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Method for measuring content of oleanolic acid in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation

A technology of oleanolic acid and Zhenqi Fuzheng, which is applied in the field of content determination of oleanolic acid in Zhenqi Fuzheng preparations, and can solve the problems of inability to form quality inspection standards and undisclosed oleanolic acid content determination, etc.

Inactive Publication Date: 2014-02-05
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent No. 200710077983.9, titled "A Method for Quality Control of Zhenqi Fuzheng Traditional Chinese Medicine Preparation for Prolonged Illness and Deficiency of Qi and Yin", discloses a method for identifying oleanolic acid in Zhenqi Fuzheng preparation , but it does not disclose how to determine the content of oleanolic acid in Zhenqi Fuzheng preparation, so it cannot form a set of strict and reliable quality inspection standards

Method used

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  • Method for measuring content of oleanolic acid in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation
  • Method for measuring content of oleanolic acid in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation
  • Method for measuring content of oleanolic acid in glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation

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Effect test

Embodiment 1

[0067] Determination of the content of oleanolic acid in Zhenqi Fuzheng Capsules (prepared according to the method described in the example of invention patent 200310122216.7):

[0068] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; acetonitrile: 0.05% phosphoric acid aqueous solution = 85:15 is used as mobile phase; flow rate is 0.8-1.0mL min -1 ;The detection wavelength is 215nm; the column temperature is 25°C; the number of theoretical plates calculated based on the oleanolic acid peak should not be less than 1000;

[0069] (2) Preparation of reference substance solution: Take an appropriate amount of oleanolic acid reference substance, weigh it accurately, add methanol to make 1 mg / mL oleanolic acid reference substance solution;

[0070] (3) Preparation of the test solution: Accurately weigh 14g of the contents of Zhenqi Fuzheng Capsules, accurately add 100mL of methanol, weigh, ultrasonically extract for 50...

Embodiment 2

[0073] Determination of the content of oleanolic acid in Zhenqi Fuzheng tablet (prepared according to the method described in the example of invention patent 200310122216.7):

[0074] (1) Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel is used as filler; methanol: 5% phosphoric acid aqueous solution = 85:15 is used as mobile phase; flow rate is 1.5mL min -1 ;The detection wavelength is 220nm; the column temperature is 40°C; the number of theoretical plates calculated based on the peak of oleanolic acid should not be less than 1000;

[0075] (2) Preparation of reference substance solution: Take an appropriate amount of oleanolic acid reference substance, weigh it accurately, add methanol to make 1 mg / mL oleanolic acid reference substance solution;

[0076] (3) Preparation of the test solution: Accurately weigh 14g of Zhenqi Fuzheng tablet, accurately add 100mL of methanol, weigh the weight, ultrasonically extract for 60min, make up the ...

Embodiment 3

[0079] Determination of the content of oleanolic acid in Zhenqi Fuzheng Granules (prepared according to the method described in the example of invention patent 200310122216.7):

[0080] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel is used as filler; methanol: 0.01% phosphoric acid aqueous solution = 95:5 is used as mobile phase; flow rate is 0.5mL min -1 ; The detection wavelength is 200nm; the column temperature is 20°C; the number of theoretical plates calculated based on the peak of oleanolic acid should not be less than 1000;

[0081] (2) Preparation of reference substance solution: Take an appropriate amount of oleanolic acid reference substance, weigh it accurately, add methanol to make 1 mg / mL oleanolic acid reference substance solution;

[0082] (3) Preparation of the test solution: Accurately weigh 14g of Zhenqi Fuzheng Granules, accurately add 100mL of methanol, weigh the weight, ultrasonically extract for 30 minutes, ...

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Abstract

The invention discloses a method for measuring the content of oleanolic acid in a glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation. The method takes an oleanolic acid reference substance as a reference and can be used for measuring the content of the oleanolic acid in the glossy privet fruit and astragalus membranaceus healthy energy-strengthening preparation at the wavelength being 200-220nm by using a high-performance liquid chromatography. The method is high in specificity and precision, good in repeatability, high in recovery rate and stability and accurate in measurement result, and achieves the purpose of effectively controlling the quality of drugs so as to ensure the stable product quality and the safe and effective clinical treatment.

Description

technical field [0001] The invention relates to a method for determining the content of oleanolic acid in Zhenqi Fuzheng preparation, which belongs to the technical field of traditional Chinese medicines. Background technique [0002] Zhenqi preparations have the effects of invigorating qi and nourishing yin, strengthening the body and strengthening the foundation. Modern pharmacological experiments have proved that Zhenqi preparation can improve human immune function, protect bone marrow and adrenal cortex, and promote the production of interferon; in addition, it can also be used in conjunction with radiotherapy and chemotherapy for cancer patients to reduce the toxic and side effects of patients during radiotherapy and chemotherapy, and promote normal function. recover. The prescription of Zhenqi preparation consists of Astragalus and Ligustrum lucidum. Invention patent 200310122216.7 discloses "Improved Preparation Method of Zhenqi Fuzheng Medicine", which can fully ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张观福
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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