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Culture medium for microcystis aeruginosa and application of culture medium

A technology of Microcystis aeruginosa and culture medium, applied in the direction of microorganism-based methods, microorganisms, bacteria, etc., can solve the problems such as the difficulty of pure cultivation of Microcystis aeruginosa in the laboratory, and achieve the effect of shortening the cultivation time

Active Publication Date: 2014-02-05
LIAONING UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] In order to solve the problem of difficult laboratory pure culture of Microcystis aeruginosa, the invention provides a culture medium and culture conditions that are beneficial to the growth of Microcystis aeruginosa

Method used

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  • Culture medium for microcystis aeruginosa and application of culture medium

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Embodiment 1

[0020] Embodiment 1 A kind of cultivation method of Microcystis aeruginosa

[0021] (1) Medium for Microcystis aeruginosa

[0022] 1.A 5 The composition of the stock solution is as follows: H 3 BO 3 2.86g / L, MnSO 4 1.59g / L, ZnSO4 7H 2 O 0.22g / L, NaMoO 4 2H 2 O 0.39g / L, CuSO 4 ·5H 2 O 0.08g / L, water balance, pH value 7.2~7.4.

[0023] Method: According to the above ratio, measure H 3 BO 3 , MnSO 4 , ZnSO4 7H 2 O, NaMoO 4 2H 2 O and CuSO 4 ·5H 2 O, dilute to 1 liter with water, pH 7.2~7.4.

[0024] 2. The composition of the culture medium is as follows:

[0025] NaCl 1.024g / L, K 2 HPO 4 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L,

[0026] Ca(NO 3 ) 2 0.077g / L, citric acid 0.006g / L, FeCl 3 0.006g / L,

[0027] Triammonium citrate 0.005g / L, EDTANa 2 0.001g / L, Na 2 CO 3 0.02g / L,

[0028] A 5 Stock solution 1mL / L, water balance.

[0029] Method: According to the above ratio, measure NaCl, K 2 HPO 4 , MgSO 4 ·7H 2 O, Ca(NO ...

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Abstract

The invention relates to a culture medium for microcystis aeruginosa and an application of the culture medium. The technical scheme is as follows: the culture medium consists of NaCl, K2HPO4, MgSO4.7H2O, Ca(NO3)2, citric acid, FeCl3, triammonium citrate, EDTANa2, Na2CO3, a A5 storage liquid and water. By adopting the culture medium and a culture method, not only can the culture time be shortened, but also the microalgae liquid is free from turning yellow in the culture process, and the frustule number of the cultured microcystis aeruginosa when reaching a growth stable stage can reach 9*10<6> / mL.

Description

technical field [0001] The present invention relates to a kind of Microcystis aeruginosa ( Microcystisaeruginosa ) culture method, including Microcystis aeruginosa culture medium improvement and culture condition optimization. Background technique [0002] With the rapid development of industry and agriculture, the discharge of nutrients into the environment is increasing day by day, and the eutrophication of water bodies is becoming more and more serious. The resulting harmful algae blooms are also becoming more frequent, which not only destroys the balance of the water ecosystem, Algae dominant in water bodies, such as Microcystis ( Microcystis ), etc. will also produce a large amount of metabolites such as algae toxins, which will have a negative impact on public health, livestock and water supply. The frequency of harmful algal blooms in my country and the world is increasing year by year. [0003] In order to explore effective ways to suppress algal blooms and bette...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 付保荣张润洁郭鑫杨玉东付豪鲁男
Owner LIAONING UNIVERSITY
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