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Separation method of sexed semen

A separation method and technology for semen, applied in the field of animal reproduction, can solve problems such as unsatisfactory semen separation effect, uneven dyeing of original semen, etc., and achieve the effect of ensuring uniformity and stability and ensuring smooth progress.

Active Publication Date: 2014-01-22
大连金弘基种畜有限公司
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Problems solved by technology

[0003] In related technologies, in the method of separating sexually controlled semen, the common operation is to directly dye the original sperm after detecting their vitality, and then separate them after dyeing; however, because the original sperm generally contains large sperm masses and some sundries , these sperm clumps and debris often lead to uneven staining of the original sperm, resulting in unsatisfactory separation of semen containing X chromosomes and semen containing Y chromosomes

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  • Separation method of sexed semen
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Embodiment 2

[0023] Step 201: Filter the original semen through a 40-60 micron filter for the first time to obtain the filtered semen from which the sperm mass and sundries are removed;

[0024] Step 202: Take 15-25 microliters of filtered semen and drop them on a glass slide preheated at 32°C-36°C for 0.5-1.5 minutes, then observe the viability of the filtered semen under a microscope at 200-400 times;

[0025] Specifically, in the process of detecting vitality, it can be carried out through the operation of step 202; in addition, in the specific process of measuring vitality, the semen that meets the separation standard can have a vitality of 0.6-0.85 and a density ≥ 1 billion / ml, which is the standard , for the filtered semen with a density between 600 and 800 million / ml, it can be centrifuged to make the density reach the separation standard before staining.

[0026] Step 203: Preheat the Staining Talp solution and the 4% Egg yolk Talp solution at a temperature of 32°C-36°C for 10-20 m...

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Abstract

The invention relates to the field of animal breeding, in particular to a separation method of sexed semen. The separation method comprises the steps as follow: an original semen is primarily filtered by a 40-60 mu m filter, and then a filtered semen free of spermatium and impurities is obtained; the vitality of the filtered semen is detected to obtain a to-be-stained filtered semen with the vitality ranging from 0.6-0.85; the to-be-stained filtered semen with the vitality ranging from 0.6-0.85 is stained to obtain a stained semen; and the stained semen is separated by a flow cytometry to obtain a semen containing X-chromosomes and a semen containing Y-chromosomes. With the adoption of the separation method of the sexed semen, uniformity and stability of staining of the to-be-stained filtered semen can be guaranteed, and then the efficient separation of the semen containing the X-chromosomes and the semen containing the Y-chromosomes is facilitated; and besides, the filtered semen has low possibility of blocking a nozzle of the flow cytometry, so that the whole separation process can be performed smoothly.

Description

technical field [0001] The invention relates to the field of animal reproduction, in particular to a method for separating sexually controlled semen. Background technique [0002] Animal sex control (sex control) technology is a biological technology that makes adult female animals produce offspring of the desired sex by artificially intervening in the normal reproductive process of animals; specifically, this technology uses animals containing X chromosomes and The difference in DNA content in the semen containing the Y chromosome is stained with a specific dye, and then according to the intensity of the emitted fluorescence, the type of sperm is judged by a computer, and then by adding charges to the semen droplets, according to the electrostatic principle, the semen containing the X chromosome It is separated from the semen of the Y chromosome and selectively used in animal reproduction. [0003] In related technologies, in the method of separating sexually controlled se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
Inventor 帅志强苏冠宇
Owner 大连金弘基种畜有限公司
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