Method for determining content of saffron in medicinal composition

A measurement method, the technology of saffron, applied in the field of medicine, can solve the problems of unstable peak time, easy tailing, and many interference components, and achieve the effects of stable measurement results, enhanced elution ability, and reduced interference

Active Publication Date: 2014-01-22
JINHE TIBETAN MEDICINE
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  • Abstract
  • Description
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Problems solved by technology

[0004] Above-mentioned HPLC measures the saffron content determination result in the saffron medicinal material and the simple composition of prescription is satisfactory, and degree of separation is better, but for the pharmaceutical composition with complex component, because there are many interfering components, it is easy to appear tailing, Baseline drift, unstable peak time, and low resolution have certain effects on the determination of saffron content

Method used

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  • Method for determining content of saffron in medicinal composition
  • Method for determining content of saffron in medicinal composition
  • Method for determining content of saffron in medicinal composition

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0031] Experimental example 1 Condition selection and methodological verification for the content determination of saffron in pharmaceutical composition by HPLC

[0032] Take Zuozhu Darcy as an example to carry out conditional selection.

[0033] 1. Instruments, reagents and test samples

[0034] Instrument: High performance liquid chromatography: Hitachi L-7110 pump, Hitachi-7420 detector; Shimadzu AUW220D electronic balance.

[0035] Reference substances: Crocin-I reference substance (batch number: 111588-200501), crocin-II reference substance (batch number: 100001-200504).

[0036] Sample: Zuozhu Daxi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) Lot number: 20090501, 20090502, 20090503.

[0037] 2. Mobile phase selection

[0038] Using the mobile phase in the prior art, i.e. methanol-water or acetonitrile-water system, to measure the content of saffron in the pharmaceutical composition with complex components, as a result, the separation R between the main p...

Embodiment 1

[0065] Example 1 A high-performance liquid chromatography method for content determination of saffron

[0066] Sample: Zuozhu Daxi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) Lot number: 20090501, 20090502, 20090503.

[0067] A high-performance liquid chromatography method for content determination of the pharmaceutical composition Zuzudaxi Chinese saffron, the steps are as follows:

[0068] Chromatographic conditions and system applicability: octadecylsilane bonded silica gel is used as filler; methanol-acetonitrile-0.05mol / L ammonium acetate is used as mobile phase, and its volume ratio is 30:5:65; detection wavelength is 440nm; The number of theoretical plates calculated based on the crocin-I peak should not be less than 4000;

[0069] Preparation of saffron reference solution: Accurately weigh crocin-I and crocin-II reference substances, add 60% methanol by volume to make each 1ml contain 30 μg crocin-I and 15 μg saffron - the mixed solution of II, to get f...

Embodiment 2

[0075] Example 2: A high-performance liquid chromatography method for content determination of Zuzudaxi Chinese saffron

[0076] Sample: Zuozhu Daxi (Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.) Lot number: 20090501, 20090502, 20090503.

[0077] A high-performance liquid chromatography method for content determination of the pharmaceutical composition Zuzudaxi Chinese saffron, the steps are as follows:

[0078] Chromatographic conditions and system applicability: octadecylsilane bonded silica gel is used as filler; methanol-acetonitrile-0.05mol / L ammonium acetate is used as mobile phase, and its volume ratio is 40:5:55; detection wavelength is 440nm; The number of theoretical plates calculated based on the crocin-I peak should not be less than 4000;

[0079] Preparation of saffron reference solution: Accurately weigh crocin-I and crocin-II reference substances, add 60% methanol by volume to make each 1ml contain 30 μg crocin-I and 15 μg saffron - the mixed soluti...

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Abstract

The invention discloses a method for determining the content of saffron in a medicinal composition, and aims to solve the problem that the content of saffron in a complex medicinal composition is difficult to determine. According to the method, methanol-acetonitrile-0.05mol / L ammonium acetate in a volume ratio of (30-45):5:(50-65) is adopted as a mobile phase, and a high performance liquid chromatography is adopted for determination; the mobile phase is a multi-solvent system, the mobile phase system is kept to be stable by adopting buffer salt, and the elution power is reinforced. The method provided by the invention is stable in determination result and good in linearity, the correlation coefficient, the resolution and the number of theoretical plates can meet the requirement of measurement standard, and a good detection result can be obtained on the saffron in a medicinal composition with a complex prescription.

Description

technical field [0001] The invention relates to a method for determining the content of a traditional Chinese medicine compound preparation, in particular to a method for determining the content of saffron in a pharmaceutical composition, and belongs to the technical field of medicine. Background technique [0002] Saffron, also known as saffron, saffron, and the stigma of Crocus sativus L., a plant of the family Iridaceae, is a famous and precious Chinese medicinal material with strong physiological activities, sedative, expectorant, and antispasmodic effects. As a precious medicinal material, saffron is used in many prescriptions, especially in ethnic medicines such as Tibetan medicine, such as Zhuzhu Daxi, Renqing Changjue, Ganchang Capsule, Zhenlong Xinnao Capsule, Twenty-five flavors of coral balls, etc. [0003] At present, there are many methods for determining the content of saffron, such as ultraviolet spectrophotometry, high-performance liquid chromatography, etc....

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 马宏伟赵延霞孙绪丁任松鹏魏永义
Owner JINHE TIBETAN MEDICINE
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