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LLarge scale human urine protein collection method

A urine protein and human collection technology, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., to prevent denaturation and inactivation, expand the collection range, and have strong controllable quality effects.

Inactive Publication Date: 2014-01-15
YANGZHOU AIDEA BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above-mentioned method of collecting urine protein from urine is used in the production of urine protein raw materials. In order to realize large-scale and industrial production, it is necessary to accumulate enough batches of adsorbents to be transported to processing plants for processing. After accumulating a sufficient amount of adsorbents, When storing the adsorbent, it is necessary to solve the problem of reducing the activity of urinary protein, protein degradation, and controlling microbial growth.

Method used

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  • LLarge scale human urine protein collection method
  • LLarge scale human urine protein collection method
  • LLarge scale human urine protein collection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 24 hours experimental temperature 16 ～ 22°C, 150Kg macroporous anion resin (ligand is quaternary ammonium group (-N ＋ R 3 ) polystyrene resin) regenerated by acid and alkali, and then packed in 50-mesh filter bags according to the amount of 0.5-5Kg / bag, and then placed in a urinal or urinal for online adsorption of urine protein, collected after 24 hours , transported back to the processing point and mixed evenly, and the evenly mixed resin was divided into A, B, and C three parts, each 50Kg. Among them, A, after rinsing with clear water and centrifugal dehydration, put it in a cold storage for 10 days; B, after rinsing with clear water, put it in a cold storage for 10 days; Precipitate, filter with diatomaceous earth, and blow dry to obtain 215 g of crude urine protein product, containing 41,500 U / g of urinary trypsin inhibitor and 1.20 PNAU / g of human urinary kininogenase.

[0061] After 10 days, resins A and B were eluted with 1mol / L sodium chloride solution respe...

Embodiment 2

[0066] 24 hours experimental temperature 22 ～ 31°C, put 100Kg macroporous anion resin (ligand is quaternary ammonium group (-N ＋ R 3 ) polystyrene resin) regenerated by acid and alkali, and then packed in 50-mesh filter bags according to the amount of 0.5-5Kg / bag, and then placed in a urinal or urinal for online adsorption of urine protein, collected after 24 hours Transport to the processing point, use a high-pressure cleaner to generate high-pressure water for cleaning, store in a cold storage for 10 days, elute with 1mol / L sodium chloride solution, then concentrate by ultrafiltration, precipitate ammonium sulfate, filter with diatomaceous earth, and dry to make The urine protein crude product is 390g, containing 35,000 U / g of human urinary trypsin inhibitor. Through the component analysis of this crude product, although there are urinary trypsin inhibitor main component peaks (see Figure 4 , retention time 11.540min), but the degradation is still very serious (see Fig...

Embodiment 3

[0069] 24 hours experimental temperature 22 ～ 32°C, 100Kg macroporous anion resin (ligand is quaternary ammonium group (-N ＋ R 3 ) polystyrene resin) regenerated by acid and alkali, and then packed in 50-mesh filter bags according to the amount of 0.5-5Kg / bag, and then placed in a urinal or urinal for online adsorption of urine protein, collected after 24 hours Transport to the processing point, use a high-pressure cleaner to generate high-pressure water to clean, centrifuge and dehydrate, and store in a cold storage for 10 days. According to the method of Example 2, 410 g of crude urine protein was obtained, containing 38,000 U / g of human urinary trypsin inhibitor. Through the component analysis of this crude product, although there is an obvious main component peak of urinary trypsin inhibitor (see Figure 5 , retention time 11.648min), but the degradation is still serious.

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Abstract

The invention discloses a large scale human urine protein collection method. The method comprises the following steps: placing absorption agents, which are used for absorbing urine proteins and have been packaged, in the places where urine will pass through to make the absorption agent be able to absorb the urine proteins in the urine; collecting the absorption agents which have absorbed the urine proteins; subjecting the collected absorption agents to a pretreatment; refrigerating and storing the pre-treated absorption agents; and transporting the absorption agents to a processing factory in batches to carry out urine protein desorption process to produce corresponding urine protein raw materials or urine protein products. The large scale human urine protein collection method is suitable for the mass industrial urine protein production, improves the processing amount of urine protein, reduces the transportation cost of absorption agents and the production cost of urine proteins, furthermore, the absorption agents containing urine proteins are subjected to a pre-treatment before storage, thus the urine protein activity is preserved, the urine protein degradation is reduced, the microorganism generation is reduced, so that the urine protein product quality is improved.

Description

technical field [0001] The invention relates to a method for large-scale collection of human urinary protein, which is mainly used in the production of crude urine protein raw materials such as human urinary trypsin inhibitor, human urinary kininogenase, human urinary albumin, and human urokinase. Background technique [0002] Human urine is an important source of human protein. Human urine contains hundreds of proteins, and dozens of proteins have been proven to have important medicinal value. Currently only Human Urinary Trypsin Inhibitor (Human Urinary Trypsin Inhibitor), Urinary Kallidinogenase (Urinary Kallidinogenase), Urokinase (Urokinase), HMG (Urinary Gonadotropin), FSH (Follitropin), HCG (Chorionic gonadotropin) and a few functional urine protein products have been successfully developed, and have played a very important role in the treatment of acute pancreatitis, shock and acute cerebral infarction, myocardial infarction and other clinical critical diseases, art...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/81C07K14/76C07K1/14C12N9/64C12N9/72
CPCC07K14/81C07K14/76C12N9/6445C12N9/6462C12Y304/21008C12Y304/21073
Inventor 苗丕渠张纪兵苏古方俞恒许冬至
Owner YANGZHOU AIDEA BIOTECH
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