Preparation method of high-activity buckwheat leaf flavonoids

A technology of buckwheat leaf flavonoids and high activity, which is applied in the field of preparation of high-activity buckwheat leaf flavonoids, can solve the problems of low development and utilization of buckwheat leaves, reduced safety, expensive enzyme preparations, etc., and achieves improved absorption and bioavailability and low cost , good fat-soluble effect

Inactive Publication Date: 2014-01-22
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical hydrolysis introduces chemical reagents into the reaction system, which increases toxicity and reduces safety; although enzymatic hydrolysis is green and efficient, there is still no suitable enzyme source at present, and enzyme preparations are expensive. In contrast, high-pressure hydrolysis is a preferred method. Program
Buckwheat is widely planted in my country, but the development and utilization of buckwheat leaves is very low, and they are basically burned as firewood

Method used

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  • Preparation method of high-activity buckwheat leaf flavonoids
  • Preparation method of high-activity buckwheat leaf flavonoids
  • Preparation method of high-activity buckwheat leaf flavonoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of buckwheat leaf flavonoids crude extract: wash and dry the buckwheat leaves harvested in the field, grind them, and pass through a 60-mesh sieve to obtain buckwheat leaf powder; weigh 40 g of buckwheat leaf powder, add 1600 mL of 85% edible ethanol (solid-liquid ratio 1:40), mix well, put it in a 70°C water bath, stir and extract for 4 hours, take it out, filter while hot to obtain the filtrate, wash the precipitate once with 2 times of 85% edible ethanol, combine the filtrate; concentrate with a rotary evaporator When the filtrate reached 300mL, a large amount of precipitation was precipitated; the concentrated solution was centrifuged, and the precipitate was dried and crushed at 60°C to obtain 5.33g of buckwheat leaf flavonoid crude extract, with a total flavonoid content of ≥73.5%.

[0023] Determination of flavonoid components and content by high performance liquid chromatography: Instrument: 1100 series high performance liquid chromatography from ...

Embodiment 2

[0032]Preparation of highly active buckwheat leaf flavonoids: Wash and dry the buckwheat leaves harvested in the field, grind them, and pass through a 60-mesh sieve to obtain buckwheat leaf powder; weigh 40g of buckwheat leaf powder, add 85% edible ethanol 2000mL (solid-liquid ratio 1 :50), mix well, put into 70 DEG C of water baths and stir and extract for 4h, after taking it out, suction and filter while hot to obtain the filtrate, wash the precipitate once with 2 times of 85% ethanol, combine the filtrate; concentrate the filtrate to 200mL, put it into a high-pressure reactor, and hydrolyze it at 0.7MPa for 20min. After the reactor cools down, pour the hydrolyzate into a centrifuge cup, collect the precipitate by centrifugation at 6000g, wash the precipitate with distilled water 4 times, and dry the obtained precipitate at 60°C to obtain Buckwheat leaf flavonoid products with higher physiological activity, the total flavonoid content of the product is ≥88.8%.

[0033] The a...

Embodiment 3

[0036] Preparation of highly active buckwheat leaf flavonoids: Wash and dry the buckwheat leaves harvested in the field, grind them, and pass through a 60-mesh sieve to obtain buckwheat leaf powder; weigh 40g of buckwheat leaf powder, add 1600mL of 85% edible ethanol (solid-liquid ratio 1 :40), mix well, put it in a 70°C water bath, stir and extract for 4 hours, take it out, and filter while hot to obtain the filtrate, wash the precipitate once with 2 times of 85% edible ethanol, and combine the filtrate; use a rotary evaporator to concentrate the filtrate to 160mL, put it into a high-pressure reactor, and hydrolyze it at 0.7MPa for 60 minutes. After the reactor cools down, pour the hydrolyzate into a centrifuge cup, collect the precipitate by centrifugation at 6000g, wash the precipitate with distilled water 4 times, and dry the precipitate at 60°C. Obtain a buckwheat leaf flavonoid product with higher physiological activity, and the total flavonoid content of the product is ≥...

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Abstract

The invention discloses a preparation method of high-activity buckwheat leaf flavonoids. The preparation method comprises the following steps: extracting in 65-75 DEG C water bath by using 75-85% edible ethyl alcohol as a solvent according to a ratio of material to liquid being 1: (30-50), concentrating suction filtration liquid to one eighth to one tenth of the original volume, putting in a high-pressure reaction kettle, hydrolyzing for 20-60 minutes under the vapor pressure of 0.6-0.8Mpa, centrifuging, collecting precipitates, and drying at 60 DEG C, thus obtaining the high-activity buckwheat leaf flavonoid product. The purity of the buckwheat leaf flavonoid product is increased to 90.6% from 73.5% before high-pressure hydrolysis, and the removal ratio of DPPH free radicals is increased by 9.7% compared with the removal ratio before hydrolysis. Due to the adoption of the preparation method, the content of isoquercetin and quercetin which have relatively high biological activity is increased, the content of the isoquercetin is increased to 3.27-20.6% from zero before hydrolysis, and the content of the quercetin is increased to 8.2-22.78% from 2.2% before hydrolysis. The preparation method has the advantages of waste utilization, low cost, no pollution, simple operation and the like.

Description

technical field [0001] The invention relates to buckwheat leaf flavonoids, in particular to a preparation method of highly active buckwheat leaf flavonoids and a product thereof. Background technique [0002] Buckwheat belongs to the genus Buckwheat of Polygonaceae, a dicotyledonous plant, and buckwheat grains, roots, stems, leaves and flowers are rich in flavonoids. The main component of flavonoids in buckwheat leaves is rutin, and a small amount of quercetin. Chemical structure analysis reveals that rutin is the rutinoside of quercetin, isoquercetin is the glucoside of quercetin, rutin can be hydrolyzed to isoquercetin or quercetin, and isoquercetin can be hydrolyzed to quercetin corticosteroids. The structural formulas of quercetin, isoquercetin and rutin are as follows: [0003] [0004] The three of them have different bioavailability and physiological activities. Cell biology studies have shown that the absorption and utilization of flavonoids mainly enter the bl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L33/00
CPCA61K36/70A61K2236/15A61K2236/30A61K2236/333A61K2236/53
Inventor 李艳琴史建强李彬春
Owner SHANXI UNIV
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