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Coccidiosis trivalent live vaccine and preparation method and application thereof

A chicken coccidiosis and live vaccine technology, applied in the field of immunobiology and biopathology, can solve the problems of shortened endogenous development cycle, inability to produce immunity, and weak virulence, so as to achieve good immune effect and promote improvement and promotion, the effect of high protection rate

Inactive Publication Date: 2014-01-22
西安天星生物药业股份有限公司
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AI Technical Summary

Problems solved by technology

Usually, the parental strains are repeatedly passaged and screened to shorten the endogenous development cycle of the strains, forming precocious strains with biological characteristics different from the parental strains, which are used to produce attenuated vaccines, but precocious strains also have disadvantages, such as immunogen High sex, correspondingly weak virulence, unable to produce better immunity, etc.

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  • Coccidiosis trivalent live vaccine and preparation method and application thereof

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Embodiment Construction

[0034] In the following examples, the applicant provides a specific preparation process of the vaccine of the present invention, which is only used as a preferred example, without using the specific dosage, time, intensity, temperature and other parameters used in the following methods, and other methods can also be used .

[0035] Adopt following process to prepare live vaccine of the present invention:

[0036] 1. Separate, purify and cultivate three types of Eimeria soft, poisonous and giant respectively.

[0037] (1) Separation of coccidia oocysts: add 380 g of salt to 1000 ml of boiled water, and stir fully to obtain a saturated saline solution with a specific gravity of about 1.18. Stir the feces and physiological saline 5 times the volume of the feces into a suspension, filter the feces suspension through a 50-mesh sieve first, and then filter it through a 100-200-mesh sieve into a second container, and place Pour the filtrate in the second container into a centrifuge...

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Abstract

The invention discloses a coccidiosis trivalent live vaccine which is prepared by performing first-stage attenuation on spore ball egg sacs which are obtained by performing separation, purification and cultivation on three types of eimeriida, namely eimeria tenella, eimeria necatrix and eimeria maxima, through alkylating agent chemical mutagen and mixing three second-stage attenuation plants obtained by performing second-stage attenuation by irradiation of physical mutagen ultraviolet on oocysts of chicks cultivated by first-stage attenuation plants. Furthermore, the invention also discloses a preparation method and application of the coccidiosis trivalent live vaccine. By adopting dual attenuation, the coccidiosis trivalent live vaccine disclosed by the invention is high in induction rate, low in toxicity and stable in immunity.

Description

technical field [0001] The invention relates to a vaccine for immunizing diseases caused by coccidia, belonging to the fields of immunobiology and biopathology. Background technique [0002] Chicken coccidiosis is a kind of protozoan disease that seriously harms the intensive chicken industry. The disease is caused by 9 species of Eimeria coccidia of Sporozoa, Eimeriaceae, and Eimeria. It is reported that chicken coccidiosis causes up to 3 billion U.S. dollars in economic losses every year in the world, and the annual direct and indirect economic losses caused by coccidiosis in the United States are about 640 million U.S. dollars, while the annual cost of anticoccidiosis drugs in my country is about It is 600-800 million yuan, accounting for nearly one-third of the total cost of chicken disease prevention and control. With the rapid development of my country's intensive chicken industry, this figure will rise sharply. [0003] Because coccidia are transmitted through the sim...

Claims

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Application Information

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IPC IPC(8): A61K39/012A61K39/39A61P33/02
Inventor 李丹青贾妮
Owner 西安天星生物药业股份有限公司
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