Method for collecting urine protein in large scale from human urine
A urine protein and large-scale technology, which is applied in the field of large-scale collection of urine protein, can solve the problems affecting the market development of urine protein products, the difficulty of urine collection, and the high cost of collection and transportation, so as to increase the single production batch and reduce the Effects of transportation cost and expansion of collection range
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Embodiment 1
[0044] Method for large-scale collection of human urine protein:
[0045] a) Place the pre-packed adsorbent for absorbing urine protein in the place where the urine flows through to absorb the urine protein in the urine;
[0046] b) Collect the adsorbent for adsorbing urinary protein, and add ice to cool when the ambient temperature is higher than 20°C during the process of collecting the adsorbent;
[0047] c) Before storing the adsorbent that absorbs urinary protein, perform pretreatment. The pretreatment method is to wash the adsorbent with high-pressure water, soak it in clean water, and dehydrate it, and dehydrate it with a centrifuge or a dehydrator; store the adsorbent in cold storage after dehydration ; Used to remove residual urine, microorganisms and urine scale from the adsorbent. It is used to prevent the denaturation and inactivation of urinary protein in the adsorbent, or even degrade it, and reduce the growth of microorganisms. According to the situation, the a...
Embodiment 2
[0051] On the basis of Example 1, when the preferred adsorbent device is an anion exchange resin, a urine filter pad is made. The urine filter pad is made of a nylon filter cloth with good permeability and waterproof, which is sewn into a bag and anion exchange resin in the bag. The preparation method is as follows: the suitable sieve size of the filter cloth is 300 mesh or less, and the pore size is more than 50 mesh, preferably 60 mesh pore size. The shape and size of the filter pad are compatible with the bottom of the urinal or trough, and can be square, round, polygonal, etc.; the side length or diameter of the filter pad used for the urinal is preferably 20-30cm; The filter pad used in the urinal is laid flat on the bottom of the small urinal, and its size should be able to fully cover the bottom of the urinal; the urine flows through the filter pad before flowing out of the urinal.
[0052] Take a macroporous anion exchange resin, for example, the ligand is {-N+(CH 3 ...
Embodiment 3
[0057] On the basis of Example 1, a 30cm×30cm size filter bag is sewn with a pore size of 80 mesh filter cloth, and the ligand used is -N(CH 3 ) 3 2 kilograms of the macroporous anion exchange resin styrene-diethylene copolymer of OH, through 2% NaOH treatment, water flushes to neutrality, packs in the filter cloth bag, makes urine filter pad.
[0058] Place the urine filter pad in the urine funnel, replace the filter pad for 24 hours, collect the resin, and desorb according to the method described in Example 1. The elution solution contained 1.28 grams of urinary protein.
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