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Method for inducing and collecting high-quality sperms of haliotis discus hannai

A method for collecting sperm and a technology for sperm, which are applied in the field of marine biology, can solve the problems of restricting the breeding and breeding of the wrinkled abalone, unable to perform artificial insemination, weak sperm motility, etc., and achieve the effects of convenient artificial insemination, high motility, and easy operation.

Active Publication Date: 2014-02-05
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the sperm viability obtained by the dissection of the wrinkled abalone is weak, artificial insemination cannot be carried out. In addition, the wrinkled abalone naturally discharges semen, and the time is not fixed and difficult to control, which seriously restricts the breeding of the wrinkled abalone.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] May is the reproductive period of wrinkled abalone, and obtained 3-4-year-old bottom-sowed wrinkled abalone from Laodong Haizhen Farm in Jiaonan, Huangdao, and selected males with a shell height of 12-15cm, full and swollen gonads, and pale yellow Parental abalones are temporarily raised; the temperature of the temporary breeding is 18-19°C, the temporary breeding density is 20 per cubic meter of water body, kelp and / or wakame are fed, the average male abalone is 10-15g / day, and the water is changed once a day. And keep it temporarily for 3-7 days, until the male abalone eats and moves normally, and the gonads are milky white, light yellow and full. Remove the male parent abalone from the holding pond, put the ventral side up in a 30cm×20cm plastic hollow basket, attach a towel soaked and wrung to half-dry on the male parent abalone, and fix it so that it cannot be shaken casually. Then place it in a quiet area away from light, and dry it in the shade for 1.5 hours.

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Embodiment 2

[0031] Male abalones with a shell height of 9-12cm, gonads plump and swollen, and light yellow in color were obtained from the Jiaonan Aquaculture Farm in June. 15 male abalones were fished out from the breeding pond and placed on a 50cm×20cm white plastic foam with the belly facing upward. In the box, a towel soaked in ultraviolet seawater and wrung to semi-dry was attached to the male abalone so that it could not be shaken casually. Place it in a quiet area away from light, dry it in the shade for 1.5 hours, take 50L of fresh seawater irradiated with a 15W UV lamp for 3 hours, place the male abalones that have been stimulated in the shade for 1.5 hours under ultraviolet light and heat up 2°C, that is, 20-21°C Put it in a quiet and dark environment to stimulate for 0.5 hours, and it was observed that the pro-abalone ejaculated successively. The collected semen was centrifuged at 3000r / min for 10 minutes to collect the turbid semen at the bottom. The collected semen has under...

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Abstract

The invention belongs to the technical field of marine organisms and particularly relates to a method for inducing and collecting high-quality sperms of haliotis discus hannai. The method comprises the steps of selection of high-quality male parent abalones, drying stimulation of the parent abalones in shade, preparation of ozone seawater, sperm collection, sperm motility detection and determination of the fertilization rate and the hatchability. According to the method, 3-4-year plump male parent abalones with gonads mature are selected, drying stimulation in shade is performed, the parent abalones are placed in seawater under the ultraviolet irradiation and arranged in the quiet and dark environment so as to release the sperms, and the high-concentration sperms are collected by centrifugation. The collected abalone sperms are subjected to artificial insemination, and the fertilization rate is over 95%. Practice proves that the abalone sperms collected in the abalone collection mode can be applied to production time and research. The method for inducing and collecting the high-quality sperms of the haliotis discus hannai is of great significance for artificial breeding, germplasm conservation and genetic diversity protection and the like of the haliotis discus hannai.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, in particular to a method for inducing and collecting high-quality spermatozoa of wrinkled abalone essence. Background technique [0002] Wrinkle plate abalone (Haliotis discushannai) belongs to Gastropoda, Primitive Gastropoda, Babaceae. It is an algae-eating marine large primitive gastropod, one of the main species of marine shellfish in northern my country, and its individual is relatively large. Abalone meat is plump and is a treasure among seafood products, and it is mostly supplied to high-end hotels and exports. In addition to eating fresh, it can be processed into canned or dried abalone, which is expensive. Abalone shells are the well-known Chinese medicine Shicai Ming, and are also important materials for making shell carving paintings. The market demand for wrinkled plate abalone is relatively large. In recent years, due to the continuous expansion of breeding scale, couple...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘清华李军韩龙江肖志忠孔宁
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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