Lactobacillus casei ghost as well as preparation method and application thereof

Abstract

The invention discloses a lactobacillus casei ghost, a preparation method and an application thereof, belonging to the biotechnology field. According to the lactobacillus casei ghost, lactobacillus casei is used as a research object, a recombinant vector containing cloned lytic genes is converted into the lactobacillus casei through separation of bacteriophage of the lactobacillus casei, and prediction and cloning of the lytic genes, and then the lytic genes are induced to be expressed to prepare the lactobacillus casei ghost. The lactobacillus casei ghost is used as an antigen delivery carrier safely and reliably in the true sense; besides, muramyl dipeptide in the cell wall of the lactobacillus casei has a good adjuvant effect and can remarkably enhance the immunogenicity of heterogeneous antigen; furthermore, the mass production of the lactobacillus casei ghost can be realized through fermentation, and the lactobacillus casei ghost can be preserved at room temperature for a long time after being frozen and dried, so that the cost for application of production practice is further reduced; and a new idea and a new method for further designing a novel, safe and effective drug, particularly a vaccine delivery system, which are applied to the prevention and treatment of the diseases are provided.

Description

[0001] Cross References to Related Applications [0002] This application claims the priority of the Chinese patent application filed on October 30, 2012, the application number is 201210424842.0, and the invention title is "a kind of Lactobacillus casei bacterium and its preparation method and application". methods are incorporated in this article. technical field [0003] The invention relates to a method for preparing bacterial ghosts, the bacteria ghosts prepared by the method and applications thereof. In particular, it relates to a method for preparing a Lactobacillus casei ghost and the bacterial ghost prepared by the method. Belongs to the field of biotechnology. Background technique [0004] Bacterial ghosts are empty shells of bacteria formed after the bacterial cell wall is lysed and the cell contents are removed. It has a complete bacterial surface antigen structure and can be directly used as a vaccine. At the same time, the ghost is also a good carrier, and ...

AI Technical Summary

Problems solved by technology

[0005] So far, the bacteria ghosts that have been reported are all prepared by pathogenic bacteria, and the bacteria ghost preparation technology cannot guarantee 100% complete lysis of the bacteria

Therefore, when the bacterial shadow prepared by pathogenic bacteria is used as a carrier, there is a risk of spreading pathogens and infection, so it cannot be popularized and applied in practice.

Images