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Epulorhiza sp. strain, application thereof and mycorrhiza fungi inoculation method

A technology for mycorrhizal fungi and strains is applied in the field of mycorrhizal fungi inoculation method, which can solve the problems that have not been seen before, and achieve the effects of increasing biomass and polysaccharide content, promoting growth and high economic benefits.

Inactive Publication Date: 2014-01-08
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

[0005] At present, there are no reports of this Epulorhiza sp. strain S1 (CGMCC No.8145) and its application in promoting the growth of Dendrobium candidum and its application in promoting the accumulation of polysaccharides in Dendrobium candidum in the prior art

Method used

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  • Epulorhiza sp. strain, application thereof and mycorrhiza fungi inoculation method
  • Epulorhiza sp. strain, application thereof and mycorrhiza fungi inoculation method
  • Epulorhiza sp. strain, application thereof and mycorrhiza fungi inoculation method

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Embodiment 1

[0024] 1Materials and methods

[0025] 1.1 Separation of endophytic fungi

[0026] Take the healthy roots of wild Dendrobium officinale (collected from the subtropical forest of Xishuangbanna, Yunnan in April 2009), wash them with water, put them in a 0.1% mercury liter solution on an ultra-clean workbench, soak for 8-10 minutes, and rinse with sterile water for 5 Next, blot dry with sterile filter paper, cut into 0.5-1cm sections in a sterile petri dish, place them on PDA plate culture medium, put 8 sections in each dish, cultivate in the dark at 25℃. When the hyphae grow from the root tissue and form a colony of a certain size, pick the hyphae from the edge of the colony and transfer to another PDA plate for pure culture. Then it was transferred to PDA slant medium, cultivated for 10-15 days, and stored in a refrigerator at 4°C. Through in vitro inoculation experiments, it was detected that S1 strain had a good promoting effect on Dendrobium candidum.

[0027] 1.2 Molecular biol...

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Abstract

The invention relates to an epulorhiza sp. strain S1 (CGMCC No.8145) and application of the epulorhiza sp. strain S1 in promoting of the growth of dendrobium officinale and the accumulation of dendrobium officinale polysaccharides. According to the application, a dendrobium officinale tissue culture seedling is taken as a material and is manually inoculated with the S1 (epulorhiza sp.) strain, after the tissue culture seedling and the S1 strain are co-cultured for 60 days, S1 fungus and the root of a host form a symbiotic relationship, the fresh weight increment, dry weight, plant height, stem height, stem diameter, number of nodes, internode length, number of tillers, number of fresh roots and polysaccharide content of the tissue culture seedling are remarkably higher than a contrast, and the polysaccharide content is 141.63% higher than the content of an uncultured seedling and is about 2.4 times of the polysaccharide content of the uncultured seedling. By utilizing a novel technique, the growth of the dendrobium officinale tissue culture seedling is greatly promoted, and the biomass and the polysaccharide content of the dendrobium officinale tissue culture seedling are increased; according to a technical method, the operability is strong, the high-quality dendrobium officinale tissue culture seedling can be produced, and high economic benefits are brought to an efficient cultivation technique and industrialization production of the dendrobium officinale.

Description

Technical field: [0001] The present invention belongs to the field of biotechnology, and in particular, relates to a strain S1 (CGMCC No. 8145) of Epulorhiza sp. and its application in promoting the growth and polysaccharide accumulation of Dendrobium officinale and the mycorrhiza using it Fungus inoculation method. Background technique: [0002] Dendrobium is the second largest genus in the Orchidaceae family. There are about 1,100 native species of Dendrobium in the world, mainly distributed in South Asia, East Asia and Southeast Asia (Wang et al., 2011; Chen et al., 2010). There are 81 species and 2 varieties in China (Wu Chengyong and Liu Yan, 2010). Many plants of the genus Dendrobium have high medicinal value, such as Dendrobium officinale, Dendrobium nobile and Dendrobium Dendrobium, etc. (People's Republic of China Pharmacopoeia Committee, 2010). [0003] Dendrobium officinale is a valuable medicinal plant in the Dendrobium genus Orchidaceae, which is mainly distributed ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H4/00
Inventor 严宁王秋霞纪大干李树云胡虹
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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