, the organic phase was separated and dried with nitrogen in a water bath at 55° C. to obtain the milk fat to be tested.

[0064] b. Weigh 0.1g 1-glyceryl palmitate and 0.1g 1-glyceryl stearate, place in a 10mL volumetric flask, and use n-hexane to make up to the mark to prepare the standard solution mother liquor of ...

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Abstract

The invention provides a method for detecting glyceryl monostearate in liquid milk. The method comprises the following steps: a. preparing a bottle of liquid milk to be tested, extracting milk fat from the liquid milk so as to obtain milk fat to be tested; b. preparing a standard solution of glyceryl monostearate, blow-drying the standard solution with nitrogen gas so as to obtain a reference substance; c. separately adding N,O-bis(trimethylsilyl)trifluoroacetamide into the milk fat to be tested and the reference substance to carry out derivatization reactions so as to separately obtain a derivative sample and a derivative reference substance; d. blow-drying the derivative sample and the derivative reference substance with nitrogen gas, then separately dissolving the derivative sample and the derivative reference substance with n-hexane so as to separately obtain a sample liquid to be tested and a reference detection liquid; e. measuring the gas chromatography values of glyceryl monostearate in the sample liquid to be tested and the reference detection liquid, and calculating according to the gas chromatography values of glyceryl monostearate in the sample liquid to be tested and the reference detection liquid so as to obtain the content of glyceryl monostearate in the liquid milk to be tested. The detection method has the advantages of convenient operation and high accuracy.

Description

technical field [0001] The invention relates to a method for detecting glyceryl monostearate in liquid milk, in particular to a method for detecting the content of glyceryl monostearate in liquid milk by using a gas chromatograph. Background technique [0002] Glyceryl monostearate is milky white to light yellow powder or waxy solid, odorless, tasteless, insoluble in water, but soluble in ethanol and hot fat oil. Glyceryl monostearate is widely used in a variety of food processing. The main components are 1-glyceryl palmitate and 1-glyceryl stearate. In the field of detection, 1-glyceryl palmitate and 1-stearin are also commonly used The sum of the fatty acid glyceride content represents the content of glyceryl monostearate. Adding glyceryl monostearate to dairy products can promote milk fat to disperse more uniformly and stably in water, prevent quality problems such as fat floating, and at the same time, because of its good antioxidant effect, it can also improve the stor...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 姜瑞清陈军周琳常建军宋晓东
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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