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Mycovirus, phytopathogenic fungus, plant disease controlling agent, method for controlling plant disease, and method for attenuating phytopathogenic fungus

A plant disease control and plant disease technology, applied in the field of novel fungi and viruses, can solve the problems of economic blow, quality decline, rice harvest failure, etc., and achieve the effects of excellent efficiency, excellent control, and excellent control effect.

Inactive Publication Date: 2014-01-01
NAT UNIV CORP TOKYO UNIV OF AGRI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, due to meteorological factors such as low temperature, rainy weather, and insufficient sunshine in summer, a large number of meteorological factors occur, resulting in poor harvest and quality decline of rice, which brings a huge blow to the economy.

Method used

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  • Mycovirus, phytopathogenic fungus, plant disease controlling agent, method for controlling plant disease, and method for attenuating phytopathogenic fungus
  • Mycovirus, phytopathogenic fungus, plant disease controlling agent, method for controlling plant disease, and method for attenuating phytopathogenic fungus
  • Mycovirus, phytopathogenic fungus, plant disease controlling agent, method for controlling plant disease, and method for attenuating phytopathogenic fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] In this example, according to the method disclosed in International Publication No. WO / 2009 / 093409, the rice blast strain S-0412-II 2a strain containing a novel fungal virus was isolated and identified. Specifically, the rice blast fungus S-0412-II 2a strain isolated and identified in this example and the rice blast fungus S-0412- II 1a strain, colony observation. A PDA medium was used for culturing these rice blast fungus. see results figure 1 .

[0101] Such as figure 1 As shown, the rice blast strain S-0412-II 2a strain identified in this example showed albino flora, and growth inhibition was observed. In addition, conidia formation was not observed at all. In addition, rice blast bacterial strain S-0412-II 2a strain is different from rice blast bacterial strain S-0412-II 1a strain, and it has formed colony on PDA medium, and the characteristic of this colony is: pigmentation (melanin) will not occur. (Chem), showing abnormal growth failures such as growth in...

Embodiment 2

[0107] In this example, it was studied whether the MoCV3 contained in the rice blast strain S-0412-II 2a isolated and identified in Example 1 still exists outside the bacterium.

[0108] In Example 1, among the strains in which the bands of endogenous double-stranded RNA of five components were detected at 2.8 to 3.6 kb, three strains were transplanted into a liquid medium for culture, and then the culture medium was centrifuged to recover the culture medium. supernatant. Next, the obtained culture supernatant was electrophoresed on 1% agarose gel at 20 V for 18 hours, and stained with ethidium bromide.

[0109] As a result, a double-stranded RNA band was detected at the same position as the endogenous double-stranded RNA of the bacteria in the culture supernatant of the above strain. This result shows that: the fungal virus of the present invention exists not only in the fungus of rice blast fungus but also in the outside of the fungus.

Embodiment 3

[0111] In this example, it was investigated whether the mycovirus MoCV3 existing outside the bacteria has the ability to infect normal strains (strains that do not retain mycoviruses).

[0112] First, rice blast fungus strain S-0412-II 2a was transplanted into a liquid culture medium, and after 4 weeks of culture, the culture liquid was centrifuged to recover the culture supernatant. The culture supernatant was subjected to electrophoresis on 1% agarose gel according to the same procedure as in Example 2, and as a result, a double-stranded RNA band was confirmed. The obtained culture supernatant was filter-sterilized using a 0.22 μL filter.

[0113] Next, put 50ml of YG medium into a 100ml flask (Kolben), inoculate a normal strain of rice blast fungus (a bacterial strain that does not retain the mycovirus) therein, and after cultivating for 3 days, add 500 μL of the resulting culture supernatant, and observe Bacterial growth.

[0114] As a result, on the third day of observa...

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Abstract

A mycovirus, which exerts an improved controlling effect on a phytopathogenic fungus, characterized by having 5 kinds of double-stranded RNAs and 4 kinds of double stranded RNAs, among the aforesaid 5 kinds of double-stranded RNAs, having homology of at least 81%, 75%, 72% and 73% respectively to base sequences represented by SEQ ID NOS:1-4. As an example thereof, a novel mycovirus MoCV3 is utilized.

Description

technical field [0001] The present invention relates to a novel mycovirus that suppresses the infectivity of plant disease fungi to plants, and to a plant disease fungus infected with the mycovirus, a plant disease control agent and a plant disease control method using the mycovirus, and a plant disease control method using the fungus Viral plant disease fungus attenuation method. Background technique [0002] Among plant diseases, there are diseases caused by environmental factors such as weather and soil, diseases caused by infectious factors such as viruses, bacteria and fungi (filamentous fungi), diseases caused by physiological disorders, and diseases caused by a combination of these factors disease etc. Currently, plant diseases include many diseases that hinder the production of food, flowers, trees, trees, etc., and there are also many diseases that have a large economic impact. [0003] In plant diseases, fungi are one of the most important disease factors. It is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A01N63/00A01P3/00C12N1/14A01N63/40
CPCC12N7/00C07K14/08C12R1/645A01N63/00C12N2720/00021C12N1/14C12N2720/00031A01N63/04A01N63/40C12R2001/645C12N1/145
Inventor 森山裕充福原敏行有江力寺冈彻
Owner NAT UNIV CORP TOKYO UNIV OF AGRI & TECH
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